Gt(ROSA)26Sor^{em5(CAG-KANK4,-tdTomato)Bcgen}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em5(CAG-KANK4,-tdTomato)Bcgen}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 5, Biocytogen LLC
• MGI ID: MGI:7748772
• Synonyms: Rosa26-LSL-KANK4-tdTomato
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em5(CAG-KANK4,-tdTomato)Bcgen} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 technology inserted a CAG promoter, loxP-flanked transcriptional Stop element, flag tagged human KANK4 coding sequence, P2A, tdTomato, and WPRE were inserted into the locus. This creates a conditional-ready allele that will express the human gene and reporter only after cre-mediated excision of the STOP cassette. (J:326312)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1002 strains or lines available

References

• Original: J:326312 Zhang C, et al., KANK4 Promotes Arteriogenesis by Potentiating VEGFR2 Signaling in a TALIN-1-Dependent Manner. Arterioscler Thromb Vasc Biol. 2022 Jun;42(6):772-788
• All: 1 reference(s)