Pabpc6^em1Sikas
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pabpc6^em1Sikas
• Name: poly(A) binding protein, cytoplasmic 6; endonuclease-mediated mutation 1, Shin-ichi Kashiwabara
• MGI ID: MGI:7666422
• Gene: Pabpc6 Location: Chr17:9885426-9888633 bp, - strand Genetic Position: Chr17, 7.47 cM
• Alliance: Pabpc6^em1Sikas page

Mutation origin

• Strain of Origin: ICR

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 188 bp deletion that deleted the region encoding a part of RNA-recognition motifs 1 and 2, which is involved in binding to poly(A) and EIF4G, and resulted in a premature termination codon due to a frame-shift mutation. Immunoblot analysis of testicular extracts confirmed absence of protein in homozygotes. (J:350818)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pabpc6 Mutation: 28 strains or lines available

References

• Original: J:350818 Kaku Y, et al., Intronless Pabpc6 encodes a testis-specific, cytoplasmic poly(A)-binding protein but is dispensable for spermatogenesis in the mouse. Biol Reprod. 2024 Apr 11;110(4):834-847
• All: 1 reference(s)