Mir582^em1Minz
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mir582^em1Minz
• Name: microRNA 582; endonuclease-mediated mutation 1, Min-Hua Zheng
• MGI ID: MGI:7712843
• Gene: Mir582 Location: Chr13:109461278-109461358 bp, + strand Genetic Position: Chr13, 59.87 cM
• Alliance: Mir582^em1Minz page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 1,034 bp deletion by knocking-out Mir582 in the intron between exons 3 and 4 of the host gene Pde4d using sgRNAs 5'-CGTGCAGAGGACATATACACAGG and 5'-TGGTACACGGGATTAAGCTCAGG. While expression of Mir582 is absent in the brain, Pde4d expression is unaffected. (J:325358)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir582 Mutation: 1 strain or line available

References

• Original: J:325358 Zhang YF, et al., MicroRNA-582-5p Contributes to the Maintenance of Neural Stem Cells Through Inhibiting Secretory Protein FAM19A1. Front Cell Neurosci. 2022;16:866020
• All: 1 reference(s)