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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Cd8atm1Mak
targeted mutation 1, Tak Mak
MGI:1857149
Summary 13 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Cd8atm1Mak/Cd8atm1Mak B6.129S2-Cd8atm1Mak MGI:3789192
hm2
 		Cd8atm1Mak/Cd8atm1Mak involves: 129S2/SvPas MGI:3800777
hm3
 		Cd8atm1Mak/Cd8atm1Mak involves: 129S2/SvPas * C57BL/6 * DBA/2 MGI:2662259
hm4
 		Cd8atm1Mak/Cd8atm1Mak PL.129S2(B6)-Cd8atm1Mak/Rdgz MGI:3052051
hm5
 		Cd8atm1Mak/Cd8atm1Mak SJL.129S2(B6)-Cd8atm1Mak/Rdgz MGI:3052052
ht6
 		Cd8atm1Asin/Cd8atm1Mak involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * FVB/N MGI:3760623
cx7
 		Cd8atm1Mak/Cd8atm1Mak
X/Yaa 		BXSB.129S2(B6)-Cd8atm1Mak/4Dcr MGI:6104236
cx8
 		Cd8atm1Mak/Cd8atm1Mak
Il15tm1Imx/Il15tm1Imx
X/Yaa 		BXSB.Cg-Cd8atm1Mak Il15tm1Imx/Dcr MGI:6105946
cx9
 		Cd4tm1Mak/Cd4tm1Mak
Cd8atm1Mak/Cd8atm1Mak 		involves: 129S2/SvPas MGI:3800776
cx10
 		Cd8atm1Mak/Cd8atm1Mak
Irf2tm1Mak/Irf2tm1Mak 		involves: 129S2/SvPas * C57BL/6 MGI:3630273
cx11
 		Cd8atm1Mak/Cd8atm1Mak
Tgfb1tm1Doe/Tgfb1tm1Doe 		involves: 129S2/SvPas * C57BL/6 * CF-1 MGI:3622472
cx12
 		Cd4tm1Mak/Cd4tm1Mak
Cd8atm1Mak/Cd8atm1Mak 		involves: 129S2/SvPas * C57BL/6 * DBA/2 MGI:3800764
cx13
 		Cd8atm1Mak/Cd8atm1Mak
Zbtb7bhd/Zbtb7bhd 		involves: B6.129S2-Cd8atm1Mak * B6.Cg-Thpokhd MGI:3573951


Genotype
MGI:3789192
hm1
Allelic
Composition		 Cd8atm1Mak/Cd8atm1Mak
Genetic
Background		 B6.129S2-Cd8atm1Mak
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Mak mutation (7 available); any Cd8a mutation (39 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal response to transplant ( J:91742 )
• skin grafts from OVA expressing transgenic mice ( Tg(CAG-OVA)916Jen) shrink rapidly to 10-30% of their original size on mutant hosts but are then retained indefinitely in healthy condition




Genotype
MGI:3800777
hm2
Allelic
Composition		 Cd8atm1Mak/Cd8atm1Mak
Genetic
Background		 involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Mak mutation (7 available); any Cd8a mutation (39 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:110749 )
N
• mice clear polyomavirus within one month of infection similar to controls




Genotype
MGI:2662259
hm3
Allelic
Composition		 Cd8atm1Mak/Cd8atm1Mak
Genetic
Background		 involves: 129S2/SvPas * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Mak mutation (7 available); any Cd8a mutation (39 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal cytotoxic T cell physiology ( J:68956 )
• lacks cytotoxic T cell response
• no response to class I MHC alloantigen but normal response to class II

immune system
abnormal cytotoxic T cell physiology ( J:68956 )
• lacks cytotoxic T cell response
• no response to class I MHC alloantigen but normal response to class II
increased susceptibility to Picornaviridae infection ( J:92227 )
• inflammation is mostly resolved by 45 days after TMEV infection on the resistant C57BL/6 background
• infectious virus remains at 45 days, however, disease is absent by 10 months on the resistant C57BL/6 background

nervous system
demyelination ( J:92227 )
• on the resistant C57BL/6 background, foci of demyelination at 45 days after TMEV infection




