Phenotypes associated with this allele

• Allele Symbol: Ace^tm1Unc
• Allele Name: targeted mutation 1, University of North Carolina
• Allele ID: MGI:1857115
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ace^tm1Unc/Ace^tm1Unc	involves: 129P2/OlaHsd	MGI:6402546
hm2	Ace^tm1Unc/Ace^tm1Unc	involves: 129P2/OlaHsd * C57BL/6J	MGI:2450563
ht3	Ace^tm1Unc/Ace^+	B6.129P2-Ace^tm1Unc	MGI:3799538
ht4	Ace^tm1Unc/Ace^+	involves: 129P2/OlaHsd	MGI:3799540
ht5	Ace^tm1Unc/Ace^+	involves: 129P2/OlaHsd * C57BL/6J	MGI:2450564
cx6	Ace^tm1Unc/Ace^tm1Unc Ace2^tm1Pngr/Ace2^tm1Pngr	involves: 129P2/OlaHsd	MGI:2661736
cx7	Ace^tm1Unc/Ace^tm1Unc Ace2^tm1Pngr/Y	involves: 129P2/OlaHsd	MGI:2661733

Genotype
MGI:6402546

hm1

• Allelic Composition: Ace^tm1Unc/Ace^tm1Unc
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

decreased angiotensin II level ( J:100334 )

• mice exposed to acid aspiration to induce acute lung injury show decreased angiotensin II levels in lung compared to single Ace2^tm1Pngr mutants

decreased circulating angiotensin II level ( J:100334 )

• mice exposed to acid aspiration to induce acute lung injury show decreased angiotensin II levels in plasma compared to single Ace2^tm1Pngr mutants

decreased susceptibility to injury ( J:100334 )

• mice are partly protected against acute lung injury induced by acid-aspiration

Genotype
MGI:2450563

hm2

• Allelic Composition: Ace^tm1Unc/Ace^tm1Unc
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

cellular

impaired sperm migration in female genital tract ( J:46177 )

♂ • very few mutant sperm progress beyond the two oviduct regions nearest the uterus at 1 hr after mating, whereas significantly more wild-type sperm reach the extramural uterotubal junction and the lower and upper isthmus regions of the oviducts under identical conditions

mortality/aging

preweaning lethality, incomplete penetrance ( J:25284 )

• intercrossing of heterozygotes results in about 11% homozygous mutant mice compared with the expected 25%; the cause of this deviation has not been determined

• at weaning, the proportions of wild-type, heterozygous and homozygous offspring differ significantly from the expected 1:2:1 Mendelian ratios

reproductive system

reproductive system phenotype ( J:25284 , J:46177 )

N • females are fertile (J:25284)

N • males show normal testes morphology, normal spermatogenesis, and normal sperm count and morphology (J:25284)

♂N • sperm show no significant differences in assays of viability, motility, capacitation, and induction of the acrosome reaction relative to wild-type sperm (J:46177)

impaired sperm migration in female genital tract ( J:46177 )

♂ • very few mutant sperm progress beyond the two oviduct regions nearest the uterus at 1 hr after mating, whereas significantly more wild-type sperm reach the extramural uterotubal junction and the lower and upper isthmus regions of the oviducts under identical conditions

decreased litter size ( J:25284 )

• 2 pups in a single litter

reduced male fertility ( J:25284 )

♂ • 80% (4 of 5) males are unable to sire offspring

♂ • reduced fertility putatively due to an impaired ability to fertilize ova

impaired fertilization ( J:46177 )

• mutant sperm show defects in transport within the oviducts and in binding to zonae pellucidae

• even those mutant sperm that reach the oviductal ampulla are less likely to fertilize eggs because of their reduced capacity for zona binding

decreased fertilization frequency ( J:46177 )

• sperm from male homozygotes achieve in vivo fertilization at a reduced frequency: >65% of eggs from wild-type matings become fertilized and develop to the 8-cell stage or beyond compared with <5% of eggs harvested from wild-type females inseminated by homozygous mutant males

impaired binding of sperm to zona pellucida ( J:46177 )

♂ • mutant sperm show defects in binding to zonae pellucidae

cardiovascular system

abnormal kidney vasculature morphology ( J:25284 )

• intrarenal arteries display significant mural hypercellularity and thickening with narrowing of the lumen

hypotension ( J:25284 )

• blood pressure is reduced by ~35 mm Hg in both males and females

renal/urinary system

abnormal kidney vasculature morphology ( J:25284 )

• intrarenal arteries display significant mural hypercellularity and thickening with narrowing of the lumen

kidney inflammation ( J:25284 )

• chronic inflammation

abnormal renal glomerulus morphology ( J:25284 )

• crowding of glomeruli

kidney cortex atrophy ( J:25284 )

