Anapc4^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Anapc4^{tm1c(EUCOMM)Wtsi}
• Name: anaphase promoting complex subunit 4; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7709701
• Gene: Anapc4 Location: Chr5:52991477-53024076 bp, + strand Genetic Position: Chr5, 28.81 cM, cytoband C1
• Alliance: Anapc4^{tm1c(EUCOMM)Wtsi} page
• IMPC: Anapc4 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:350417
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 52996543 of Chromosome 5 upstream of the exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 3 at position 52997349. The critical exon is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:173534, J:350417)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Anapc4 Mutation: 50 strains or lines available

References

• Original: J:350417 Day JL, et al., Deletion of a core APC/C component reveals APC/C function in regulating neuronal USP1 levels and morphology. Front Mol Neurosci. 2024;17:1352782
• All: 2 reference(s)