Dnm1l^em1Bay
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dnm1l^em1Bay
• Name: dynamin 1-like; endonuclease-mediated mutation 1, Baylor College of Medicine
• MGI ID: MGI:8158798
• Gene: Dnm1l Location: Chr16:16130094-16176823 bp, - strand Genetic Position: Chr16, 10.12 cM
• Alliance: Dnm1l^em1Bay page

Mutation origin

• Strain of Origin: C57BL/6J
• Project Collection: CPMM

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Humanized sequence)
• Mutation: Insertion
• Mutation details: Modeling human variant NM_012062.5: c.1207C>T; p.R403C using a conditional by inversion (COIN) allele design. Exons 10, 11, and 12, were duplicated and inserted in an antisense orientation upstream of the endogenous exon 10 in Dnm1l (NM_001276340.2). A Lox66 site was inserted upstream of the antisense exon 10 and a reverse complement Lox71 site was inserted downstream of the endogenous exon 12. A Lox511 site was inserted upstream of the Lox66 site and a reverse complement Lox511 site was inserted downstream of the antisense exon 12. With cre-mediated recombination, the antisense exons are moved into the sense orientation for expression of the variant-containing exon and the endogenous exons are recombined out of the allele. (J:358610)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnm1l Mutation: 44 strains or lines available

References

• Original: J:358610 Baylor College of Medicine, Baylor College of Medicine Alleles. MGI Direct Data Submission. 2024
• All: 1 reference(s)