Phenotypes associated with this allele

• Allele Symbol: Apoa1^tm1Unc
• Allele Name: targeted mutation 1, University of North Carolina
• Allele ID: MGI:1857127
• Summary: 9 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Apoa1^tm1Unc/Apoa1^tm1Unc	B6.129P2-Apoa1^tm1Unc	MGI:3783773
hm2	Apoa1^tm1Unc/Apoa1^tm1Unc	B6.129P2-Apoa1^tm1Unc/J	MGI:3580311
hm3	Apoa1^tm1Unc/Apoa1^tm1Unc	involves: 129P2/OlaHsd	MGI:3783784
hm4	Apoa1^tm1Unc/Apoa1^tm1Unc	involves: 129P2/OlaHsd * C57BL/6J	MGI:3783771
ht5	Apoa1^tm1Unc/Apoa1^+	B6.129P2-Apoa1^tm1Unc	MGI:3783774
ht6	Apoa1^tm1Unc/Apoa1^+	involves: 129P2/OlaHsd * C57BL/6J	MGI:3783772
cx7	Apoa1^tm1Unc/Apoa1^tm1Unc Ldlr^tm1Her/Ldlr^tm1Her	B6.129-Ldlr^tm1Her Apoa1^tm1Unc	MGI:3783838
cx8	Apoa1^tm1Unc/Apoa1^tm1Unc Tg(APOA1)1Rub/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3783803
cx9	Apoa1^tm1Unc/Apoa1^tm1Unc Tg(APOA2)3Rub/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3783804

Genotype
MGI:3783773

hm1

• Allelic Composition: Apoa1^tm1Unc/Apoa1^tm1Unc
• Genetic Background: B6.129P2-Apoa1^tm1Unc

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

decreased circulating cholesterol level ( J:22986 )

• plasma total cholesterol is 25% of wild-type

• plasma cholesteryl ester synthesis is reduced

Genotype
MGI:3580311

hm2

• Allelic Composition: Apoa1^tm1Unc/Apoa1^tm1Unc
• Genetic Background: B6.129P2-Apoa1^tm1Unc/J

homeostasis/metabolism

abnormal vascular wound healing ( J:118063 )

• reendothelialization is delayed compared to in wild-type mice following injury to the carotid artery

• mice exhibit a 52% larger area of non-reendothelialized carotid artery following injury compared to in wild-type mice

decreased circulating cholesterol level ( J:114205 )

• serum total cholesterol levels are reduced by one third compared to in wild-type mice

• esterified cholesterol is reduced 7-fold and free cholesterol 1.6-fold compared to in wild-type mice

decreased circulating HDL cholesterol level ( J:114205 )

• Apoa1-poor HDL and Apoa1-rich HDL

increased circulating cholesterol level ( J:114205 )

• serum total cholesterol, free cholesterol and esterified cholesterol levels in mice treated with silver nitrate to induce an acute phase response are increased more than in similarly treated wild-type mice

decreased circulating phospholipid level ( J:114205 )

• serum phospholipids levels are reduced by one third compared to in wild-type mice

increased circulating triglyceride level ( J:114205 )

• triglyceride levels in mice treated with silver nitrate to induce an acute phase response are increased moderately compared to in similarly treated wild-type mice

cardiovascular system

abnormal vascular wound healing ( J:118063 )

• reendothelialization is delayed compared to in wild-type mice following injury to the carotid artery

• mice exhibit a 52% larger area of non-reendothelialized carotid artery following injury compared to in wild-type mice

Genotype
MGI:3783784

hm3

• Allelic Composition: Apoa1^tm1Unc/Apoa1^tm1Unc
• Genetic Background: involves: 129P2/OlaHsd

homeostasis/metabolism

abnormal cellular cholesterol metabolism ( J:120501 )

• HDL cholesteryl ether clearance is over 20-fold higher than in wild-type mice and varies depending on the organ examined

• however, the clearance rate could be rescued by increasing plasma HDL cholesteryl ester levels

decreased cholesterol efflux ( J:129136 )

decreased circulating cholesterol level ( J:129136 )

decreased circulating HDL cholesterol level ( J:129136 )

decreased circulating phospholipid level ( J:129136 )

cellular

abnormal cellular cholesterol metabolism ( J:120501 )

