Igs7^{tm223(tetO-DhaA*,CAG-mScarlet,-tTA2)Hze}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm223(tetO-DhaA*,CAG-mScarlet,-tTA2)Hze}
• Name: intergenic site 7; targeted mutation 223, Hongkui Zeng
• MGI ID: MGI:7712098
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm223(tetO-DhaA*,CAG-mScarlet,-tTA2)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inducible, Inserted expressed sequence, RMCE-ready, Reporter, Transactivator)
• Mutation: Insertion
• Mutation details: The replacement vector designed with (from 5' to 3') an Bxb1 attB site, a partial green fluorescent protein sequence (non-functional 500bp fragment to increase the space between the Bxb1 attB site and the insulator sequence to improve stability of the targeting vector), two copies of the chicken beta-globin HS4 insulator element, a Tet response element/promoter (tetO), a loxP-STOP-loxP cassette, a farnesylated interchangeable ligand fusion protein (HaloTag; derived from Promega technologies) a woodchuck post-transcriptional regulatory element to increase mRNA stability (WPRE), a bovine growth hormone polyA (bGHpA), two copies of the chicken beta-globin HS4 insulator element, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an frt-flanked STOP cassette, a red fluorescent protein (mScarlet), a histone 2B (H2B), porcine teschovirus-1 2A (P2A), self-cleaving peptide, modified C22 tetracycline transactivator version 2 (tTA2), another WPRE sequence, another bGHpA sequence, PhiC31 AttB site, phosphoglycerate kinase 1 promoter (PGK), one domain of the hygromycin resistance gene, a splice domain and a mRNA splice acceptor sequence, a second domain of the hygromycin resistance gene, a SV40 pA sequence, and a PhiC31 AttP site was inserted into the T1 TIGRE locus (an intergenic region on chromosome 9 [between AB124611 and Carm1] determined to have low basal transcriptional activity and allow high Cre and/or Tet inducibility). (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 66 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)