Phenotypes associated with this allele

• Allele Symbol: Drd3^tm1Dac
• Allele Name: targeted mutation 1, Domenico Accili
• Allele ID: MGI:1857159
• Summary: 9 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Drd3^tm1Dac/Drd3^tm1Dac	B6.129S4-Drd3^tm1Dac	MGI:4839990
hm2	Drd3^tm1Dac/Drd3^tm1Dac	B6.129S4-Drd3^tm1Dac/J	MGI:4839954
hm3	Drd3^tm1Dac/Drd3^tm1Dac	involves: 129S4/SvJae	MGI:4839961
hm4	Drd3^tm1Dac/Drd3^tm1Dac	involves: 129S4/SvJae * C57BL/6	MGI:3614420
hm5	Drd3^tm1Dac/Drd3^tm1Dac	involves: 129S4/SvJae * C57BL/6J	MGI:4839942
ht6	Drd3^tm1Dac/Drd3^+	involves: 129S4/SvJae * C57BL/6	MGI:3614421
ht7	Drd3^tm1Dac/Drd3^+	involves: 129S4/SvJae * C57BL/6J	MGI:4839943
cx8	Drd2^tm1Ebo/Drd2^tm1Ebo Drd3^tm1Dac/Drd3^tm1Dac	involves: 129S2/SvPas * 129S4/SvJae * C57BL/6	MGI:4839981
cx9	Drd1^tm1Jcd/Drd1^tm1Jcd Drd3^tm1Dac/Drd3^tm1Dac	involves: 129S4/SvJae * C57BL/6	MGI:4839955

Genotype
MGI:4839990

hm1

• Allelic Composition: Drd3^tm1Dac/Drd3^tm1Dac
• Genetic Background: B6.129S4-Drd3^tm1Dac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

decreased startle reflex ( J:154428 )

• mice exhibit reduced acoustic startle response amplitude compared with wild-type mice

Genotype
MGI:4839954

hm2

• Allelic Composition: Drd3^tm1Dac/Drd3^tm1Dac
• Genetic Background: B6.129S4-Drd3^tm1Dac/J

behavior/neurological

decreased anxiety-related response ( J:102600 )

• mice exhibit increased entry into open arms in an elevated plus maze compared with wild-type mice

abnormal reflex ( J:96803 )

• mice exhibit more large-reflex amplitudes for both monosynaptic 'stretch' reflex (MSR) and longer-latency reflex responses compared with wild-type mice

• mice treated with 1 or 5 um dopamine exhibit led to an increase in MSR amplitude and reflex strength unlike in similarly treated wild-type mice

• mice treated with a D3 receptor antagonist do not exhibit an increase in monosynaptic reflex strength unlike in similarly treated wild-type mice

• mice treated with D2 receptor agonists fail to exhibit a decrease in monosynaptic reflex strength compared with similarly treated wild-type mice

• mice treated with pergolide do not exhibit a difference in monosynaptic and longer-latency reflex amplitudes unlike in similarly treated wild-type mice

• mice treated with GR 103691 exhibit shorter monosynaptic than longer-latency reflex amplitude unlike in wild-type mice

abnormal motor coordination/balance ( J:102600 )

• on day 2, mice exhibit improved performance on a rotarod compared with wild-type mice

increased vertical activity ( J:102600 )

increased locomotor activity ( J:102600 )

• in an open field compared with wild-type mice

homeostasis/metabolism

abnormal physiological response to xenobiotic ( J:96803 )

• mice treated with a D3 receptor antagonist do not exhibit an increase in monosynaptic reflex strength unlike in similarly treated wild-type mice

• mice treated with D2 receptor agonists fail to exhibit a decrease in monosynaptic reflex strength compared with similarly treated wild-type mice

• mice treated with pergolide do not exhibit a difference in monosynaptic and longer-latency reflex amplitudes unlike in similarly treated wild-type mice

• mice treated with GR 103691 exhibit shorter monosynaptic than longer-latency reflex amplitude unlike in wild-type mice

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
restless legs syndrome		DOID:0050425	OMIM:PS102300	J:96803

Genotype
MGI:4839961

hm3

• Allelic Composition: Drd3^tm1Dac/Drd3^tm1Dac
• Genetic Background: involves: 129S4/SvJae

behavior/neurological

decreased alcohol consumption ( J:107932 )

• during chronic ethanol treatment

abnormal behavioral response to addictive substance ( J:103704 )

• mice treated with cocaine exhibit reduced hyperactivity and increased head bobbing compared with similarly treated wild-type mice

enhanced behavioral response to alcohol ( J:107932 )

• after a single injection of ethanol, mice exhibit reduced onset time to sleep and longer sleep time compared with similarly treated wild-type mice

increased behavioral withdrawal response ( J:107932 )

• 3 and 6 hours after discontinuation of ethanol treatment, mice exhibit increased levels of withdrawal scores compared with similarly treated wild-type mice

• however, withdrawal scores at 12 and 18 hours are normal

head bobbing ( J:103704 )

• cocaine-treated mice exhibit increased head bobbing compared with similarly treated wild-type mice

decreased locomotor activity ( J:103704 )

• 5 days after cocaine treatment, mice exhibit less induced locomotion compared with similarly treated wild-type mice

Genotype
MGI:3614420

hm4

• Allelic Composition: Drd3^tm1Dac/Drd3^tm1Dac
• Genetic Background: involves: 129S4/SvJae * C57BL/6

behavior/neurological

impaired behavioral response to morphine ( J:103701 )

• mice exhibit decreased morphine-induced conditioned place preference (CPP) compared with similarly treated wild-type mice

• BP 897-treated mice fail to exhibit a decrease in morphine-induced CPP compared with similarly treated wild-type mice

impaired conditioned place preference behavior ( J:103701 )

