Tnc^{tm1.1(cre/ERT2)Gcr}
Targeted Allele Detail

Summary

• Symbol: Tnc^{tm1.1(cre/ERT2)Gcr}
• Name: tenascin C; targeted mutation 1, Gerhard Christofori
• MGI ID: MGI:7712998
• Gene: Tnc Location: Chr4:63878022-63965252 bp, - strand Genetic Position: Chr4, 34.06 cM
• Alliance: Tnc^{tm1.1(cre/ERT2)Gcr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tnc^{tm1.1(cre/ERT2)Gcr} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tnc (MGI:101922)

• Mutation details: The targeting vector was designed to insert a Cre/ERT2 fusion protein into the tenascin C (Tnc) locus. Specifically, this vector includes (from 5' to 3') an FRT-flanked Neomycin-STOP, a cre/ERT2 fusion gene (Cre recombinase fused to a human estrogen receptor ligand binding domain) and rabbit beta-globin gene polyadenylation signal. The construct was electroporated into (C57BL/6NTac x 129S6/SvEvTac)F1-derived iTL BA1 embryonic stem (ES) cells and correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric mice were identified by genotyping and bred to a C57BL/6 FLP mouse line to remove the neomycin cassette. (J:101977, J:316041)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tnc (mouse) Summary of all recombinase alleles driven by Tnc. Alliance mouse Tnc gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tnc Mutation: 135 strains or lines available

References

• Original: J:316041 Luond F, et al., Distinct contributions of partial and full EMT to breast cancer malignancy. Dev Cell. 2021 Nov 23
• All: 2 reference(s)