Ctps2^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ctps2^{tm1d(EUCOMM)Hmgu}
• Name: cytidine 5'-triphosphate synthase 2; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7657754
• Synonyms: Ctps2^ko
• Gene: Ctps2 Location: ChrX:161684234-161815504 bp, + strand Genetic Position: ChrX, 75.09 cM, cytoband F5
• Alliance: Ctps2^{tm1d(EUCOMM)Hmgu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:348981
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_GT0_LF2A_LacZ_BetactP_neo cassette was inserted at position 161708956 of Chromosome X upstream of the critical exon 8 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 8 at position 161710712. The critical exon 8 is thus flanked by loxP sites. The lacZ and neomycin resistance gene were removed via flp-mediated recombination. Exon 8 was deleted via cre-mediated recombination, generating a knock-out allele. (J:348981)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ctps2 Mutation: 17 strains or lines available

References

• Original: J:348981 Soudais C, et al., Inactivation of cytidine triphosphate synthase 1 prevents fatal auto-immunity in mice. Nat Commun. 2024 Mar 4;15(1):1982
• All: 1 reference(s)