About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Il12btm1Jm
targeted mutation 1, Jeanne Magram
MGI:1857201
Summary 10 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Il12btm1Jm/Il12btm1Jm 129S1/Sv-Il12btm1Jm MGI:3691135
hm2
 		Il12btm1Jm/Il12btm1Jm B6.129S1-Il12btm1Jm/J MGI:3691133
hm3
 		Il12btm1Jm/Il12btm1Jm involves: 129S1/Sv MGI:4459518
hm4
 		Il12btm1Jm/Il12btm1Jm involves: 129S1/SvImJ MGI:3784502
cn5
 		Il12btm1Jm/Il12btm1Jm
Stat3tm2Aki/Stat3tm2Aki
Lyz2tm1(cre)Ifo/Lyz2+ 		involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6 MGI:3771395
cx6
 		Il12atm1Jm/Il12atm1Jm
Il12btm1Jm/Il12btm1Jm 		B6.129S1-Il12atm1Jm Il12btm1Jm MGI:3784444
cx7
 		Il10tm1Cgn/Il10tm1Cgn
Il12btm1Jm/Il12btm1Jm 		involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6 MGI:3851989
cx8
 		Il12btm1Jm/Il12btm1Jm
Il18tm1Aki/Il18tm1Aki 		involves: 129P2/OlaHsd * 129S1/SvImJ MGI:3784501
cx9
 		Il12btm1Jm/Il12btm1Jm
Rag2tm1Fwa/Rag2tm1Fwa 		involves: 129S/SvEv * 129S1/Sv MGI:3817879
cx10
 		Il12btm1Jm/Il12btm1Jm
Tnftm2Gkl/Tnf+ 		involves: 129S/SvEv * 129S1/Sv * C57BL/6J MGI:3629590


Genotype
MGI:3691135
hm1
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Genetic
Background		 129S1/Sv-Il12btm1Jm
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (33 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal CD4-positive, alpha-beta T cell physiology ( J:34164 )
• 75 days after infection Ifng mRNA expression in CD4+ cells is more than 50-fold lower than in wild-type
• 75 days after infection Il4 mRNA expression in CD4+ cells is 100-fold higher than in wild-type
• draining lymph node cells in L. major infected mice produce significantly more IL4 compared to IFNG in contrast to wild-type mice which express more IFNG than IL4
decreased susceptibility to type IV hypersensitivity reaction ( J:34164 )
• do not show any delayed type hypersensitivity response to application of Leishmania antigen to the uninfected footpad 41 days after initial infection with L. major
increased susceptibility to parasitic infection ( J:34164 )
• develop large lesions unlike wild-type 129S1/Sv mice after infection with Leishmania major
• 75 days after infection with L. major parasite numbers in the draining lymph nodes are increased 400-fold compared to wild-type mice

hematopoietic system
abnormal CD4-positive, alpha-beta T cell physiology ( J:34164 )
• 75 days after infection Ifng mRNA expression in CD4+ cells is more than 50-fold lower than in wild-type
• 75 days after infection Il4 mRNA expression in CD4+ cells is 100-fold higher than in wild-type
• draining lymph node cells in L. major infected mice produce significantly more IL4 compared to IFNG in contrast to wild-type mice which express more IFNG than IL4




Genotype
MGI:3691133
hm2
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Genetic
Background		 B6.129S1-Il12btm1Jm/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (33 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
increased susceptibility to xenobiotic induced morbidity/mortality ( J:91927 )
• increase in mortality following a single dose of N-methyl-N-nitrosourea (MNU) compared to similarly treated wild-type controls

growth/size/body
alveolar process atrophy ( J:147935 )
• 3 fold increase in alveolar bone loss at 30 weeks relative to mice at 6 and 16 weeks

immune system
abnormal granulocyte physiology ( J:111568 )
• fewer than expected granulocytes in the dermis of carcinogen treated skin
abnormal lymphocyte physiology ( J:120806 )
• lymph node cells from keyhole limpet hemocyanin (KLH) immunized mice are severely impaired in their ability to produce IFNG when cultured with KLH; however, their production of IL4 is increased
abnormal NK cell physiology ( J:120806 )
• mean NK lytic activity is about 66% of controls; however when cultured with IL2 lytic activity is similar to controls
abnormal CD8-positive, alpha-beta T cell physiology ( J:111568 )
• dramatic increase in the number of cytotoxic CD8+ cells in the dermis and epidermis of carcinogen treated skin
abnormal macrophage physiology ( J:111568 )
• fewer than expected macrophages in the dermis of carcinogen treated skin
decreased circulating interferon-gamma level ( J:120806 )
• serum levels of IFNG in LPS-injected mice are only 17 +/- 5% of LPS-induced controls
decreased susceptibility to type IV hypersensitivity reaction ( J:120806 )
• specific foot pad swelling 48 hours after challenge with methylated bovine serum albumin is inhibited by 47 +/- 3% compared to wild-type mice; however, cytotoxic T lymphocyte responses are similar to wild-type
decreased interleukin-17 secretion ( J:111568 )
• barely detectable levels of IL17 in the skin after carcinogen treatment unlike wild-type mice which express high levels of IL17
increased susceptibility to bacterial infection ( J:120806 )
• vaccination does not produce a protective response to M. tuberculosis

