Phenotypes associated with this allele

• Allele Symbol: Mos^tm1Ev
• Allele Name: targeted mutation 1, Martin L Evans
• Allele ID: MGI:1857218
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mos^tm1Ev/Mos^tm1Ev	involves: 129S/SvEv	MGI:2183304

Genotype
MGI:2183304

hm1

• Allelic Composition: Mos^tm1Ev/Mos^tm1Ev
• Genetic Background: involves: 129S/SvEv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

ovary cyst ( J:19118 )

♀ • ovarian cysts are found in 5 mutant females but not in wild-type (0/19) or heterozygous animals (0/10); incidence is high (4/7) in females older than 6 months

♀ • cysts are serous, bilateral, multilocular in older mice and sometimes infiltrated with blood; smaller cysts are predominantly composed of epithelial tissue, while larger cysts (2/5) contain tissue types typical of a teratoma

reproductive system

reproductive system phenotype ( J:77797 )

♂N • normal sperm count and spermatogenesis

abnormal polar body morphology ( J:19118 )

♀ • eggs isolated 18 hours after human chorionic gonadotropin treatment display 2 polar bodies and no pronuclei while egg from wild-type littermates have only one polar body

abnormal female meiosis ( J:19118 )

♀ • eggs failed to arrest during meiosis, leading to spontaneous parthenogenetic activation (progress through meiosis II, germinal vesicle breakdown, extrusion of polar bodies, and cleavage)

abnormal meiotic spindle morphology ( J:19118 , J:110272 )

• eggs isolated 18 hours after human chorionic gonadotropin treatment display 2 polar bodies and no pronuclei while egg from wild-type littermates have only one polar body (J:19118)

• ~40% of mutant eggs maintained in culture develop a pronucleus indicating parthenogenetic development; 30% of these cleave into 2 nucleated cells and occasionally to 4 cells (J:19118)

• mutant eggs that fail to cleave undergo cytoplasmic fragmentation which is not seen in wild-type eggs (J:19118)

• in Mos-deficient oocytes the meiotic spindle does not migrate in contrast to wild-type; rather, it elongates dramatically followed by cortical polarization (J:110272)

ovary cyst ( J:19118 )

♀ • ovarian cysts are found in 5 mutant females but not in wild-type (0/19) or heterozygous animals (0/10); incidence is high (4/7) in females older than 6 months

♀ • cysts are serous, bilateral, multilocular in older mice and sometimes infiltrated with blood; smaller cysts are predominantly composed of epithelial tissue, while larger cysts (2/5) contain tissue types typical of a teratoma

reduced female fertility ( J:19118 )

♀ • females but not males have reduced fertility

decreased litter size ( J:19118 )

• females have litters with average size of 2 pups, compared to wild-type and heterozygous females which have ~7 pups/litter

cellular

abnormal polar body morphology ( J:19118 )

♀ • eggs isolated 18 hours after human chorionic gonadotropin treatment display 2 polar bodies and no pronuclei while egg from wild-type littermates have only one polar body

abnormal female meiosis ( J:19118 )

♀ • eggs failed to arrest during meiosis, leading to spontaneous parthenogenetic activation (progress through meiosis II, germinal vesicle breakdown, extrusion of polar bodies, and cleavage)

abnormal meiotic spindle morphology ( J:19118 , J:110272 )

• eggs isolated 18 hours after human chorionic gonadotropin treatment display 2 polar bodies and no pronuclei while egg from wild-type littermates have only one polar body (J:19118)

• ~40% of mutant eggs maintained in culture develop a pronucleus indicating parthenogenetic development; 30% of these cleave into 2 nucleated cells and occasionally to 4 cells (J:19118)

• mutant eggs that fail to cleave undergo cytoplasmic fragmentation which is not seen in wild-type eggs (J:19118)

• in Mos-deficient oocytes the meiotic spindle does not migrate in contrast to wild-type; rather, it elongates dramatically followed by cortical polarization (J:110272)

growth/size/body

ovary cyst ( J:19118 )

♀ • ovarian cysts are found in 5 mutant females but not in wild-type (0/19) or heterozygous animals (0/10); incidence is high (4/7) in females older than 6 months

♀ • cysts are serous, bilateral, multilocular in older mice and sometimes infiltrated with blood; smaller cysts are predominantly composed of epithelial tissue, while larger cysts (2/5) contain tissue types typical of a teratoma