Phenotypes associated with this allele

• Allele Symbol: Alox5ap^tm1Jdm
• Allele Name: targeted mutation 1, John D McNeish
• Allele ID: MGI:1857167
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Alox5ap^tm1Jdm/Alox5ap^tm1Jdm	DBA/1LacJ-Alox5ap^tm1Jdm	MGI:3589824
ht2	Alox5ap^tm1Jdm/Alox5ap^+	DBA/1LacJ-Alox5ap^tm1Jdm	MGI:3589825

Genotype
MGI:3589824

hm1

• Allelic Composition: Alox5ap^tm1Jdm/Alox5ap^tm1Jdm
• Genetic Background: DBA/1LacJ-Alox5ap^tm1Jdm

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:100736 )

N • Background Sensitivity: the magnitude of leukotriene inhibition in DBA/1LacJ mice is less than that observed in the 129 strain

decreased susceptibility to induced arthritis ( J:100736 )

• at 21-56 days, homozygotes exhibit reduced severity of collagen-induced arthritis, with a ~73% decrease in total disease score relative to wild-type mice

• reduced severity is maintained even after injection of IL-1, which normally increases disease severity, despite normal serum anti-collagen antibody levels

decreased inflammatory response ( J:100736 )

• homozygotes display reduced acute and chronic inflammatory responses

skeleton

decreased susceptibility to induced arthritis ( J:100736 )

• at 21-56 days, homozygotes exhibit reduced severity of collagen-induced arthritis, with a ~73% decrease in total disease score relative to wild-type mice

• reduced severity is maintained even after injection of IL-1, which normally increases disease severity, despite normal serum anti-collagen antibody levels

homeostasis/metabolism

abnormal leukotriene level ( J:100736 )

• homozygotes are viable, fertile and phenotypically normal but fail to produce peptido-leukotrienes following zymosan-induced peritonitis

• as a result, homozygotes show ~50% reduction in plasma protein extravasation in the peritoneal cavity in response to zymosan-injection

• unexpectedly, 12(S)-hydroxyeicosatetraenoic acid (HETE) levels are slightly reduced

• at 30 min after i.p. zymosan injection, homozygotes produce no detectable levels of LTC4 or LTE4

• at 3 hrs after i.p. zymosan injection, homozygotes produce no detectable levels of LTB4

abnormal prostaglandin level ( J:100736 )

• homozygotes exhibit high levels of prostaglandins following zymosan-induced peritonitis

Genotype
MGI:3589825

ht2

• Allelic Composition: Alox5ap^tm1Jdm/Alox5ap⁺
• Genetic Background: DBA/1LacJ-Alox5ap^tm1Jdm

immune system

immune system phenotype ( J:100736 )

N • Background Sensitivity: the magnitude of leukotriene inhibition in DBA/1LacJ mice is less than that observed in the 129 strain

decreased susceptibility to induced arthritis ( J:100736 )

• at 21-56 days, heterozygotes exhibit reduced severity of collagen-induced arthritis, with a ~37% decrease in total disease score relative to wild-type mice

• reduced severity is maintained even after injection of IL-1, despite normal serum anti-collagen antibody levels

• similar to wild-type, affected heterozygotes display extensive leukocyte infiltration of synovial connective tissue, plasma protein extravasation into the synovial cavity, and variable degrees of articular cartilage damage

skeleton

decreased susceptibility to induced arthritis ( J:100736 )

• at 21-56 days, heterozygotes exhibit reduced severity of collagen-induced arthritis, with a ~37% decrease in total disease score relative to wild-type mice

• reduced severity is maintained even after injection of IL-1, despite normal serum anti-collagen antibody levels

• similar to wild-type, affected heterozygotes display extensive leukocyte infiltration of synovial connective tissue, plasma protein extravasation into the synovial cavity, and variable degrees of articular cartilage damage

homeostasis/metabolism

decreased leukotriene level ( J:100736 )

• zymosan-injected heterozygotes display a slight reduction in LTE4 levels, mean of 74% of wild-type