Trim66^em1Emr
Endonuclease-mediated Allele Detail

Summary

• Symbol: Trim66^em1Emr
• Name: tripartite motif-containing 66; endonuclease-mediated mutation 1, EMBL Rome
• MGI ID: MGI:7657542
• Synonyms: Trim66^em2(gfp)Emr, Trim66^gfp
• Gene: Trim66 Location: Chr7:109048213-109107341 bp, - strand Genetic Position: Chr7, 57.21 cM
• Alliance: Trim66^em1Emr page

Mutation origin

• Strain of Origin: FVB/NCrl

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 technology inserted an in-frame EGFP, a stop codon, and SV40 poly-A-signal in exon 3 at genomic position 109,083,675 (GRCm39). The insertion of a premature stop codon in frame of the exon encoding Bbox generates a loss-of-function allele. Western blot analysis confirmed absence of protein in testis of homozygotes. No EGFP expression is detected in adult testicular cells, indicating that the EGFP mRNA is not translated. (J:349240)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trim66 Mutation: 58 strains or lines available

References

• Original: J:349240 Mielnicka M, et al., Trim66's paternal deficiency causes intrauterine overgrowth. Life Sci Alliance. 2024 Jul;7(7)
• All: 1 reference(s)