Analysis Tools|
Allele Symbol Allele Name Allele ID |
Gzmbtm1Ley targeted mutation 1, Timothy J Ley MGI:1857156 |
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| Summary |
11 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• impaired ability of NK cells to induce DNA fragmenting but normal ability to induce membrane damage
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• impaired ability of NK cells to induce DNA fragmenting but normal ability to induce membrane damage
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• specific activity of caspase 3 executioner protease in cytoplasm of CTLs is decreased
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• cytotoxic T lymphocytes (CTLs) defective in ability to induce rapid DNA fragmentation and apoptosis in allogeneic target cells
• with long incubation, fragmentation is partially and cell lysis completely rescued
• normal activation and proliferation of cytotoxic T lymphocytes (CTLs) in mixed lymphocyte cultures (MLCs)
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• cytotoxic T lymphocytes (CTLs) defective in ability to induce rapid DNA fragmentation and apoptosis in allogeneic target cells
• with long incubation, fragmentation is partially and cell lysis completely rescued
• normal activation and proliferation of cytotoxic T lymphocytes (CTLs) in mixed lymphocyte cultures (MLCs)
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice challenged with RMAS or B16 tumors exhibit decreased lethality compared with similarly treated wild-type mice
• mice challenged with MB0 cells exhibit decreased lethality compared with similarly treated wild-type mice
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• NK and CD8+ T cell death in the tumor ascites from mice transplanted with RMAS cells is decreased compared to that of similarly treated wild-type mice and further decreased after T reg depletion
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• NK and CD8+ T cell death in the tumor ascites from mice transplanted with RMAS cells is decreased compared to that of similarly treated wild-type mice and further decreased after T reg depletion
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• mice transplanted with labeled RMAS tumor cells exhibit reduced tumor burden compared with similarly treated wild-type mice
• adoptive transfer with wild-type T regulatory cells exhibit tumor growth that is 40% of that in similarly treated wild-type mice
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• NK and CD8+ T cell death in the tumor ascites from mice transplanted with RMAS cells is decreased compared to that of similarly treated wild-type mice and further decreased after T reg depletion
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• NK and CD8+ T cell death in the tumor ascites from mice transplanted with RMAS cells is decreased compared to that of similarly treated wild-type mice and further decreased after T reg depletion
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• NK and CD8+ T cell death in the tumor ascites from mice transplanted with RMAS cells is decreased compared to that of similarly treated wild-type mice and further decreased after T reg depletion
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• WNV-infected double homozygotes start to die at day 10 post-infection (p.i.); wild-type mice die between 10-13 days p.i.
• 70% of WNV-infected double homozygotes versus 29% of wild-type succumb to encephalitis by day 16 p.i.
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| N |
• double homozygotes develop a normal contact hypersensitivity (CHS) response (based on increase in ear thickness) to trinitrophenyl (TNP) relative to wild-type mice
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• decreased clearance and survival after infection with Trypanosoma cruzi
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• double homozygotes display increased susceptibility to West Nile flavivirus (WNV) infection relative to wild-type
• double mutants show a higher level of infectious virus in brain relative to wild-type or Prf1tm1Sdz mice at the same time point
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• WNV-infected triple homozygotes display prolonged mean survival time (MST) but significantly increased mortality (50%) relative to either wild-type (29%) or Prf1tm1Sdz (18%) mice
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| N |
• triple homozygotes develop a normal contact hypersensitivity (CHS) response (based on increase in ear thickness) to trinitrophenyl (TNP) relative to wild-type mice
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• decreased clearance and survival after infection with Trypanosoma cruzi
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• triple homozygotes display increased susceptibility to West Nile flavivirus (WNV) infection relative to wild-type
• triple mutants show a higher level of infectious virus in brain relative to wild-type or Prf1tm1Sdz mice at the same time point
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• decreased clearance and survival after infection with Trypanosoma cruzi
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• as in Serpinb9tm1.1Pib homozygotes in mice immunized with apoptotic cells displaying ovalbumin
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• as in Serpinb9tm1.1Pib homozygotes in mice immunized with apoptotic cells displaying ovalbumin
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• as in Serpinb9tm1.1Pib homozygotes in mice immunized with apoptotic cells displaying ovalbumin
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• after LCMV infection, numbers of LCMV-specific CTLs are same as wild-type
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• level of apoptosis of LCMV-specific CTLs in mutants is same as in wild-type compared to increase seen in single knockouts
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• after LCMV infection, numbers of LCMV-specific CTLs are same as wild-type
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• level of apoptosis of LCMV-specific CTLs in mutants is same as in wild-type compared to increase seen in single knockouts
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• in vitro- and ex vivo-derived cytotoxic T cells and NK cells from double mutant mice induce lysis in various target cells, at levels and with kinetics similar to those of single mutant or C57BL/6 mice
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• in vitro- and ex vivo-derived cytotoxic T cells and NK cells from double mutant mice fail to induce apoptotic nuclear damage in target cells
• in double mutants, Tc/NK-mediated target cell DNA fragmentation is undetectable, even after extended incubation periods (10 h)
• in contrast, Tc/NK-mediated target cell DNA fragmentation is normal in Gzmatm1Simn and only impaired in Gzmbtm1Ley mice in short-term (2-4 h), but not long-term (4-10 h), nucleolytic assays
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• in vitro- and ex vivo-derived cytotoxic T cells and NK cells from double mutant mice fail to induce apoptotic nuclear damage in target cells
• in double mutants, Tc/NK-mediated target cell DNA fragmentation is undetectable, even after extended incubation periods (10 h)
• in contrast, Tc/NK-mediated target cell DNA fragmentation is normal in Gzmatm1Simn and only impaired in Gzmbtm1Ley mice in short-term (2-4 h), but not long-term (4-10 h), nucleolytic assays
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice challenged with RMAS or B16 tumors exhibit increased lethality compared with similarly treated wild-type mice
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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