Tmem225^em1Jliu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tmem225^em1Jliu
• Name: transmembrane protein 225; endonuclease-mediated mutation 1, Jiali Liu
• MGI ID: MGI:7738841
• Synonyms: Tmem225^-, Tmem225 KO
• Gene: Tmem225 Location: Chr9:40059418-40062170 bp, + strand Genetic Position: Chr9, 21.42 cM, cytoband B
• Alliance: Tmem225^em1Jliu page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing technology was used to generate a deletion of exons 2, 3 and part of 4 of the gene. Western blot analysis confirmed the absence of protein expression in the testes and spermatozoa of homozygous mutant male mice. (J:345843)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmem225 Mutation: 13 strains or lines available

References

• Original: J:345843 Lv Z, et al., TMEM225 Is Essential for Sperm Maturation and Male Fertility by Modifying Protein Distribution of Sperm in Mice. Mol Cell Proteomics. 2024 Feb;23(2):100720
• All: 1 reference(s)