Igs7^{tm163.1(tetO-GCaMP6s,CAG-tdTomato,-tTA2)Hze}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm163.1(tetO-GCaMP6s,CAG-tdTomato,-tTA2)Hze}
• Name: intergenic site 7; targeted mutation 163.1, Hongkui Zeng
• MGI ID: MGI:7712112
• Synonyms: Ai163D
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm163.1(tetO-GCaMP6s,CAG-tdTomato,-tTA2)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Inserted expressed sequence, RMCE-ready, Reporter, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The pTRE2-LSL-GCaMP6s-CAG-LSL-tdTom-tTA2 replacement vector designed with (from 5' to 3') an frt3 site, two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a Tet response element/promoter (TRE2), a loxP-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), a GCaMP6 slow variant calcium indicator sequence (GCaMP6s), a WPRE (to enhance the mRNA transcript stability), a BGH polyA, two copies of chicken beta-globin HS4 insulator element, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from pCAGGS), a lox2272-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-TKpA), tdTomato sequence, a porcine teschovirus-1 2A, self-cleaving peptide (P2A), a synthetic modified tetracycline-regulated transactivator gene (tTA2(S)), a WPRE, a BGH polyA, an AttB site, a PGK-5'hygro cassette, an RNA splice donor and a frt5 site was inserted into the T1 TIGRE locus (an intergenic region on chromosome 9 [between AB124611 and Carm1] determined to have low basal transcriptional activity and allow high Cre and/or Tet inducibility). PhiC31 recombinase removed the AttB/AttP-flanked PGK-hygro-SV40polyA cassette and replaced it with the recombined AttB/AttP site (AttL). (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 66 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)