Cst7^tm1.2Arte
Targeted Allele Detail

Summary

• Symbol: Cst7^tm1.2Arte
• Name: cystatin F (leukocystatin); targeted mutation 1.1, TaconicArtemis
• MGI ID: MGI:7859338
• Gene: Cst7 Location: Chr2:150412335-150420864 bp, + strand Genetic Position: Chr2, 74.67 cM, cytoband G1-G3
• Alliance: Cst7^tm1.2Arte page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:258944
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A construct containing a loxP site, FRT-flanked pgk-neo cassette, exon 2 containing two amino acid substitutions (C25A, C62A), and second loxP site was inserted in place of the endogenous exon 2 via homologous recombination. Recombinase-mediated recombination removed the neo cassette and exon 2. Immunoblot analysis confirmed the absence of protein expression in homozygous mice. (J:258944)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cst7 Mutation: 18 strains or lines available

References

• Original: J:258944 Matthews SP, et al., Cystatin F Ensures Eosinophil Survival by Regulating Granule Biogenesis. Immunity. 2016 Apr 19;44(4):795-806
• All: 2 reference(s)