Leprot^tm1.2Damj
Targeted Allele Detail

Summary

• Symbol: Leprot^tm1.2Damj
• Name: leptin receptor overlapping transcript; targeted mutation 1.2, Julie Dam
• MGI ID: MGI:7662966
• Gene: Leprot Location: Chr4:101504922-101516561 bp, + strand Genetic Position: Chr4, 46.94 cM
• Alliance: Leprot^tm1.2Damj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:350377
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 101509123 of Chromosome 4 upstream of the exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 2 at position 101509956. The exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml Absence of expression of RNA and protein was confirmed in multiple tissues from homozygous mice. (J:349250, J:350377)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Leprot Mutation: 16 strains or lines available

References

• Original: J:350377 Vauthier V, et al., Endospanin1 affects oppositely body weight regulation and glucose homeostasis by differentially regulating central leptin signaling. Mol Metab. 2017 Jan;6(1):159-172
• All: 2 reference(s)