Phenotypes associated with this allele

• Allele Symbol: Cd247^tm1Lov
• Allele Name: targeted mutation 1, Paul E Love
• Allele ID: MGI:1857142
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cd247^tm1Lov/Cd247^tm1Lov	B6.129S4-Cd247^tm1Lov/J	MGI:5009812
hm2	Cd247^tm1Lov/Cd247^tm1Lov	involves: 129S4/SvJae	MGI:2448649
ht3	Cd247^tm1Lov/Cd247^tm2.1Lov	involves: 129S4/SvJae	MGI:5468138
cx4	Cd247^tm1Lov/Cd247^tm1Lov Fcer1g^tm1Rav/Fcer1g^tm1Rav	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6	MGI:2448651

Genotype
MGI:5009812

hm1

• Allelic Composition: Cd247^tm1Lov/Cd247^tm1Lov
• Genetic Background: B6.129S4-Cd247^tm1Lov/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

enlarged lateral ventricles ( J:66246 )

• 22% of P13 mice and 20% of adult mice have enlarged lateral ventricles

abnormal lateral geniculate nucleus morphology ( J:66246 )

• area of the ipsilateral projection is increased compared to controls

enhanced long-term potentiation ( J:66246 )

• LTP in response to tetanus is enhanced by approximately 248% as compared to wild type

abnormal long-term depression ( J:66246 )

• 900 pulses at 1 Hz enhances baseline LTD, but LTD is unchanged in wild-type

reduced long-term depression ( J:66246 )

• 900 pulses at 0.5 Hz does not induce significant LTD in contrast to wild-type response

Genotype
MGI:2448649

hm2

• Allelic Composition: Cd247^tm1Lov/Cd247^tm1Lov
• Genetic Background: involves: 129S4/SvJae

immune system

decreased thymocyte number ( J:14170 )

• average number of thymocytes about 14% of control numbers

decreased double-positive T cell number ( J:14170 )

• decreased number of double positive thymocytes

decreased CD4-positive, alpha-beta T cell number ( J:14170 )

• about 1% normal levels in thymus

absent gamma-delta intraepithelial T cell ( J:46914 )

• TCR gamma-delta T cells are virtually absent while alpha-beta lineages are readily detectable among intestinal epithelial lymphocytes

abnormal T-helper 1 cell morphology ( J:14170 )

• Th1 memory cell phenotype

abnormal single-positive T cell number ( J:14170 )

• reduced in number but significant numbers of single positive T-cells expressing low levels of CD5 and barely detectable levels of CD3 epsilon and TCR beta are found in lymph nodes present never the less

• deficient in both single positive thymocyte types

abnormal T-helper 1 physiology ( J:46914 )

• can be stimulated to produce large quantities of interferon gamma

• can be induced to produce Il2

nervous system

abnormal brain morphology ( J:66246 )

abnormal superior colliculus morphology ( J:66246 )

• retinal projections to the ipsilateral superior colliculus occasionally found

abnormal lateral geniculate nucleus morphology ( J:66246 )

• the area of ipsilateral projections to the dorsal lateral geniculate nucleus is enlarged

• ectopic projections are sometimes also found in mediodorsal areas

abnormal CNS synaptic transmission ( J:66246 )

abnormal excitatory postsynaptic potential ( J:66246 )

• increased slope of the field excitatory post synaptic potential

enhanced long-term potentiation ( J:66246 )

• in CA1 of the hippocampus after titanic stimulation

absent long-term depression ( J:66246 )

• not detectable

hematopoietic system

decreased thymocyte number ( J:14170 )

• average number of thymocytes about 14% of control numbers

decreased double-positive T cell number ( J:14170 )

• decreased number of double positive thymocytes

decreased CD4-positive, alpha-beta T cell number ( J:14170 )

• about 1% normal levels in thymus

absent gamma-delta intraepithelial T cell ( J:46914 )

• TCR gamma-delta T cells are virtually absent while alpha-beta lineages are readily detectable among intestinal epithelial lymphocytes

abnormal T-helper 1 cell morphology ( J:14170 )

• Th1 memory cell phenotype

abnormal single-positive T cell number ( J:14170 )

• reduced in number but significant numbers of single positive T-cells expressing low levels of CD5 and barely detectable levels of CD3 epsilon and TCR beta are found in lymph nodes present never the less

• deficient in both single positive thymocyte types

abnormal T-helper 1 physiology ( J:46914 )

• can be stimulated to produce large quantities of interferon gamma

• can be induced to produce Il2

endocrine/exocrine glands

decreased thymocyte number ( J:14170 )

• average number of thymocytes about 14% of control numbers

Genotype
MGI:5468138

ht3

• Allelic Composition: Cd247^tm1Lov/Cd247^tm2.1Lov
• Genetic Background: involves: 129S4/SvJae

immune system

immune system phenotype ( J:192915 )

N • mice do not develop spontaneous autoimmune disease

Genotype
MGI:2448651

cx4

• Allelic Composition: Cd247^tm1Lov/Cd247^tm1Lov; Fcer1g^tm1Rav/Fcer1g^tm1Rav
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6

immune system

decreased thymocyte number ( J:46914 )

• average number of thymocytes between 10 and 30% of control numbers

• almost entirely double positive or double negative

• express very low but detectable levels of TCR

absent gamma-delta intraepithelial T cell ( J:46914 )

• TCR gamma-delta T cells are virtually absent while alpha-beta lineages are readily detectable among intestinal epithelial lymphocytes

abnormal effector T cell morphology ( J:46914 )

• detectable in lymph nodes and the spleen

• a high percentage with activated or memory phenotypes

impaired natural killer cell mediated cytotoxicity ( J:64764 )

• natural cytoxicity towards YAC-1, Bw15.02, C4.4.25, RMA, and RMA/S cells is defective compared to in wild-type cells

abnormal T-helper 1 physiology ( J:46914 )

• can be stimulated to produce large quantities of interferon gamma

• can be induced to produce Il2

abnormal T cell activation ( J:46914 )

• refractory to direct TCR stimulation in vitro

hematopoietic system

decreased thymocyte number ( J:46914 )

• average number of thymocytes between 10 and 30% of control numbers

• almost entirely double positive or double negative

• express very low but detectable levels of TCR

absent gamma-delta intraepithelial T cell ( J:46914 )

• TCR gamma-delta T cells are virtually absent while alpha-beta lineages are readily detectable among intestinal epithelial lymphocytes

abnormal effector T cell morphology ( J:46914 )

• detectable in lymph nodes and the spleen

• a high percentage with activated or memory phenotypes

impaired natural killer cell mediated cytotoxicity ( J:64764 )

• natural cytoxicity towards YAC-1, Bw15.02, C4.4.25, RMA, and RMA/S cells is defective compared to in wild-type cells

abnormal T-helper 1 physiology ( J:46914 )

• can be stimulated to produce large quantities of interferon gamma

• can be induced to produce Il2

abnormal T cell activation ( J:46914 )

• refractory to direct TCR stimulation in vitro

endocrine/exocrine glands

decreased thymocyte number ( J:46914 )

• average number of thymocytes between 10 and 30% of control numbers

• almost entirely double positive or double negative

• express very low but detectable levels of TCR