Genotype
MGI:3052051
hm4
Allelic
Composition		 Cd8atm1Mak/Cd8atm1Mak
Genetic
Background		 PL.129S2(B6)-Cd8atm1Mak/Rdgz
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Mak mutation (7 available); any Cd8a mutation (39 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased susceptibility to Picornaviridae infection ( J:92227 )
• inflammation persists in the susceptible PL/J background

nervous system
demyelination ( J:92227 )
• demyelination was severe on both the susceptible PL/J and SJL/J backgrounds after infection with TMEV




Genotype
MGI:3052052
hm5
Allelic
Composition		 Cd8atm1Mak/Cd8atm1Mak
Genetic
Background		 SJL.129S2(B6)-Cd8atm1Mak/Rdgz
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Mak mutation (7 available); any Cd8a mutation (39 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased susceptibility to Picornaviridae infection ( J:92227 )
• inflammation persists in the susceptible SJL/J background

nervous system
demyelination ( J:92227 )
• demyelination was severe on both the susceptible PL/J and SJL/J backgrounds after infection with TMEV




Genotype
MGI:3760623
ht6
Allelic
Composition		 Cd8atm1Asin/Cd8atm1Mak
Genetic
Background		 involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Asin mutation (0 available); any Cd8a mutation (39 available)
Cd8atm1Mak mutation (7 available); any Cd8a mutation (39 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal positive T cell selection ( J:126026 )
• the conversion of double positive T cells into single-positive CD8 T cells is 2.5 fold greater than in wild-type littermates
abnormal T cell subpopulation ratio ( J:126026 )
• the ratio of CD4/CD8 T cells in the thymus, spleen, and lymph nodes is reversed from 1.5 to 0.5
increased CD8-positive, alpha-beta T cell number ( J:126026 )
• absolute numbers of mature CD8-positive T cell are about 2 fold higher in the spleen, lymph nodes, and thymus

hematopoietic system
abnormal positive T cell selection ( J:126026 )
• the conversion of double positive T cells into single-positive CD8 T cells is 2.5 fold greater than in wild-type littermates
abnormal T cell subpopulation ratio ( J:126026 )
• the ratio of CD4/CD8 T cells in the thymus, spleen, and lymph nodes is reversed from 1.5 to 0.5
increased CD8-positive, alpha-beta T cell number ( J:126026 )
• absolute numbers of mature CD8-positive T cell are about 2 fold higher in the spleen, lymph nodes, and thymus




Genotype
MGI:6104236
cx7
Allelic
Composition		 Cd8atm1Mak/Cd8atm1Mak
X/Yaa
Genetic
Background		 BXSB.129S2(B6)-Cd8atm1Mak/4Dcr
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Mak mutation (7 available); any Cd8a mutation (39 available)
Yaa mutation (24 available); any Yaa mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased susceptibility to systemic lupus erythematosus ( J:179430 )
• compared with BXSB/MpJ males that carry Yaa, the additional ablation of CD8A accelerates the mortality from the spontaneous lupus erythematosus-like syndrome such that mortality occurs as early as 12 to 15 weeks of age and mean survival is only 20 weeks of age

mortality/aging




Genotype
MGI:6105946
cx8
Allelic
Composition		 Cd8atm1Mak/Cd8atm1Mak
Il15tm1Imx/Il15tm1Imx
X/Yaa
Genetic
Background		 BXSB.Cg-Cd8atm1Mak Il15tm1Imx/Dcr
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Mak mutation (7 available); any Cd8a mutation (39 available)
Il15tm1Imx mutation (5 available); any Il15 mutation (44 available)
Yaa mutation (24 available); any Yaa mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging

renal/urinary system
renal glomerular immunoglobulin deposits ( J:179430 )
• glomerular deposition of IgG at 14 weeks of age is more severe than that of BXSB/MpJ males

