Spi1^{tm1(EGFP/cre/ERT2)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Spi1^{tm1(EGFP/cre/ERT2)Wtsi}
• Name: Spi-1 proto-oncogene; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:7867268
• Synonyms: Spi1^{tm1(EGFP_CreERT2)Wtsi}
• Gene: Spi1 Location: Chr2:90912750-90946104 bp, + strand Genetic Position: Chr2, 50.44 cM, cytoband E3
• Alliance: Spi1^{tm1(EGFP/cre/ERT2)Wtsi} page

Mutation origin

• Mutant Cell Line: CEPD0033_2_A04
• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Null/knockout, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Spi1^{tm1(EGFP/cre/ERT2)Wtsi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Spi1 (MGI:98282)

• Mutation details: The following elements were inserted into intron 3: FRT site, En2 splice acceptor sequence, sequence coding for F2A self-cleaving peptide, EGFP reporter gene, sequence coding for T2A self-cleaving peptide, cre/ERT2 inducible recombinase gene, SV40 poly(A) signal sequence, Rox site flanked puromycin resistance gene cassette, and a loxP site. A second loxP site was inserted into intron 4. This creates a reporter-recombinase knockout allele with a floxed exon 4. (J:82809)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Spi1 (mouse) Summary of all recombinase alleles driven by Spi1. Alliance mouse Spi1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spi1 Mutation: 28 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)