• HDL cholesteryl ether clearance is over 20-fold higher than in wild-type mice and varies depending on the organ examined

• however, the clearance rate could be rescued by increasing plasma HDL cholesteryl ester levels

decreased cholesterol efflux ( J:129136 )

Genotype
MGI:3783771

hm4

• Allelic Composition: Apoa1^tm1Unc/Apoa1^tm1Unc
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

homeostasis/metabolism

decreased circulating cholesterol level ( J:2109 )

• at 8 weeks of age, total cholesterol levels are decreased to 33% of wild-type

decreased circulating HDL cholesterol level ( J:2109 )

• at 8 weeks of age, HDL levels are decreased to 17% of wild-type

Genotype
MGI:3783774

ht5

• Allelic Composition: Apoa1^tm1Unc/Apoa1⁺
• Genetic Background: B6.129P2-Apoa1^tm1Unc

homeostasis/metabolism

decreased circulating cholesterol level ( J:22986 )

• plasma total cholesterol is 67% of wild-type

Genotype
MGI:3783772

ht6

• Allelic Composition: Apoa1^tm1Unc/Apoa1⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

homeostasis/metabolism

decreased circulating cholesterol level ( J:2109 )

• at 8 weeks of age, total cholesterol levels are decreased to 54% of wild-type

decreased circulating HDL cholesterol level ( J:2109 )

• at 8 weeks of age, HDL levels are decreased to 40% of wild-type

Genotype
MGI:3783838

cx7

• Allelic Composition: Apoa1^tm1Unc/Apoa1^tm1Unc; Ldlr^tm1Her/Ldlr^tm1Her
• Genetic Background: B6.129-Ldlr^tm1Her Apoa1^tm1Unc

homeostasis/metabolism

increased circulating triglyceride level ( J:85174 )

• when fed regular chow or a high fat diet for 16 weeks, serum triglyceride levels are increased compared to similarly treated Ldlr^tm1Her homozygotes

decreased cholesterol level ( J:85174 )

• when fed a high fat diet, female mice exhibit a decrease in aortic cholesterol compared to similarly treated Ldlr^tm1Her homozygotes

• however, aortic cholesterol levels in male mice fed a high fat diet are equivalent to in similarly treated Ldlr^tm1Her homozygotes and female mice die before completion of the study

• when fed a high fat diet, mice exhibit a decrease in cholesterol content, specifically esterified cholesterol, in the adrenal gland compared to similarly treated Ldlr^tm1Her homozygotes

decreased circulating cholesterol level ( J:85174 )

• when mice are fed a high fat diet or regular chow, plasma cholesterol levels are decreased relative to similarly treated Ldlr^tm1Her homozygotes

• unlike male Ldlr^tm1Her homozygotes, plasma cholesterol levels fail to increased from weeks 8 to 16 on a high fat diet

• when fed a high fat diet, free cholesterol levels fail to increase as much as in similarly treated Ldlr^tm1Her homozygotes

• unlike Ldlr^tm1Her homozygotes, free cholesterol levels fail to increased from weeks 8 to 16 on a high fat diet

• when mice are fed a high fat diet or regular chow, esterified cholesterol levels are decreased relative to similarly treated Ldlr^tm1Her homozygotes

• unlike male Ldlr^tm1Her homozygotes, esterified cholesterol levels fail to increased from weeks 8 to 16 on a high fat diet

• when fed a high fat diet, mice exhibit a lesser increase in VLDL and LDL (3-fold) compared to Ldlr^tm1Her homozygotes (6-fold)

decreased circulating HDL cholesterol level ( J:85174 )

• when fed regular chow or a high fat diet for 16 weeks, serum HDL levels are decreased compared to similarly treated Ldlr^tm1Her homozygotes

decreased circulating LDL cholesterol level ( J:85174 )

• when fed a high fat diet, mice exhibit a lesser increase in VLDL and LDL (3-fold) compared to Ldlr^tm1Her homozygotes (6-fold)

decreased circulating VLDL cholesterol level ( J:85174 )

• when fed a high fat diet, mice exhibit a lesser increase in VLDL and LDL (3-fold) compared to Ldlr^tm1Her homozygotes (6-fold)

decreased liver cholesterol level ( J:85174 )

• when fed a high fat diet, mice exhibit a reduced increase of 2.5-fold in liver cholesterol content compared to 11-fold in similarly treated Ldlr^tm1Her homozygotes

xanthoma ( J:85174 )

• female mice develop skin lesions when fed a high fat diet

• total skin cholesterol content is increased 12- to 13-fold and 5.5-fold for free cholesterol compared to in Ldlr^tm1Her homozygotes

liver/biliary system

abnormal liver morphology ( J:85174 )

• when fed a high fat diet, mice exhibit smaller diameter lipid droplets than in Ldlr^tm1Her homozygotes

• when fed a high fat diet, mice exhibit an accumulation of inflammatory cells such as neutrophils and leukocytes around the hepatic vein unlike in Ldlr^tm1Her homozygotes

decreased liver cholesterol level ( J:85174 )

• when fed a high fat diet, mice exhibit a reduced increase of 2.5-fold in liver cholesterol content compared to 11-fold in similarly treated Ldlr^tm1Her homozygotes

endocrine/exocrine glands

abnormal adrenal gland morphology ( J:85174 )

• when fed a high fat diet, mice exhibit an decrease in cholesterol content, specifically esterified cholesterol, in the adrenal gland compared to in similarly treated Ldlr^tm1Her homozygotes

abnormal adrenal gland zona fasciculata morphology ( J:85174 )

• when fed a high fat diet, mice exhibit a severe depletion of cytoplasmic lipid droplets unlike in Ldlr^tm1Her homozygotes

integument

thick dermal layer ( J:85174 )

• when fed a high fat diet, mice develop a thickened dermal layer with macrophage infiltrate and cholesterol deposits

• dermal thickening associated with a high fat diet is more severe in female mice than male mice

skin lesions ( J:85174 )

• when fed a high fat diet, female mice develop skin lesions beginning at 9 weeks with scratching and lesion areas that progress until animals cease eating and drinking

• skin lesions observed in mice fed a high fat diet are different than the non-fatal skin thickening observed in similarly treated Ldlr^tm1Her homozygotes

spontaneous skin ulceration ( J:85174 )

• when fed a high fat diet, female mice exhibit severe ulcerations on thickened skin of the abdomen, neck and front limbs

increased pruritus ( J:85174 )

• when fed a high fat diet, female mice develop pruritus (itching) and must be euthanized before the end of the 16 week study period

Genotype
MGI:3783803

cx8

• Allelic Composition: Apoa1^tm1Unc/Apoa1^tm1Unc; Tg(APOA1)1Rub/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

homeostasis/metabolism

increased cholesterol efflux ( J:129136 )

• compared to in Apoa1^tm1Unc homozygotes with or without Tg(APOA2)3Rub

increased circulating cholesterol level ( J:129136 )

• compared to in Apoa1^tm1Unc homozygotes with or without Tg(APOA2)3Rub

increased circulating HDL cholesterol level ( J:129136 )

• compared to in Apoa1^tm1Unc homozygotes with or without Tg(APOA2)3Rub

increased circulating triglyceride level ( J:129136 )

• compared to in Apoa1^tm1Unc homozygotes with or without Tg(APOA2)3Rub

cellular

increased cholesterol efflux ( J:129136 )

• compared to in Apoa1^tm1Unc homozygotes with or without Tg(APOA2)3Rub

Genotype
MGI:3783804

cx9

• Allelic Composition: Apoa1^tm1Unc/Apoa1^tm1Unc; Tg(APOA2)3Rub/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

homeostasis/metabolism

decreased circulating cholesterol level ( J:129136 )

• while serum cholesterol is decreased there is an increase in the ratio of unesterified to esterified cholesterol compared to in Apoa1^tm1Unc homozygotes

increased circulating triglyceride level ( J:129136 )

• triglyceride levels are increased compared to in Apoa1^tm1Unc homozygotes