immune system
increased monocyte cell number ( J:179430 )
• at 14 weeks of age relative to BSXB/MpJ or BXSB.B6=Yaa+ males
abnormal spleen B cell follicle morphology ( J:179430 )
• scattered follicular distribution in the spleen and large extrafollicular accumulations of CD138+ plasma cells and plasmablasts are found at 14 weeks of age
increased splenocyte number ( J:179430 )
• by 14 weeks of age compared with BXSB/MpJ males and the splenocytes have an increased proportion of monocytes, increased ICOS expression on CD4+ T cells, and increased FAS expression on B cells
increased IgG1 level ( J:179430 )
• significantly higher than BXSB/MpJ males as early as 4 weeks of age, the earliest timepoint assessed
increased IgG2a level ( J:179430 )
• elevated earlier and significantly higher than BXSB/MpJ males as early as 4 weeks of age, the earliest timepoint assessed
increased IgG2b level ( J:179430 )
• elevated earlier and significantly higher than BXSB/MpJ males as early as 4 weeks of age, the earliest timepoint assessed
increased susceptibility to systemic lupus erythematosus ( J:179430 )
• while BXSB/MpJ males develop SLE-like disease and have a mean survival of 32 weeks, males homozygous for both null alleles die earlier than those with just one null allele and with a survival curve essentially the same as that of beta 2 microglobulin null Yaa carrying males, with a mean survival of only 18 weeks
increased anti-nuclear antigen antibody level ( J:179430 )
• elevated over BXSB/MpJ males at 6 and 14 weeks of age

hematopoietic system
increased monocyte cell number ( J:179430 )
• at 14 weeks of age relative to BSXB/MpJ or BXSB.B6=Yaa+ males
abnormal spleen B cell follicle morphology ( J:179430 )
• scattered follicular distribution in the spleen and large extrafollicular accumulations of CD138+ plasma cells and plasmablasts are found at 14 weeks of age
increased splenocyte number ( J:179430 )
• by 14 weeks of age compared with BXSB/MpJ males and the splenocytes have an increased proportion of monocytes, increased ICOS expression on CD4+ T cells, and increased FAS expression on B cells
increased IgG1 level ( J:179430 )
• significantly higher than BXSB/MpJ males as early as 4 weeks of age, the earliest timepoint assessed
increased IgG2a level ( J:179430 )
• elevated earlier and significantly higher than BXSB/MpJ males as early as 4 weeks of age, the earliest timepoint assessed
increased IgG2b level ( J:179430 )
• elevated earlier and significantly higher than BXSB/MpJ males as early as 4 weeks of age, the earliest timepoint assessed




Genotype
MGI:3800776
cx9
Allelic
Composition		 Cd4tm1Mak/Cd4tm1Mak
Cd8atm1Mak/Cd8atm1Mak
Genetic
Background		 involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd4tm1Mak mutation (5 available); any Cd4 mutation (85 available)
Cd8atm1Mak mutation (7 available); any Cd8a mutation (39 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased susceptibility to viral infection ( J:110749 )
• virus is still detectable in bone marrow, heart, and other organs 1 month after polyomavirus infection compared to controls that clear the infection by one month




Genotype
MGI:3630273
cx10
Allelic
Composition		 Cd8atm1Mak/Cd8atm1Mak
Irf2tm1Mak/Irf2tm1Mak
Genetic
Background		 involves: 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Mak mutation (7 available); any Cd8a mutation (39 available)
Irf2tm1Mak mutation (1 available); any Irf2 mutation (24 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
integument
abnormal skin condition ( J:66034 )
• no skin symptoms are observed up to 4 months of age compared to Irf2-null mice




Genotype
MGI:3622472
cx11
Allelic
Composition		 Cd8atm1Mak/Cd8atm1Mak
Tgfb1tm1Doe/Tgfb1tm1Doe
Genetic
Background		 involves: 129S2/SvPas * C57BL/6 * CF-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Mak mutation (7 available); any Cd8a mutation (39 available)
Tgfb1tm1Doe mutation (4 available); any Tgfb1 mutation (35 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:99033 )
• majority of double mutants die by 35 days of age




