Phenotypes associated with this allele

• Allele Symbol: Ncam1^tm1Cgn
• Allele Name: targeted mutation 1, University of Cologne
• Allele ID: MGI:1857222
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ncam1^tm1Cgn/Ncam1^tm1Cgn	B6.129P2-Ncam1^tm1Cgn	MGI:3762776
hm2	Ncam1^tm1Cgn/Ncam1^tm1Cgn	involves: 129P2/OlaHsd	MGI:2182567
hm3	Ncam1^tm1Cgn/Ncam1^tm1Cgn	involves: 129P2/OlaHsd * C57BL/6J	MGI:6383926
hm4	Ncam1^tm1Cgn/Ncam1^tm1Cgn	involves: C57BL/6J	MGI:3574807
cx5	Mical1^tm1.2Past/Mical1^+ Ncam1^tm1Cgn/Ncam1^+	involves: 129 * 129P2/OlaHsd	MGI:7628684
cx6	Mag^tm1Mtg/Mag^tm1Mtg Ncam1^tm1Cgn/Ncam1^tm1Cgn	involves: 129P2/OlaHsd * 129S2/SvPas	MGI:4355933

Genotype
MGI:3762776

hm1

• Allelic Composition: Ncam1^tm1Cgn/Ncam1^tm1Cgn
• Genetic Background: B6.129P2-Ncam1^tm1Cgn

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal neuron differentiation ( J:126687 )

• more neurospheres differentiate into GFAP+ cells when exposed to PDGF (44.1+/-7.5% compared to 33.0+/-4.5% of wild-type neurospheres)

abnormal CNS glial cell morphology ( J:126687 )

• the number of GFAP+ cells is increased along the rostral migratory stream but these cells are not within the stream nor directed to the olfactory bulb

cellular

abnormal neuron differentiation ( J:126687 )

• more neurospheres differentiate into GFAP+ cells when exposed to PDGF (44.1+/-7.5% compared to 33.0+/-4.5% of wild-type neurospheres)

Genotype
MGI:2182567

hm2

• Allelic Composition: Ncam1^tm1Cgn/Ncam1^tm1Cgn
• Genetic Background: involves: 129P2/OlaHsd

behavior/neurological

abnormal spatial learning ( J:16590 )

• spatial learning impairment demonstrated in the Morris water maze test

nervous system

decreased brain size ( J:16590 )

small olfactory bulb ( J:16590 )

Genotype
MGI:6383926

hm3

• Allelic Composition: Ncam1^tm1Cgn/Ncam1^tm1Cgn
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

vision/eye

decreased retina ganglion cell number ( J:235028 )

• at 18 months

• however, the number of optic nerve axons is normal at 2 and 18 months

increased retina ganglion cell number ( J:235028 )

• at 2 months

• however, the number of optic nerve axons is normal at 2 and 18 months

thick retina ganglion layer ( J:235028 )

• in middle and peripheral regions at 2 months

thin retina inner nuclear layer ( J:235028 )

• at 18 months

thick retina inner plexiform layer ( J:235028 )

• increased at 2 months

thin retina outer nuclear layer ( J:235028 )

• loss of one third of thickness in the central areas at 18 months

thin retina outer plexiform layer ( J:235028 )

• at 18 months

abnormal total retina thickness ( J:235028 )

• increased retinal thickness only in the peripheral temporal region at 2 months

• reduced retinal thickness in the central areas at 18 months

• however, mice exhibit normal thickness in the inner nuclear layer, outer plexiform layer and outer nuclear layer at 2 months

abnormal rod electrophysiology ( J:235028 )

• a-wave Vmax is more than double wild-type before falling to normal levels by 10 months and older

• b-wave V-max is more than double at 2 months then falls to normal levels by 10 months or older with premature loss of light sensitivity at 18 months

• however, mice exhibit normal retinal sensitivity and b-wave to a-wave ratio

abnormal vision ( J:235028 )

• premature loss of visual detection

• no ability to detect pattern at any age

decreased visual acuity ( J:235028 )

• premature

nervous system

decreased retina ganglion cell number ( J:235028 )

• at 18 months

• however, the number of optic nerve axons is normal at 2 and 18 months

increased retina ganglion cell number ( J:235028 )

• at 2 months

• however, the number of optic nerve axons is normal at 2 and 18 months

Genotype
MGI:3574807

hm4

• Allelic Composition: Ncam1^tm1Cgn/Ncam1^tm1Cgn
• Genetic Background: involves: C57BL/6J

behavior/neurological

abnormal circadian behavior period ( J:96708 )

• in 50% of mutant in DD conditions aberrant activity rhythms make it impossible to determine a period

• these mutants also had a longer active period in DD conditions

shortened circadian behavior period ( J:96708 )

• the circadian period in DD conditions is significantly shorter in 50% of mutants, 23.7 hours compared to 23.98 hours in wild-type littermates

abnormal circadian behavior phase ( J:96708 )

• daily onset and offset of activity are irregular or lost

Genotype
MGI:7628684

cx5

• Allelic Composition: Mical1^tm1.2Past/Mical1⁺; Ncam1^tm1Cgn/Ncam1⁺
• Genetic Background: involves: 129 * 129P2/OlaHsd

nervous system

abnormal innervation ( J:240378 )

• mice exhibit an increase in the number of mossy fibers in the stratum pyramidale indicating that the characteristic lamination pattern of mossy fibers emanating from the dentate gyrus is altered

Genotype
MGI:4355933

cx6

• Allelic Composition: Mag^tm1Mtg/Mag^tm1Mtg; Ncam1^tm1Cgn/Ncam1^tm1Cgn
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas

nervous system

abnormal axon morphology ( J:78646 )

• at 4 weeks of age, onion bulb like structures are seen in the pectineus and quadriceps nerves

abnormal myelin sheath morphology ( J:78646 )

• at 8 weeks of age in single fiber preparations many fibers show signs of myelin degeneration

• degeneration is preferentially associated with the paranodal regions of the fibers

axon degeneration ( J:78646 )

• at 4 weeks of age, degenerating axons and degenerating myelin are seen in the femoral quadriceps nerve

• at 8 weeks of age the number of abnormal axons is increased compared to mice at 4 weeks of age