Phenotypes associated with this allele

• Allele Symbol: Rab23^opb
• Allele Name: open brain
• Allele ID: MGI:1856980
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rab23^opb/Rab23^opb	B6.Cg-Rab23^opb	MGI:4358379
hm2	Rab23^opb/Rab23^opb	involves: C57BL/6 * NMRI	MGI:2168958

Genotype
MGI:4358379

hm1

• Allelic Composition: Rab23^opb/Rab23^opb
• Genetic Background: B6.Cg-Rab23^opb

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Rab23^opb/Rab23^opb embryos display neural tube closure defects, missing eyes, preaxial duplications of limbs, and detached skin

limbs/digits/tail

abnormal forelimb zeugopod morphology ( J:42171 )

• at E15 preaxial duplications are seen in alcian blue and alizarin stained skeletal preparations

abnormal hindlimb zeugopod morphology ( J:42171 )

• at E15 preaxial duplications are seen in alcian blue and alizarin stained skeletal preparations

nervous system

abnormal neural tube morphology ( J:42171 )

• most embryos have malformed, dorsally protruding but closed neural tube in varying trunk regions

• severe open neural tube malformations result in spina bifida

open neural tube ( J:42171 )

• 1.1 % of E10.5 - E12.5 embryos exhibit an open neural tube at the lumbosacral region; this will result in severe spina bifida

spina bifida ( J:42171 )

spina bifida occulta ( J:42171 )

• at E15 several embryos exhibited spinal cords open dorsally without being covered by skin in the lumbosacral axial regions

exencephaly ( J:42171 )

• Background Sensitivity: 2.3% of mutant embryos on a C57BL/6 congenic background are exencephalic; all mutant embryos on a mixed C57BL/6 and NMRI have exencephaly

abnormal spinal cord morphology ( J:42171 )

• at E15 the the spinal cord is open dorsally in the lumbo-sacral or thoracolumbar axial regions

skeleton

abnormal vertebral arch development ( J:42171 )

• at E13.5 - E19.5 the neural arches are wide open dorsally in trunk regions compared with wild-type fetuses

spina bifida occulta ( J:42171 )

• at E15 several embryos exhibited spinal cords open dorsally without being covered by skin in the lumbosacral axial regions

vision/eye

anophthalmia ( J:42171 )

• detected at E15

embryo

abnormal neural tube morphology ( J:42171 )

• most embryos have malformed, dorsally protruding but closed neural tube in varying trunk regions

• severe open neural tube malformations result in spina bifida

open neural tube ( J:42171 )

• 1.1 % of E10.5 - E12.5 embryos exhibit an open neural tube at the lumbosacral region; this will result in severe spina bifida

spina bifida ( J:42171 )

spina bifida occulta ( J:42171 )

• at E15 several embryos exhibited spinal cords open dorsally without being covered by skin in the lumbosacral axial regions

integument

skin detachment ( J:42171 )

• at E15 the skin of the rostral body is detached dorsally

Genotype
MGI:2168958

hm2

• Allelic Composition: Rab23^opb/Rab23^opb
• Genetic Background: involves: C57BL/6 * NMRI

nervous system

abnormal neural tube closure ( J:21617 )

open neural tube ( J:21617 )

• Background Sensitivity: the frequency of open tube phenotype decreased with increasing C57BL/6 background

abnormal neural tube ventricular layer morphology ( J:21617 )

• extensive necrosis

abnormal postnatal subventricular zone morphology ( J:21617 )

• extensive necrosis

exencephaly ( J:21617 , J:42171 )

• at day E10.5 this condition extends from the forebrain into the rhombencephalon rostral to the otic vesicle revealing Rathke's pouch and the infundibulum (J:21617)

• Background Sensitivity: 100% of mutant mice on this mixed background have exencephaly; 2.3% of mutant embryos having a C57BL/6 congenic background are exencephalic (J:42171)

abnormal dorsal root ganglion morphology ( J:21617 )

• severe malformations in the thoracic region

abnormal spinal cord morphology ( J:21617 )

• severe malformations in the thoracic region

• exhibit a circular to oval shape

• defects are seen in both ventral and dorsal regions of the cord

abnormal spinal cord central canal morphology ( J:21617 )

• enlarged

vision/eye

abnormal lens induction ( J:21617 )

• there is no induction

abnormal lens vesicle development ( J:21617 )

• at E12.5 no induction of lens vesicle is evident in many mutants

abnormal optic cup morphology ( J:21617 )

• abnormal flattened appearance is evident

abnormal retina neuronal layer morphology ( J:21617 )

• this layer does not form

skeleton

abnormal tarsal bone morphology ( J:21617 )

• duplicated distal tarsus bone

absent tibia ( J:21617 )

abnormal axial skeleton morphology ( J:21617 )

limbs/digits/tail

abnormal tarsal bone morphology ( J:21617 )

• duplicated distal tarsus bone

abnormal hindlimb zeugopod morphology ( J:21617 )

absent tibia ( J:21617 )

abnormal limb development ( J:21617 )

embryo

abnormal neural tube closure ( J:21617 )

open neural tube ( J:21617 )

• Background Sensitivity: the frequency of open tube phenotype decreased with increasing C57BL/6 background

abnormal neural tube ventricular layer morphology ( J:21617 )

• extensive necrosis