Genotype
MGI:3800764
cx12
Allelic
Composition		 Cd4tm1Mak/Cd4tm1Mak
Cd8atm1Mak/Cd8atm1Mak
Genetic
Background		 involves: 129S2/SvPas * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd4tm1Mak mutation (5 available); any Cd4 mutation (85 available)
Cd8atm1Mak mutation (7 available); any Cd8a mutation (39 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
small thymus medulla ( J:12572 )
• the medulla is reduced in size
abnormal T cell differentiation ( J:12572 )
• there is a 5-fold drop in the number of mature alphabeta TCR+ HAS- T cells in the thymus
• alphabeta TCR expression on these mature double negative cells is lower than in mature T cells of wild-type mice
• despite lack of CD4 and CD8 expression, alphabeta T cells are still present in the periphery
abnormal double-negative T cell morphology ( J:12572 )
• "mature" double negative thymocytes are found that are alphabeta TCR+ HAS-
decreased double-positive T cell number ( J:12572 )
• double positive T cells are absent
abnormal T cell number ( J:12572 )
• the alphabeta T cell number in lymph nodes varies from 5% to 50% of normal
abnormal gamma-delta T cell number ( J:12572 )
• the absolute number of gammadelta T cells is decreased though the relative percentage of lymph node is increased from 1% to 3%
decreased cytotoxic T cell cytolysis ( J:12572 )
• LCMV-specific class I MHC-restricted cytotoxic T cell responses are not activated in these mice
• alloantigen specific lysis of target cells by T cells in mixed lymphocyte culture is reduced
• the lysis of cells is specific for class I MHC antigens
increased length of allograft survival ( J:12572 )
• tail skin graphs from donor mice of the same MHC haplotypes are not rejected within a three month period compared to controls that have no graph survival after 18 days
• when donor mice have a different MHC haplotype, skin graft rejection is similar to controls

hematopoietic system
small thymus medulla ( J:12572 )
• the medulla is reduced in size
abnormal T cell differentiation ( J:12572 )
• there is a 5-fold drop in the number of mature alphabeta TCR+ HAS- T cells in the thymus
• alphabeta TCR expression on these mature double negative cells is lower than in mature T cells of wild-type mice
• despite lack of CD4 and CD8 expression, alphabeta T cells are still present in the periphery
abnormal double-negative T cell morphology ( J:12572 )
• "mature" double negative thymocytes are found that are alphabeta TCR+ HAS-
abnormal T cell number ( J:12572 )
• the alphabeta T cell number in lymph nodes varies from 5% to 50% of normal
decreased double-positive T cell number ( J:12572 )
• double positive T cells are absent
abnormal gamma-delta T cell number ( J:12572 )
• the absolute number of gammadelta T cells is decreased though the relative percentage of lymph node is increased from 1% to 3%
decreased cytotoxic T cell cytolysis ( J:12572 )
• LCMV-specific class I MHC-restricted cytotoxic T cell responses are not activated in these mice
• alloantigen specific lysis of target cells by T cells in mixed lymphocyte culture is reduced
• the lysis of cells is specific for class I MHC antigens

endocrine/exocrine glands
small thymus medulla ( J:12572 )
• the medulla is reduced in size




Genotype
MGI:3573951
cx13
Allelic
Composition		 Cd8atm1Mak/Cd8atm1Mak
Zbtb7bhd/Zbtb7bhd
Genetic
Background		 involves: B6.129S2-Cd8atm1Mak * B6.Cg-Thpokhd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Mak mutation (7 available); any Cd8a mutation (39 available)
Zbtb7bhd mutation (0 available); any Zbtb7b mutation (26 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
absent CD4-positive, alpha-beta T cells ( J:95867 )
• lack mature, single-positive CD4+ thymocytes or peripheral CD4+ T cells

immune system
absent CD4-positive, alpha-beta T cells ( J:95867 )
• lack mature, single-positive CD4+ thymocytes or peripheral CD4+ T cells




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory