Slc13a3^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc13a3^{tm1c(KOMP)Wtsi}
• Name: solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:7660528
• Synonyms: Slc13a3^flox
• Gene: Slc13a3 Location: Chr2:165247215-165315117 bp, - strand Genetic Position: Chr2, 85.54 cM, cytoband H3
• Alliance: Slc13a3^{tm1c(KOMP)Wtsi} page

Mutation origin

• Mutant Cell Line: EPD0110_2_E02
• Germline Transmission: Earliest citation of germline transmission: J:349783
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 165287182 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 165288050. The critical exon(s) is/are thus flanked by loxP sites. The lacZ and neomycin resistance gene were deleted via flp-mediated recombination creating a conditional ready floxed allele. (J:349783)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc13a3 Mutation: 34 strains or lines available

References

• Original: J:349783 Hull VL, et al., Astroglial conditional Slc13a3 knockout is therapeutic in murine Canavan leukodystrophy. Ann Clin Transl Neurol. 2024 Apr;11(4):1059-1062
• All: 1 reference(s)