neoplasm
decreased incidence of tumors by chemical induction ( J:111568 )
• resistant to induction of papillomas compared to wild-type mice after treatment with DMBA and TPA in a 2 step skin carcinogenesis protocol
• however, there is an increase in the incidence of tumors following intradermal challenge with PDV squamous carcinoma cells

homeostasis/metabolism
decreased circulating interferon-gamma level ( J:120806 )
• serum levels of IFNG in LPS-injected mice are only 17 +/- 5% of LPS-induced controls
increased susceptibility to xenobiotic induced morbidity/mortality ( J:91927 )
• increase in mortality following a single dose of N-methyl-N-nitrosourea (MNU) compared to similarly treated wild-type controls
decreased incidence of tumors by chemical induction ( J:111568 )
• resistant to induction of papillomas compared to wild-type mice after treatment with DMBA and TPA in a 2 step skin carcinogenesis protocol
• however, there is an increase in the incidence of tumors following intradermal challenge with PDV squamous carcinoma cells

craniofacial
alveolar process atrophy ( J:147935 )
• 3 fold increase in alveolar bone loss at 30 weeks relative to mice at 6 and 16 weeks

skeleton
alveolar process atrophy ( J:147935 )
• 3 fold increase in alveolar bone loss at 30 weeks relative to mice at 6 and 16 weeks

hematopoietic system
abnormal granulocyte physiology ( J:111568 )
• fewer than expected granulocytes in the dermis of carcinogen treated skin
abnormal lymphocyte physiology ( J:120806 )
• lymph node cells from keyhole limpet hemocyanin (KLH) immunized mice are severely impaired in their ability to produce IFNG when cultured with KLH; however, their production of IL4 is increased
abnormal NK cell physiology ( J:120806 )
• mean NK lytic activity is about 66% of controls; however when cultured with IL2 lytic activity is similar to controls
abnormal CD8-positive, alpha-beta T cell physiology ( J:111568 )
• dramatic increase in the number of cytotoxic CD8+ cells in the dermis and epidermis of carcinogen treated skin
abnormal macrophage physiology ( J:111568 )
• fewer than expected macrophages in the dermis of carcinogen treated skin




Genotype
MGI:4459518
hm3
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Genetic
Background		 involves: 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (33 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
increased susceptibility to bacterial infection induced morbidity/mortality ( J:161073 )
• mice died before day 12 after inoculation with C. rodentium

immune system
increased susceptibility to bacterial infection induced morbidity/mortality ( J:161073 )
• mice died before day 12 after inoculation with C. rodentium




Genotype
MGI:3784502
hm4
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Genetic
Background		 involves: 129S1/SvImJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (33 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
impaired natural killer cell mediated cytotoxicity ( J:46514 )
• the killing activity of splenic NK cells is about 2/3rds that of wild-type mice as measured by in vitro co-culturing with YAC-1 target cells
• normal level of target cell killing can be restored by in vivo pretreatment with IL-18 for two days or the addition of IL-18 to the co-culture
abnormal T-helper 1 physiology ( J:46514 )
• in vivo Th1 cell response is impaired as indicated by the low amounts of IFN-gamma produced by T cells in response to M. bovis antigens
decreased interferon-gamma secretion ( J:46514 )
• splenic T cells produce about 1/4th the amount of IFN-gamma as T cells from wild-type mice 14 days after injection with heat killed M. Bovis

hematopoietic system
impaired natural killer cell mediated cytotoxicity ( J:46514 )
• the killing activity of splenic NK cells is about 2/3rds that of wild-type mice as measured by in vitro co-culturing with YAC-1 target cells
• normal level of target cell killing can be restored by in vivo pretreatment with IL-18 for two days or the addition of IL-18 to the co-culture
abnormal T-helper 1 physiology ( J:46514 )
• in vivo Th1 cell response is impaired as indicated by the low amounts of IFN-gamma produced by T cells in response to M. bovis antigens




Genotype
MGI:3771395
cn5
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Stat3tm2Aki/Stat3tm2Aki
Lyz2tm1(cre)Ifo/Lyz2+
Genetic
Background		 involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (33 available)
Lyz2tm1(cre)Ifo mutation (14 available); any Lyz2 mutation (40 available)
Stat3tm2Aki mutation (1 available); any Stat3 mutation (72 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
digestive/alimentary phenotype ( J:83280 )
N
• mice exhibit normal Th1 responses and do not develop colitis, unlike Stat3 null mice




