Tex44^em1Sbbx
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tex44^em1Sbbx
• Name: testis expressed 44; endonuclease-mediated mutation 1, Sandrine Barbaux
• MGI ID: MGI:7738738
• Synonyms: Tex44-KO
• Gene: Tex44 Location: Chr1:86354051-86355771 bp, + strand Genetic Position: Chr1, 43.94 cM, cytoband C5
• Alliance: Tex44^em1Sbbx page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing technology was used to generate a deletion of the complete open reading frame included in the unique exon of the gene. RT-PCR showed loss of Tex44 mRNA in testis samples from homozygous mutant males. Additional internal PCR performed on genomic DNA confirmed the complete absence of the gene. (J:354514)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tex44 Mutation: 19 strains or lines available

References

• Original: J:354514 Dupuis S, et al., The lack of Tex44 causes severe subfertility with flagellar abnormalities in male mice. Cell Mol Biol Lett. 2024 May 15;29(1):74
• All: 1 reference(s)