Cab39l^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cab39l^{tm1d(EUCOMM)Wtsi}
• Name: calcium binding protein 39-like; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7665327
• Gene: Cab39l Location: Chr14:59678400-59786353 bp, + strand Genetic Position: Chr14, 31.48 cM
• Alliance: Cab39l^{tm1d(EUCOMM)Wtsi} page

Mutation origin

• Mutant Cell Line: EPD0330_6_B12
• Germline Transmission: Earliest citation of germline transmission: J:350956
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 59743287 of Chromosome 14 upstream of the critical exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 6 at position 59744101. The critical exon 6 is thus flanked by loxP sites. Flp-medated recombination removed the lacZ and neomycin resistance gene. Exon 6 was deleted via cre-mediated recombination, generating a knockout mouse. (J:350956)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cab39l Mutation: 33 strains or lines available

References

• Original: J:350956 Lee S, et al., Loss of LPAR6 and CAB39L dysregulates the basal-to-luminal urothelial differentiation program, contributing to bladder carcinogenesis. Cell Rep. 2024 May 28;43(5):114146
• All: 1 reference(s)