Col1a1^{tm1(Rr496-lacZ)Andg}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(Rr496-lacZ)Andg}
• Name: collagen, type I, alpha 1; targeted mutation 1, Guillaume Andrey
• MGI ID: MGI:7666522
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(Rr496-lacZ)Andg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:268374
• Parent Cell Line: C2 (Jaenisch) (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: ES cells containing the Col1a1^{tm13(neo/hygro*)Jae} allele were targeted, using Flp recombinase, with a vector to replace the FRT site flanked neomycin resistance gene cassette in the homing cassette downstream of Col1a1 with an FRT site flanked cassette containing Pitx1 promoter sequence followed by the beta-galactosidase reporter gene lacZ, PGK promoter sequence and ATG start site. (J:268374)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

References

• Original: J:268374 Kragesteen BK, et al., Dynamic 3D chromatin architecture contributes to enhancer specificity and limb morphogenesis. Nat Genet. 2018 Oct;50(10):1463-1473
• All: 1 reference(s)