Phenotypes associated with this allele

• Allele Symbol: Nodal^{TgR(MPSVNeo)413.dRob}
• Allele Name: retroviral transgene insertion, Elizabeth J Robertson
• Allele ID: MGI:1856970
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nodal^TgR(MPSVNeo)413.dRob/Nodal^TgR(MPSVNeo)413.dRob	129S/SvEv-Nodal^TgR(MPSVNeo)413.dRob	MGI:2177171
ht2	Nodal^TgR(MPSVNeo)413.dRob/Nodal^tm1Mku	involves: 129S1/Sv	MGI:3850216
ht3	Nodal^TgR(MPSVNeo)413.dRob/Nodal^tm4Rob	involves: 129S/SvEv * C57BL/6	MGI:2668420
ht4	Nodal^TgR(MPSVNeo)413.dRob/Nodal^tm6Rob	involves: 129S/SvEv * C57BL/6	MGI:3521752

Genotype
MGI:2177171

hm1

• Allelic Composition: Nodal^{TgR(MPSVNeo)413.dRob}/Nodal^{TgR(MPSVNeo)413.dRob}
• Genetic Background: 129S/SvEv-Nodal^{TgR(MPSVNeo)413.dRob}

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

prenatal lethality, complete penetrance ( J:11389 )

• no live born homozygotes are detected

embryo

abnormal developmental patterning ( J:11389 )

• no formation of the embryonic axis, head-fold or somites

abnormal ectoderm development ( J:19211 )

• disorganized expansion of the embryonic ectoderm

abnormal mesoderm development ( J:3969 )

embryonic growth arrest ( J:19211 )

• development is arrested shortly after gastrulation

decreased embryo size ( J:11389 )

abnormal embryonic tissue morphology ( J:11389 , J:19211 )

• at E8.5, no fetal portion evident and at E9.5, embryo is highly disorganized (J:11389)

absent head fold ( J:11389 )

• no recognizable head fold formation

• at E8.5, the region normally occupied by early-head-fold-stage embryo contained randomly organized, highly folded multicellular tissue layers inserted into the ectoplacental cone

failure of primitive streak formation ( J:19211 )

• at an early gastrulation stage (E6.5) no evidence of the primitive streak

absent somites ( J:11389 )

abnormal extraembryonic tissue morphology ( J:19211 )

absent allantois ( J:11389 )

absent amnion ( J:11389 )

absent amniotic folds ( J:19211 )

abnormal extraembryonic ectoderm morphology ( J:19211 )

• the extra-embryonic ectoderm is not displaced proximally towards the ectoplacental cone by mesodermal cells

• disorganized expansion of the extra-embryonic ectoderm

• the proximal region of the embryo, comprising the extra-embryonic ectoderm, was no longer symmetrical but often exhibited an irregular twisted surface

abnormal parietal yolk sac morphology ( J:11389 )

• the correct number of parietal endoderm cells form, but the parietal yolk sac fails to expand correctly in the absence of a normal, rapidly growing, fetal portion

increased trophoblast giant cell number ( J:70738 )

small ectoplacental cone ( J:11389 )

• at E9.5, ectoplacental cone reduced in size

abnormal spongiotrophoblast layer morphology ( J:70738 )

• diploid spongiotrophoblasts are lost

absent placental labyrinth ( J:70738 )

absent visceral yolk sac ( J:11389 )

• absent visceral yolk sac at E8.5

abnormal extraembryonic mesoderm development ( J:19211 )

• deficient formation and/or movement of the extra-embryonic mesoderm

growth/size/body

decreased embryo size ( J:11389 )

Genotype
MGI:3850216

ht2

• Allelic Composition: Nodal^{TgR(MPSVNeo)413.dRob}/Nodal^tm1Mku
• Genetic Background: involves: 129S1/Sv

embryo

increased trophoblast giant cell number ( J:70738 )

• expanded giant cell layer

increased spongiotrophoblast size ( J:70738 )

• cell layer is expanded in thickness

thin placenta labyrinth ( J:70738 )

• reduced in thickness

Genotype
MGI:2668420

ht3

• Allelic Composition: Nodal^{TgR(MPSVNeo)413.dRob}/Nodal^tm4Rob
• Genetic Background: involves: 129S/SvEv * C57BL/6

mortality/aging

embryonic lethality between somite formation and embryo turning, complete penetrance ( J:84300 )

• embryos develop severe anterior truncations at E8.5

embryo

rostral body truncation ( J:84300 )

• embryos develop severe anterior truncations at E8.5

abnormal embryonic neuroepithelium morphology ( J:84300 )

• at E9.5, anterior neurectoderm fails to proliferate and is filled with pyknotic nuclei

cardiovascular system

abnormal heart looping ( J:84300 )

• heart tube is abnormally looped

pericardial edema ( J:84300 )

• pericardium is often edematous

enlarged pericardium ( J:84300 )

• pericardium is often enlarged

digestive/alimentary system

abnormal foregut morphology ( J:84300 )

• gut tube forms but is poorly elaborated in anterior region; instead of extending to level of Rathke's pouch like wild-type, no gut tissue is evident at this level in mutants and is absent anterior to level of heart

homeostasis/metabolism

pericardial edema ( J:84300 )

• pericardium is often edematous

nervous system

abnormal embryonic neuroepithelium morphology ( J:84300 )

• at E9.5, anterior neurectoderm fails to proliferate and is filled with pyknotic nuclei

absent midbrain ( J:84300 )

• midbrain regions are not specified

absent forebrain ( J:84300 )

• forebrain regions are not specified

decreased embryonic neuroepithelial cell proliferation ( J:84300 )

• the anterior neurectoderm fails to proliferate

Genotype
MGI:3521752

ht4

• Allelic Composition: Nodal^{TgR(MPSVNeo)413.dRob}/Nodal^tm6Rob
• Genetic Background: involves: 129S/SvEv * C57BL/6

cardiovascular system

trabecula carnea hypoplasia ( J:94418 )

• the density of trabeculae is decreased compared to controls in 4/12 mutants

thin myocardium compact layer ( J:94418 )

• in 4/12 mutants the compact myocardial layer is thinner

abnormal direction of heart looping ( J:94418 )

• heart looping is reversed in less than 2% of mutants

delayed heart development ( J:94418 )

• cardiac morphogenesis is delayed

common atrium ( J:94418 )

• a common atrium is seen in 3/12 mutants at E13.5

abnormal interventricular septum morphology ( J:94418 )

• at E13.5 ventricular septal wall formation is incomplete in about 42% of mutants

growth/size/body

right pulmonary isomerism ( J:94418 )

• partial right isomerism is seen in 4% of mutant embryos

muscle

trabecula carnea hypoplasia ( J:94418 )

• the density of trabeculae is decreased compared to controls in 4/12 mutants

thin myocardium compact layer ( J:94418 )

• in 4/12 mutants the compact myocardial layer is thinner

respiratory system

right pulmonary isomerism ( J:94418 )

• partial right isomerism is seen in 4% of mutant embryos