Mir204^tm1.2Dic
Targeted Allele Detail

Summary

• Symbol: Mir204^tm1.2Dic
• Name: microRNA 204; targeted mutation 1.2, Di Chen
• MGI ID: MGI:7868148
• Gene: Mir204 Location: Chr19:22727969-22728036 bp, + strand Genetic Position: Chr19, 17.26 cM
• Alliance: Mir204^tm1.2Dic page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:276908
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: An IRES-lacZ reporter cassette as well as a loxP site were inserted 178 bp upstream miR-204 stem loop and a loxP site plus a neomycin selection cassette were placed 260 bp downstream miRNA stem loops by homologous recombination in ES cells. The chimeric mice were crossed to flippase-expressing transgenic mice to remove Frt or F3 (a mutant of Frt) flanked lacZ and Neo cassettes, leaving two loxP sites immediately flanking the miRNA stem-loops. Mice were further crossed to CMV-cre mice, resulting in the deletion of loxP-flanked sequences. (J:276908)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir204 Mutation: 5 strains or lines available

References

• Original: J:276908 Huang J, et al., The microRNAs miR-204 and miR-211 maintain joint homeostasis and protect against osteoarthritis progression. Nat Commun. 2019 Jun 28;10(1):2876
• All: 2 reference(s)