• focal areas of cortical thinning

renal tubule atrophy ( J:25284 )

• shrinkage of tubules associated with a wedge-shaped zone of cortical atrophy in the subcapsular renal cortex

immune system

kidney inflammation ( J:25284 )

• chronic inflammation

Genotype
MGI:3799538

ht3

• Allelic Composition: Ace^tm1Unc/Ace⁺
• Genetic Background: B6.129P2-Ace^tm1Unc

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:72577 )

N • streptozotocin induces a diabetes phenotype indistinguishable from that observed in similarly treated wild-type mice

Genotype
MGI:3799540

ht4

• Allelic Composition: Ace^tm1Unc/Ace⁺
• Genetic Background: involves: 129P2/OlaHsd

cardiovascular system

increased heart weight ( J:42519 )

increased heart rate ( J:42519 )

growth/size/body

increased heart weight ( J:42519 )

Genotype
MGI:2450564

ht5

• Allelic Composition: Ace^tm1Unc/Ace⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

mortality/aging

postnatal lethality, incomplete penetrance ( J:25284 )

• at weaning, the proportions of wild-type and heterozygous offspring differ significantly from the expected Mendelian ratios; the cause of this deviation has not been determined

cardiovascular system

hypotension ( J:25284 )

♂ • blood pressure is reduced by 15-20 mm Hg in males

♂ • females display normal blood pressure

growth/size/body

increased body weight ( J:128859 )

• compared to in Ace^tm3Unc heterozygotes when fed a high fat diet

adipose tissue

abnormal epididymal fat pad morphology ( J:128859 )

• when fed a high fat diet, peri-epididymal adipose tissue is increased compared to in Ace^tm3Unc heterozygotes

reproductive system

reproductive system phenotype ( J:46177 )

♂N • males heterozygotes sire wild-type and heterozygous offspring at an indistinguishable frequency, indicating functionally intact sperm

endocrine/exocrine glands

increased insulin secretion ( J:128859 )

• compared to in Ace^tm3Unc heterozygotes when fed a high fat diet

homeostasis/metabolism

increased insulin secretion ( J:128859 )

• compared to in Ace^tm3Unc heterozygotes when fed a high fat diet

Genotype
MGI:2661736

cx6

• Allelic Composition: Ace^tm1Unc/Ace^tm1Unc; Ace2^tm1Pngr/Ace2^tm1Pngr
• Genetic Background: involves: 129P2/OlaHsd

cardiovascular system

hypotension ( J:77232 )

♀ • similar to Ace^tm1Unc homozygotes however normal cardiac contractility is seen unlike Ace^tm1Unc homozygotes

homeostasis/metabolism

decreased angiotensin II level ( J:100334 )

♀ • mice exposed to acid aspiration to induce acute lung injury show decreased angiotensin II levels in lung compared to single Ace2^tm1Pngr mutants

decreased circulating angiotensin II level ( J:100334 )

♀ • mice exposed to acid aspiration to induce acute lung injury show decreased angiotensin II levels in plasma compared to single Ace2^tm1Pngr mutants

decreased susceptibility to injury ( J:100334 )

♀ • mice show rescue of severe lung failure, edema formation, and histological lung changes induced by acid aspiration in single Ace2^tm1Pngr mutants

♀ • mice show rescue of the severe lung impairments seen in single Ace2^tm1Pngr mutants with endotoxin-induced acute lung injury

renal/urinary system

abnormal kidney morphology ( J:77232 )

♀ • similar to Ace^tm1Unc homozygotes

Genotype
MGI:2661733

cx7

• Allelic Composition: Ace^tm1Unc/Ace^tm1Unc; Ace2^tm1Pngr/Y
• Genetic Background: involves: 129P2/OlaHsd

cardiovascular system

hypotension ( J:77232 )

♂ • similar to Ace^tm1Unc homozygotes however normal cardiac contractility is seen at 6 months of age unlike in Ace^tm1Unc homozygotes

homeostasis/metabolism

decreased angiotensin II level ( J:100334 )

♂ • mice exposed to acid aspiration to induce acute lung injury show decreased angiotensin II levels in lung compared to single Ace2^tm1Pngr mutants

decreased circulating angiotensin II level ( J:100334 )

♂ • mice exposed to acid aspiration to induce acute lung injury show decreased angiotensin II levels in plasma compared to single Ace2^tm1Pngr mutants

decreased susceptibility to injury ( J:100334 )

♂ • mice show rescue of severe lung failure, edema formation, and histological lung changes induced by acid aspiration in single Ace2^tm1Pngr mutants

♂ • mice show rescue of the severe lung impairments seen in single Ace2^tm1Pngr mutants with endotoxin-induced acute lung injury

renal/urinary system

abnormal kidney morphology ( J:77232 )

♂ • similar to Ace^tm1Unc homozygotes