Med15^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Med15^{tm1c(KOMP)Wtsi}
• Name: mediator complex subunit 15; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7788880
• Synonyms: Med15^fl
• Gene: Med15 Location: Chr16:17469072-17540811 bp, - strand Genetic Position: Chr16, 10.94 cM
• Alliance: Med15^{tm1c(KOMP)Wtsi} page
• IMPC: Med15 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:355262
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 17490712 of Chromosome 16 upstream of the critical exons 6 and 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exons 6 and 7 at position 17492958. Exons 6 and 7 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:355262)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Med15 Mutation: 50 strains or lines available

References

• Original: J:355262 Kadhim AZ, et al., Transcriptional coactivator MED15 is required for beta cell maturation. Nat Commun. 2024 Oct 8;15(1):8711
• All: 1 reference(s)