Rtf2tm1c(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Rtf2tm1c(KOMP)Wtsi |
Name: |
replication termination factor 2; targeted mutation 1c, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:7852632 |
Synonyms: |
Rtf2lox |
Gene: |
Rtf2 Location: Chr2:172282498-172311819 bp, + strand Genetic Position: Chr2, 94.86 cM, cytoband H3
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Alliance: |
Rtf2tm1c(KOMP)Wtsi page
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IMPC: |
Rtf2 gene page |
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Mutant Cell Line: |
EPD0137_6_E01 |
Germline Transmission: |
Earliest citation of germline transmission:
J:360903
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Parent Cell Line: |
JM8.N4 (ES Cell)
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Strain of Origin: |
C57BL/6N
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Conditional ready) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 172286890 of Chromosome 2 upstream of the critical exon, exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 172287588. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:173534, J:360903)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Rtf2 Mutation: |
17 strains or lines available
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Original: |
J:360903 Conti BA, et al., RTF2 controls replication repriming and ribonucleotide excision at the replisome. Nat Commun. 2024 Mar 2;15(1):1943 |
All: |
2 reference(s) |
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