Dcaf11^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dcaf11^{tm1d(KOMP)Wtsi}
• Name: DDB1 and CUL4 associated factor 11; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7703157
• Synonyms: Wdr23KO, Wdr23tm1d
• Gene: Dcaf11 Location: Chr14:55797463-55807522 bp, + strand Genetic Position: Chr14, 28.19 cM
• Alliance: Dcaf11^{tm1d(KOMP)Wtsi} page
• IMPC: Dcaf11 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:345927
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 55799862 of Chromosome 14 upstream of the critical exons 3- 11 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 3-11 at position 55803501. The critical exons 3-11 are thus flanked by loxP sites. The lacZ and neomycin resistance gene were removed via flp-mediated recombination. Exons 3-11 were deleted via cre-mediated recombination in the germline. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:345927, J:351189)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dcaf11 Mutation: 32 strains or lines available

References

• Original: J:345927 Liu J, et al., WDR23 mediates NRF2 proteostasis and cytoprotective capacity in the hippocampus. Mech Ageing Dev. 2024 Feb 1;218:111914
• All: 3 reference(s)