Map4^em2Coop
Endonuclease-mediated Allele Detail

Summary

• Symbol: Map4^em2Coop
• Name: microtubule-associated protein 4; endonuclease-mediated mutation 2, Thomas A Cooper
• MGI ID: MGI:7865836
• Gene: Map4 Location: Chr9:109760528-109913023 bp, + strand Genetic Position: Chr9, 59.83 cM
• Alliance: Map4^em2Coop page

Mutation origin

• Strain of Origin: FVB/NJ

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/cas9 mediated recombination using guide RNAs removed exon 3 resulting in a frameshift and premature stop mutation. Western blot analysis confirmed the absence of expression in striated muscles from homozygous mice. (J:358165)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map4 Mutation: 230 strains or lines available

References

• Original: J:358165 Lucas L, et al., Alternatively spliced MAP4 isoforms have key roles in maintaining microtubule organization and skeletal muscle function. iScience. 2024 Nov 15;27(11):111104
• All: 1 reference(s)