Genotype
MGI:3784444
cx6
Allelic
Composition		 Il12atm1Jm/Il12atm1Jm
Il12btm1Jm/Il12btm1Jm
Genetic
Background		 B6.129S1-Il12atm1Jm Il12btm1Jm
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12atm1Jm mutation (2 available); any Il12a mutation (35 available)
Il12btm1Jm mutation (4 available); any Il12b mutation (33 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
impaired natural killer cell mediated cytotoxicity ( J:125611 )
• following infection with Leishmania infantum

hematopoietic system
impaired natural killer cell mediated cytotoxicity ( J:125611 )
• following infection with Leishmania infantum




Genotype
MGI:3851989
cx7
Allelic
Composition		 Il10tm1Cgn/Il10tm1Cgn
Il12btm1Jm/Il12btm1Jm
Genetic
Background		 involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il10tm1Cgn mutation (15 available); any Il10 mutation (45 available)
Il12btm1Jm mutation (4 available); any Il12b mutation (33 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal T-helper 2 cell differentiation ( J:111712 )
• mice exhibit enhanced Th2 responses after infection with S. mansoni eggs compared to controls

immune system
abnormal T-helper 2 cell differentiation ( J:111712 )
• mice exhibit enhanced Th2 responses after infection with S. mansoni eggs compared to controls
increased interleukin-13 secretion ( J:111712 )
• there is a 200- to 250-fold increase in IL-13 mRNA present in the lungs after infection with S. mansoni eggs




Genotype
MGI:3784501
cx8
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Il18tm1Aki/Il18tm1Aki
Genetic
Background		 involves: 129P2/OlaHsd * 129S1/SvImJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (33 available)
Il18tm1Aki mutation (3 available); any Il18 mutation (32 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
impaired natural killer cell mediated cytotoxicity ( J:46514 )
• the killing ability of splenic NK cells is severely impaired with significantly less activity occurring than NK cells from Il18tm1Aki or Il12btm1Jm homozygotes
abnormal T-helper 1 physiology ( J:46514 )
• in vivo Th1 cell response is impaired as indicated by the low amounts of IFN-gamma produced by T cells in response to M. bovis antigens
decreased interferon-gamma secretion ( J:46514 )
• splenic T cells produce 12-fold lower amounts of IFN-gamma as T cells from wild-type mice 14 days after injection with heat killed M. Bovis

hematopoietic system
impaired natural killer cell mediated cytotoxicity ( J:46514 )
• the killing ability of splenic NK cells is severely impaired with significantly less activity occurring than NK cells from Il18tm1Aki or Il12btm1Jm homozygotes
abnormal T-helper 1 physiology ( J:46514 )
• in vivo Th1 cell response is impaired as indicated by the low amounts of IFN-gamma produced by T cells in response to M. bovis antigens




Genotype
MGI:3817879
cx9
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Rag2tm1Fwa/Rag2tm1Fwa
Genetic
Background		 involves: 129S/SvEv * 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (33 available)
Rag2tm1Fwa mutation (45 available); any Rag2 mutation (119 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal chemokine secretion ( J:140483 )
• chemokines KC and MIP-2 are increased in NK cells in infected lungs
abnormal interferon secretion ( J:140483 )
• splenocytes produce minimal levels of Ifng in response to mycobacterial stimulation, in contrast to response of Rag2-null cells
lung inflammation ( J:140483 )
• at 28 days post-infection (p.i.), lungs display exacerbated pulmonary inflammatory pathology relative to Rag2-null animals; lesions contain significant numbers of granulocytes compared to Il2rg, Rag2-double null mice
increased susceptibility to bacterial infection ( J:140483 )
• mice succumb rapidly to infection compared to Rag2-null mice
• in lungs and spleens of M. tuberculosis-infected mice at 28 days post-infection (p.i.), bacterial loads are 0.5-1.0 log higher than in infected Rag2-null mice

respiratory system
lung inflammation ( J:140483 )
• at 28 days post-infection (p.i.), lungs display exacerbated pulmonary inflammatory pathology relative to Rag2-null animals; lesions contain significant numbers of granulocytes compared to Il2rg, Rag2-double null mice




Genotype
MGI:3629590
cx10
Allelic
Composition		 Il12btm1Jm/Il12btm1Jm
Tnftm2Gkl/Tnf+
Genetic
Background		 involves: 129S/SvEv * 129S1/Sv * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il12btm1Jm mutation (4 available); any Il12b mutation (33 available)
Tnftm2Gkl mutation (1 available); any Tnf mutation (48 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
small intestinal inflammation ( J:108572 )
• mice display delayed development and attenuation of inflammatory bowel disease

digestive/alimentary system
small intestinal inflammation ( J:108572 )
• mice display delayed development and attenuation of inflammatory bowel disease




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory