Rr597^em1Yagk
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rr597^em1Yagk
• Name: regulatory region 597; endonuclease-mediated mutation 1, Yana G Kamberov
• MGI ID: MGI:7855976
• Synonyms: mECE20^KO
• Gene: Rr597 Location: unknown Genetic Position: Chr1, Syntenic
• Alliance: Rr597^em1Yagk page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region)
• Mutation: Intragenic deletion
• Mutation details: The En1 enhancer, located downstream in an intron of Celrr, was targeted using sgRNAs (equivalent to AAGGAACCACATCGCAAAGAGGG and CCGGGCCTCGGCCACTACTCGGG) with CRISPR/Cas9 technology, resulting in a 1674 bp deletion (GRCm39:chr1:121104405-121106078). Expression of En1 is severely reduced in mid-hindbrain and moderately reduced in palm (volar) skin. (J:333368)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr597 Mutation: 0 strains or lines available

References

• Original: J:333368 Aldea D, et al., Differential modularity of the mammalian Engrailed 1 enhancer network directs sweat gland development. PLoS Genet. 2023 Feb;19(2):e1010614
• All: 2 reference(s)