Pramel15^em2Bzhu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pramel15^em2Bzhu
• Name: PRAME like 15; endonuclease-mediated mutation 2, Bing Zhu
• MGI ID: MGI:7788863
• Gene: Pramel15 Location: Chr4:144099330-144104503 bp, - strand Genetic Position: Chr4, 77.98 cM
• Alliance: Pramel15^em2Bzhu page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology using gRNA 5-CTGTTGAAGCAAGCCTACGA-3 targeting the first exon generated a 14-nucleotide deletion resulting in a frameshift and a premature stop codon. This allele corresponds to line No. 28. (J:354132)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pramel15 Mutation: 23 strains or lines available

References

• Original: J:354132 Tan J, et al., Pramel15 facilitates zygotic nuclear DNMT1 degradation and DNA demethylation. Nat Commun. 2024 Aug 25;15(1):7310
• All: 1 reference(s)