Ube2f^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ube2f^{tm1c(EUCOMM)Hmgu}
• Name: ubiquitin-conjugating enzyme E2F (putative); targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7738243
• Gene: Ube2f Location: Chr1:91177412-91214243 bp, + strand Genetic Position: Chr1, 46.08 cM
• Alliance: Ube2f^{tm1c(EUCOMM)Hmgu} page
• IMPC: Ube2f gene page

Mutation origin

• Mutant Cell Line: HEPD0820_4_G09
• Germline Transmission: Earliest citation of germline transmission: J:342795
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 91202481 of Chromosome 1 upstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon at position 91203328. The critical exon is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065, J:348744)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ube2f Mutation: 18 strains or lines available

References

• Original: J:342795 Wu D, et al., The Ube2m-Rbx1 neddylation-Cullin-RING-Ligase proteins are essential for the maintenance of Regulatory T cell fitness. Nat Commun. 2022 May 31;13(1):3021
• All: 3 reference(s)