Ctps1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ctps1^{tm1c(KOMP)Wtsi}
• Name: cytidine 5'-triphosphate synthase 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7657751
• Synonyms: Ctps1^flox
• Gene: Ctps1 Location: Chr4:120397065-120427473 bp, - strand Genetic Position: Chr4, 56.52 cM
• Alliance: Ctps1^{tm1c(KOMP)Wtsi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:348981
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 120419414 of Chromosome 4 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 120420614. The critical exon 4 is thus flanked by loxP sites. The lacZ and neomycin resistance gene were removed via flp-mediated recombination generating a conditional ready exon 4 floxed allele. (J:348981)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ctps1 Mutation: 46 strains or lines available

References

• Original: J:348981 Soudais C, et al., Inactivation of cytidine triphosphate synthase 1 prevents fatal auto-immunity in mice. Nat Commun. 2024 Mar 4;15(1):1982
• All: 1 reference(s)