Phox2b^em3(cre)Heno
Endonuclease-mediated Allele Detail

Summary

• Symbol: Phox2b^em3(cre)Heno
• Name: paired-like homeobox 2b; endonuclease-mediated mutation 3, Hideki Enomoto
• MGI ID: MGI:7657398
• Synonyms: Phox2b^Cre
• Gene: Phox2b Location: Chr5:67251751-67256399 bp, - strand Genetic Position: Chr5, 35.95 cM
• Alliance: Phox2b^em3(cre)Heno page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Phox2b^em3(cre)Heno expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Phox2b (MGI:1100882)

• Mutation details: CRISPR/cas9 mediated recombination using guide RNAs and a donor vector inserted a cre/ERT2-ipA cassette into exon 1 around the first ATG of the gene. This was further modified by CRISPR/cas9 mediated recombination to insert a stop codon in the junction region between the cre and ERT2 sequences. (J:344865)
• Inheritance: Not Specified

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Phox2b (mouse) Summary of all recombinase alleles driven by Phox2b. Alliance mouse Phox2b gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Phox2b Mutation: 25 strains or lines available

References

• Original: J:344865 Putra BP, et al., Pcgf1 gene disruption reveals primary involvement of epigenetic mechanism in neuronal subtype specification in the enteric nervous system. Dev Growth Differ. 2023 Oct;65(8):461-469
• All: 1 reference(s)