Analysis Tools
mortality/aging
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• homozygotes die between E10.0 and E11.0
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embryo
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• at E9.5, mutant embryos are only partially turned into the fetal position
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• at E8.5, homozygotes display an abnormal curvature
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• at E9.5, the posterior trunk of mutant embryos is deformed and only 1/3 of E9.0 wild-type length
• although delayed, the anterior region (including heads, branchial arches and heart) appears relatively unaffected
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• at E8.5, homozygotes display a slightly truncated anterior-posterior axis
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• at E9.5, mutant embryos are developmentally retarded and equivalent in size to E9.0 wild-type embryos
• by E10.5, mutant embryos are equivalent in size to E9.5 wild-type embryos
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• at E9.5, homozygotes display a deficit in paraxial mesoderm in the region flanking the neural tube
• mesenchymeal cells, normally found on the dorsal side of somites are absent
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• at E9.5, homozygotes exhibit kinked neural tubes in the posterior trunk region
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• at E9.5, mutant embryos lack somites in the truncated posterior regions; however, normal somite numbers (7-10 pairs) are noted anteriorly
• at E8.0-E8.5, most mutant embryos exhibit normal numbers of somites relative to wild-type embryos (4-13 pairs)
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• at E9.5, mutant yolk sacs display abnormal blood vessels, with large numbers of blood cells leaking into the exocoelomic space
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• at E9.5, the extraembryonic mesodermal layer is reduced and separated from the extraembryonic endoderm
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growth/size/body
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• at E9.5, mutant embryos are developmentally retarded and equivalent in size to E9.0 wild-type embryos
• by E10.5, mutant embryos are equivalent in size to E9.5 wild-type embryos
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nervous system
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• at E9.5, homozygotes exhibit kinked neural tubes in the posterior trunk region
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cardiovascular system
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• all homozygotes form hearts and a vascular system; however, primitive blood vessels are distended and leaky
• at E9.5, homozygotes show vascular defects both in the embryo and in extraembryonic vasculature, with fewer blood cells present in the heart and blood vessels
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• at E9.5, the mutant dorsal aortae are not fully closed, with leakage of primitive blood cells into the mesoderm-deficient space
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• at E9.5, mutant yolk sacs display abnormal blood vessels, with large numbers of blood cells leaking into the exocoelomic space
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cellular
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• mutant embryonic fibroblasts are able to assemble fibronectin matrix, form focal contacts, and migrate on fibronectin
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