Col1a1^{tm6(Rr284323-lacZ)Andg}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm6(Rr284323-lacZ)Andg}
• Name: collagen, type I, alpha 1; targeted mutation 6, Guillaume Andrey
• MGI ID: MGI:7666562
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm6(Rr284323-lacZ)Andg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:268374
• Parent Cell Line: C2 (Jaenisch) (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: ES cells containing the Col1a1^{tm13(neo/hygro*)Jae} allele were targeted, using Flp recombinase, with a vector to replace the FRT site flanked neomycin resistance gene cassette in the homing cassette downstream of Col1a1 with an FRT site flanked cassette containing putative Pitx1 enhancer sequence B (GRCm39:chr13:56258927-56261815 in intron 2 of Macroh2a1) followed by the beta-galactosidase reporter gene lacZ, PGK promoter sequence and ATG start site. (J:268374)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

References

• Original: J:268374 Kragesteen BK, et al., Dynamic 3D chromatin architecture contributes to enhancer specificity and limb morphogenesis. Nat Genet. 2018 Oct;50(10):1463-1473
• All: 1 reference(s)