• mice exhibit decreased morphine-induced conditioned place preference (CPP) compared with similarly treated wild-type mice

• BP 897-treated mice fail to exhibit a decrease in morphine-induced CPP compared with similarly treated wild-type mice

increased exploration in new environment ( J:32128 )

• homozygotes exhibit increased exploratory behavior in the open field test

• in contrast, normal gait, coordination and primitive reflexes remain intact

decreased grooming behavior ( J:32128 )

• homozygotes tend to show reduced grooming behavior with a delayed onset; however, these differences are not statistically significant relative to wild-type grooming behavior

hyperactivity ( J:32128 )

• in the open field test, homozygotes display hyperactivity, as shown by a 57% increase in locomotor activity (crossings) relative to wild-type mice

increased vertical activity ( J:32128 )

• in the open field test, homozygotes display 93% more rearing episodes than wild-type mice

cardiovascular system

decreased systemic arterial systolic blood pressure ( J:115136 )

• systolic blood pressure in losartan-treated mice remains depressed longer than similarly treated wild-type mice

increased systemic arterial diastolic blood pressure ( J:115136 )

• after anesthesia with pentobarbital

increased systemic arterial systolic blood pressure ( J:115136 )

• after anesthesia with pentobarbital

homeostasis/metabolism

decreased urine sodium level ( J:115136 )

• after acute saline loading

increased renin activity ( J:115136 )

• in the kidneys

abnormal physiological response to xenobiotic ( J:115136 )

• systolic blood pressure in losartan-treated mice remains depressed longer than similarly treated wild-type mice

renal/urinary system

decreased urine sodium level ( J:115136 )

• after acute saline loading

decreased urine flow rate ( J:115136 )

• after acute saline loading, urine flow is decreased compared to in wild-type mice

Genotype
MGI:4839942

hm5

• Allelic Composition: Drd3^tm1Dac/Drd3^tm1Dac
• Genetic Background: involves: 129S4/SvJae * C57BL/6J

behavior/neurological

decreased anxiety-related response ( J:44685 )

• mice exhibit more time in the center of the open field and with the open arms of an elevated plus maze compared with wild-type mice

Genotype
MGI:3614421

ht6

• Allelic Composition: Drd3^tm1Dac/Drd3⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6

behavior/neurological

increased exploration in new environment ( J:32128 )

• heterozygotes exhibit increased exploratory behavior in the open field, with a degree of hyperactivity comparable to than of homozygotes during the last 11 min of a 15-min test

decreased grooming behavior ( J:32128 )

• heterozygotes tend to show reduced grooming behavior with a delayed onset; however, these differences are not statistically significant relative to wild-type grooming behavior

abnormal locomotor activation ( J:96229 )

• during the first part of the test (increased early and decreased late) unlike wild-type mice

hyperactivity ( J:32128 , J:96229 )

• in the open field test, heterozygotes display hyperactivity, as shown by a 38% increase in locomotor activity (crossings) relative to wild-type mice (J:32128)

• during the first part of the test (J:96229)

increased vertical activity ( J:32128 , J:96229 )

• in the open field test, heterozygotes display 77% more rearing episodes than wild-type mice (J:32128)

• during the first part of the test (J:96229)

cardiovascular system

increased systemic arterial diastolic blood pressure ( J:115136 )

• after anesthesia with pentobarbital

increased systemic arterial systolic blood pressure ( J:115136 )

• after anesthesia with pentobarbital

homeostasis/metabolism

increased renin activity ( J:115136 )

Genotype
MGI:4839943

ht7

• Allelic Composition: Drd3^tm1Dac/Drd3⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6J

behavior/neurological

decreased anxiety-related response ( J:44685 )

• mice exhibit moderately more time in the center of the open field and with the open arms of an elevated plus maze compared with wild-type mice

Genotype
MGI:4839981

cx8

• Allelic Composition: Drd2^tm1Ebo/Drd2^tm1Ebo; Drd3^tm1Dac/Drd3^tm1Dac
• Genetic Background: involves: 129S2/SvPas * 129S4/SvJae * C57BL/6

behavior/neurological

abnormal behavior ( J:96229 )

• in a non-selective attention test, mice exhibit lower scanning times (measured by duration of individual rearing episodes) during the first part of the test compared with wild-type mice

abnormal emotion/affect behavior ( J:96229 )

• mice exhibit a reduction in defecation score, as an emotional index, compared with wild-type mice

decreased vertical activity ( J:96229 )

• frequency and duration

decreased locomotor activity ( J:96229 )

digestive/alimentary system

abnormal defecation ( J:96229 )

• mice exhibit a reduction in defecation score, as an emotional index, compared with wild-type mice

Genotype
MGI:4839955

cx9

• Allelic Composition: Drd1^tm1Jcd/Drd1^tm1Jcd; Drd3^tm1Dac/Drd3^tm1Dac
• Genetic Background: involves: 129S4/SvJae * C57BL/6

behavior/neurological

behavior/neurological phenotype ( J:102600 )

N • mice exhibit normal numbers of entries into the open arms of an elevated plus maze compared with wild-type mice

abnormal spatial learning ( J:102600 )

• in a Morris water maze

behavioral despair ( J:102600 )

• mice tend to float in the water instead of swimming compared with wild-type mice

increased thigmotaxis ( J:102600 )

• in the water

impaired coordination ( J:102600 )

• compared with wild-type mice

impaired swimming ( J:102600 )

• mice exhibit lower swim speed compared with wild-type mice

decreased vertical activity ( J:102600 )

decreased locomotor activity ( J:102600 )

• in an open field compared with wild-type mice

growth/size/body

decreased body size ( J:102600 )