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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Alox5tm1Fun
targeted mutation 1, Colin D Funk
MGI:1857125
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Alox5tm1Fun/Alox5tm1Fun B6.129S2-Alox5tm1Fun MGI:3045876
hm2
Alox5tm1Fun/Alox5tm1Fun B6.129S2-Alox5tm1Fun/J MGI:3622808
hm3
Alox5tm1Fun/Alox5tm1Fun involves: 129S2/SvPas * C57BL/6 MGI:3045815


Genotype
MGI:3045876
hm1
Allelic
Composition
Alox5tm1Fun/Alox5tm1Fun
Genetic
Background
B6.129S2-Alox5tm1Fun
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alox5tm1Fun mutation (3 available); any Alox5 mutation (49 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
N
• homozygotes display no differences in infarct size following induction of focal cerebral ischemia (permanent or transient; 60 min) relative to wild-type




Genotype
MGI:3622808
hm2
Allelic
Composition
Alox5tm1Fun/Alox5tm1Fun
Genetic
Background
B6.129S2-Alox5tm1Fun/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alox5tm1Fun mutation (3 available); any Alox5 mutation (49 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
adipose tissue
• increased total fat pad weight

homeostasis/metabolism
• increased plasma VLDL/LDL cholesterol

skeleton
• increased bone mineral density

immune system
• upon adoptive transfer of IRBP1-20-specific T cells from wild-type B6 donors to wild-type and Alox5-deficient recipients, wild-type mice develop severe clinical uveitis, whereas mutant recipients show only mild and transient clinical disease




Genotype
MGI:3045815
hm3
Allelic
Composition
Alox5tm1Fun/Alox5tm1Fun
Genetic
Background
involves: 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alox5tm1Fun mutation (3 available); any Alox5 mutation (49 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
N
• homozygotes display a normal mean arterial pressure and heart rate relative to wild-type

hematopoietic system
N
• analysis of cell-surface markers CD3, CD4 and B220 on splenocytes from 4-wk- and 8-wk-old mice revealed no other abnormalities
• homozygotes show no changes in total blood cell populations or differential cell counts
• bone marrow analysis revealed no evidence of abnormal precursor cells of any lineage relative to wild-type
• homozygotes show no abnormalities up to 10 months of age under normal physiological conditions; however, their spleen is usually smaller relative to wild-type

immune system
N
• homozygotes display a normal reaction to endotoxin-induced shock
• also, homozygotes exhibit a normal reaction to ear inflammation induced by topical application of phorbol myristyl acetate
• neutrophil recruitment into the peritoneal cavity by a non-specific inflammatory challenge (glycogen) elicits a comparable (large) influx of polymorphonuclear leukocytes in both wild-type and mutant mice
• homozygotes show no abnormalities up to 10 months of age under normal physiological conditions; however, their spleen is usually smaller relative to wild-type
• conscious, unanaesthetized homozygotes show resistance to the lethal effects of platelet-activating factor (PAF): they display the same listless behavior as wild-type for the first 20 min but recover quickly
• mechanically ventilated, anesthetized homozygotes show no signs of broncho-constriction; however, broncho-constriction is observed after methacholine challenge
• homozygotes show a significantly reduced reaction to arachidonic acid-induced ear inflammation; administration of leukotrienes rescues this inflammatory phenotype: inflammation is rapid in onset and maximal swelling is reached between 30-60 min (J:21372)
• homozygotes display a significantly reduced chemotactic infiltration by polymorphonuclear leukocytes following induction of immune complex peritonitis (reverse passive Arthus reaction) (J:21372)
• in the thioglycollate-elicited peritonitis model, homozygotes only generate about 50% as much peritoneal leukocytosis as wild-type mice at 4.5 h after i.p. thioglycollate challenge; as expected, leukotriene B4 is absent from the mutant peritoneal fluid (J:75371)
• the specific arachidonate 5-lipoxygenase inhibitor, zileuton, has no effect on the peripheral white-cell count in mutant mice (J:75371)





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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory