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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tgfb3tm1Doe
targeted mutation 1, Thomas Doetschman
MGI:1857259
Summary 15 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Tgfb3tm1Doe/Tgfb3tm1Doe either: (involves: 129 * 129P2/OlaHsd) or (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * CF-1) MGI:3041530
hm2
Tgfb3tm1Doe/Tgfb3tm1Doe involves: 129 * C57BL/6J * ICR * Swiss Webster MGI:5008407
hm3
Tgfb3tm1Doe/Tgfb3tm1Doe involves: 129P2/OlaHsd * C57BL/6 MGI:5008274
hm4
Tgfb3tm1Doe/Tgfb3tm1Doe involves: 129P2/OlaHsd * C57BL/6J MGI:7294492
ht5
Tgfb3tm1Doe/Tgfb3+ involves: 129P2/OlaHsd MGI:5008271
cx6
Tgfb1tm1Jmu/Tgfb1tm1Jmu
Tgfb3tm1Doe/Tgfb3tm1Doe
involves: 129 * BALB/c * C57BL/6J * Swiss Webster MGI:5008412
cx7
Tgfb1tm1Jmu/Tgfb1tm1Jmu
Tgfb3tm1Doe/Tgfb3+
involves: 129 * BALB/c * C57BL/6J * Swiss Webster MGI:5008410
cx8
Tgfb1tm1Jmu/Tgfb1tm1Jmu
Tgfb3tm1Doe/Tgfb3+
involves: 129 * C57BL/6J * ICR * Swiss Webster MGI:5008413
cx9
Tgfb1tm1Jmu/Tgfb1tm1Jmu
Tgfb3tm1Doe/Tgfb3tm1Doe
involves: 129 * C57BL/6J * ICR * Swiss Webster MGI:5008415
cx10
Tgfb2tm1Doe/Tgfb2+
Tgfb3tm1Doe/Tgfb3tm1Doe
involves: 129P2/OlaHsd MGI:5008265
cx11
Tgfb2tm1Doe/Tgfb2tm1Doe
Tgfb3tm1Doe/Tgfb3tm1Doe
involves: 129P2/OlaHsd MGI:5008266
cx12
Tgfb2tm1Doe/Tgfb2tm1Doe
Tgfb3tm1Doe/Tgfb3+
involves: 129P2/OlaHsd MGI:5008267
cx13
Tgfb2tm1Doe/Tgfb2+
Tgfb3tm1Doe/Tgfb3+
involves: 129P2/OlaHsd MGI:5008269
cx14
Tgfb1tm1Jmu/Tgfb1tm1Jmu
Tgfb3tm1Doe/Tgfb3tm1Doe
involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6J * Swiss Webster MGI:5008411
cx15
Tgfb1tm1Jmu/Tgfb1tm1Jmu
Tgfb3tm1Doe/Tgfb3+
involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6J * Swiss Webster MGI:5008408


Genotype
MGI:3041530
hm1
Allelic
Composition
Tgfb3tm1Doe/Tgfb3tm1Doe
Genetic
Background
either: (involves: 129 * 129P2/OlaHsd) or (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * CF-1)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfb3tm1Doe mutation (1 available); any Tgfb3 mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• all homozygotes die within 24 hrs after birth
• no homozygotes are recovered at 3 weeks of age, independent of genetic background

behavior/neurological
• homozygous mutant pups fail to suckle, as shown by absence of milk in their stomachs

craniofacial
• failure of palatal shelves to fuse appears to result from impaired adhesion of the apposing medial edge epithelia (MEE) and subsequent loss of the mid-line epithelial seam
• when fusion did occur, the MEE seam persisted in the mutant mice
• newborn homozygotes show a cleft palate phenotype of variable severity and type (anterior vs posterior) that is most severe on a C57BL/6-enriched background
• no associated cleft lip or gross abnormalities in cartilage, bone, brain, heart or other craniofacial structures (e.g. mandible) are observed
• Background Sensitivity: on a 129P2/OlaHsd x C57BL/6 background, 46.7% of homozygotes exhibit a complete cleft palate while 53.3% display only a posterior cleft
• Background Sensitivity: on a 129P2/OlaHsd x CF1 background, 89.3% homozygotes show a posterior cleft palate, 8.7% show anterior clefts (i.e. failure of fusion of primary and secondary palates), and ~2% exhibit complete clefts
• Background Sensitivity: on a 129/Sv x 129P2/OlaHsd background, all homozygotes show only a posterior cleft palate
• Background Sensitivity: on a 129P2/OlaHsd x CF1 background, 89.3% homozygotes show a posterior cleft palate, 8.7% show anterior clefts (i.e. failure of fusion of primary and secondary palates), and ~2% exhibit complete clefts

homeostasis/metabolism
• newborn pups become cyanotic shortly after birth
• newborn pups become dehydrated shortly after birth

respiratory system
• newborn homozygotes display an abnormal terminal airway system
• newborn pups exhibit gasping at ~4 hrs after birth

digestive/alimentary system
• failure of palatal shelves to fuse appears to result from impaired adhesion of the apposing medial edge epithelia (MEE) and subsequent loss of the mid-line epithelial seam
• when fusion did occur, the MEE seam persisted in the mutant mice
• newborn homozygotes show a cleft palate phenotype of variable severity and type (anterior vs posterior) that is most severe on a C57BL/6-enriched background
• no associated cleft lip or gross abnormalities in cartilage, bone, brain, heart or other craniofacial structures (e.g. mandible) are observed
• Background Sensitivity: on a 129P2/OlaHsd x C57BL/6 background, 46.7% of homozygotes exhibit a complete cleft palate while 53.3% display only a posterior cleft
• Background Sensitivity: on a 129P2/OlaHsd x CF1 background, 89.3% homozygotes show a posterior cleft palate, 8.7% show anterior clefts (i.e. failure of fusion of primary and secondary palates), and ~2% exhibit complete clefts
• Background Sensitivity: on a 129/Sv x 129P2/OlaHsd background, all homozygotes show only a posterior cleft palate
• Background Sensitivity: on a 129P2/OlaHsd x CF1 background, 89.3% homozygotes show a posterior cleft palate, 8.7% show anterior clefts (i.e. failure of fusion of primary and secondary palates), and ~2% exhibit complete clefts

growth/size/body
• failure of palatal shelves to fuse appears to result from impaired adhesion of the apposing medial edge epithelia (MEE) and subsequent loss of the mid-line epithelial seam
• when fusion did occur, the MEE seam persisted in the mutant mice
• newborn homozygotes show a cleft palate phenotype of variable severity and type (anterior vs posterior) that is most severe on a C57BL/6-enriched background
• no associated cleft lip or gross abnormalities in cartilage, bone, brain, heart or other craniofacial structures (e.g. mandible) are observed
• Background Sensitivity: on a 129P2/OlaHsd x C57BL/6 background, 46.7% of homozygotes exhibit a complete cleft palate while 53.3% display only a posterior cleft
• Background Sensitivity: on a 129P2/OlaHsd x CF1 background, 89.3% homozygotes show a posterior cleft palate, 8.7% show anterior clefts (i.e. failure of fusion of primary and secondary palates), and ~2% exhibit complete clefts
• Background Sensitivity: on a 129/Sv x 129P2/OlaHsd background, all homozygotes show only a posterior cleft palate
• Background Sensitivity: on a 129P2/OlaHsd x CF1 background, 89.3% homozygotes show a posterior cleft palate, 8.7% show anterior clefts (i.e. failure of fusion of primary and secondary palates), and ~2% exhibit complete clefts




Genotype
MGI:5008407
hm2
Allelic
Composition
Tgfb3tm1Doe/Tgfb3tm1Doe
Genetic
Background
involves: 129 * C57BL/6J * ICR * Swiss Webster
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfb3tm1Doe mutation (1 available); any Tgfb3 mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
craniofacial
• incomplete, posterior

respiratory system
N
• mice exhibit normal lung morphology

digestive/alimentary system
• incomplete, posterior

growth/size/body
• incomplete, posterior




Genotype
MGI:5008274
hm3
Allelic
Composition
Tgfb3tm1Doe/Tgfb3tm1Doe
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfb3tm1Doe mutation (1 available); any Tgfb3 mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• 2-fold in neurons of the substantia nigra pars compacta and ventral tegmental area
• at P0 in the substantia nigra pars compacta and ventral tegmental area

cellular
• 2-fold in neurons of the substantia nigra pars compacta and ventral tegmental area




Genotype
MGI:7294492
hm4
Allelic
Composition
Tgfb3tm1Doe/Tgfb3tm1Doe
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfb3tm1Doe mutation (1 available); any Tgfb3 mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
craniofacial
• at E14.5 palates are composed of a multilayer epithelium with trapped, flattened periderm between the layers
• shelves meet but form an inadequate and temporary fusion due to the trapped periderm

embryo
• periderm fails to undergo desquamation and persists in the palatal shelves at E14.5
• at E14.25 palatal periderm retains intact with a spherical-cobblestone appearance, does not dislodge, and remains tethered to the basal cells

digestive/alimentary system
• at E14.5 palates are composed of a multilayer epithelium with trapped, flattened periderm between the layers
• shelves meet but form an inadequate and temporary fusion due to the trapped periderm

growth/size/body
• at E14.5 palates are composed of a multilayer epithelium with trapped, flattened periderm between the layers
• shelves meet but form an inadequate and temporary fusion due to the trapped periderm




Genotype
MGI:5008271
ht5
Allelic
Composition
Tgfb3tm1Doe/Tgfb3+
Genetic
Background
involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfb3tm1Doe mutation (1 available); any Tgfb3 mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• mice exhibit reduced enterocyte apoptosis in the small intestine compared with wild-type mice
• however, enterocyte apoptosis in the colon is normal
• villus length is increased compared to in wild-type mice

cellular
• mice exhibit reduced enterocyte apoptosis in the small intestine compared with wild-type mice
• however, enterocyte apoptosis in the colon is normal




Genotype
MGI:5008412
cx6
Allelic
Composition
Tgfb1tm1Jmu/Tgfb1tm1Jmu
Tgfb3tm1Doe/Tgfb3tm1Doe
Genetic
Background
involves: 129 * BALB/c * C57BL/6J * Swiss Webster
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfb1tm1Jmu mutation (0 available); any Tgfb1 mutation (35 available)
Tgfb3tm1Doe mutation (1 available); any Tgfb3 mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• very few mice are born
• Background Sensitivity: fewer mice are born than on a background lacking BALB/c or containing ICR

cardiovascular system
• in all mice

nervous system
• in all mice




Genotype
MGI:5008410
cx7
Allelic
Composition
Tgfb1tm1Jmu/Tgfb1tm1Jmu
Tgfb3tm1Doe/Tgfb3+
Genetic
Background
involves: 129 * BALB/c * C57BL/6J * Swiss Webster
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfb1tm1Jmu mutation (0 available); any Tgfb1 mutation (35 available)
Tgfb3tm1Doe mutation (1 available); any Tgfb3 mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• most mice only survive 3 weeks before dying of widespread inflammation

cardiovascular system

nervous system




Genotype
MGI:5008413
cx8
Allelic
Composition
Tgfb1tm1Jmu/Tgfb1tm1Jmu
Tgfb3tm1Doe/Tgfb3+
Genetic
Background
involves: 129 * C57BL/6J * ICR * Swiss Webster
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfb1tm1Jmu mutation (0 available); any Tgfb1 mutation (35 available)
Tgfb3tm1Doe mutation (1 available); any Tgfb3 mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• most mice only survive 3 weeks before dying of widespread inflammation

cardiovascular system
• in newborn mice, brain vasculature exhibits cavitation compared to in control mice
• in all newborn mice
• less severe than in double homozygotes

nervous system
• in newborn mice, brain vasculature exhibits cavitation compared to in control mice
• in all newborn mice
• less severe than in double homozygotes
• in newborn mice




Genotype
MGI:5008415
cx9
Allelic
Composition
Tgfb1tm1Jmu/Tgfb1tm1Jmu
Tgfb3tm1Doe/Tgfb3tm1Doe
Genetic
Background
involves: 129 * C57BL/6J * ICR * Swiss Webster
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfb1tm1Jmu mutation (0 available); any Tgfb1 mutation (35 available)
Tgfb3tm1Doe mutation (1 available); any Tgfb3 mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
N
• Background Sensitivity: more mice are born on a background containing ICR than on a background containing BALB/c or lacking both ICR and BALB/c
• all mice die within hours of birth

nervous system
• as early as E11.5
• at E13.5, brain vasculature exhibits extensive cavitation compared to in control mice
• in newborn mice, brain vessels are enlarged and hyperplastic compared to in control mice
• as early as E11.5
• severe by E15.5
• in all mice
• associated with brain hemorrhage
• enlarged ventricles

cardiovascular system
• as early as E11.5
• at E13.5, brain vasculature exhibits extensive cavitation compared to in control mice
• in newborn mice, brain vessels are enlarged and hyperplastic compared to in control mice
• as early as E11.5
• severe by E15.5
• in all mice

craniofacial
• more extensive complete cleft of the secondary palate compared to in Tgfb3tm1Doe homozygotes on the same background

respiratory system
N
• mice exhibit normal lung morphology

digestive/alimentary system
• more extensive complete cleft of the secondary palate compared to in Tgfb3tm1Doe homozygotes on the same background

growth/size/body
• more extensive complete cleft of the secondary palate compared to in Tgfb3tm1Doe homozygotes on the same background




Genotype
MGI:5008265
cx10
Allelic
Composition
Tgfb2tm1Doe/Tgfb2+
Tgfb3tm1Doe/Tgfb3tm1Doe
Genetic
Background
involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfb2tm1Doe mutation (2 available); any Tgfb2 mutation (36 available)
Tgfb3tm1Doe mutation (1 available); any Tgfb3 mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
limbs/digits/tail
N
• mice exhibit normal apoptosis of interdigital webbing

vision/eye
N
• mice exhibit normal retinal morphology




Genotype
MGI:5008266
cx11
Allelic
Composition
Tgfb2tm1Doe/Tgfb2tm1Doe
Tgfb3tm1Doe/Tgfb3tm1Doe
Genetic
Background
involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfb2tm1Doe mutation (2 available); any Tgfb2 mutation (36 available)
Tgfb3tm1Doe mutation (1 available); any Tgfb3 mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• retinal cell death is reduced compared to in wild-type mice
• cornea appear to exfoliate unlike in wild-type mice
• mice exhibit vascularized accumulation of cells in the posterior chamber of the eye unlike in wild-type mice
• lens epithelium thickness is decreased compared to in wild-type mice
• mice exhibit thickened neural retina compared with wild-type mice
• the neural retina is consistently detached from the pigment epithelium unlike in wild-type mice
• however, the outer retina and optic fiber layers are of normal thickness

limbs/digits/tail
• at E15.5
• at E13.5 and E14.5, mice lack mesenchymal indentation between future digits unlike wild-type mice
• at E13.5 and E14.5, mice exhibit decreased apoptosis in the interdigital zone compared to in wild-type mice

skeleton
• at E15.5, chondrogenesis in the digits is accelerated compared to in wild-type mice
• large hypertrophied chondrocytes with huge nuclei

cellular
• at E13.5 and E14.5, mice exhibit decreased apoptosis in the interdigital zone compared to in wild-type mice
• retinal cell death is reduced compared to in wild-type mice




Genotype
MGI:5008267
cx12
Allelic
Composition
Tgfb2tm1Doe/Tgfb2tm1Doe
Tgfb3tm1Doe/Tgfb3+
Genetic
Background
involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfb2tm1Doe mutation (2 available); any Tgfb2 mutation (36 available)
Tgfb3tm1Doe mutation (1 available); any Tgfb3 mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• retinal cell death is reduced compared to in wild-type mice
• with detached areas
• mice exhibit vascularized accumulation of cells in the posterior chamber of the eye unlike in wild-type mice
• lens epithelium thickness is decreased compared to in wild-type mice
• mice exhibit thickened neural retina compared with wild-type mice
• the neural retina is consistently detached from the pigment epithelium unlike in wild-type mice
• however, the outer retina and optic fiber layers are of normal thickness

limbs/digits/tail
• at E13.5, mice exhibit decreased apoptosis in the interdigital zone compared to in wild-type mice
• at E15.5

skeleton
• at E15.5, chondrogenesis in the digits is accelerated compared to in wild-type mice

cellular
• at E13.5, mice exhibit decreased apoptosis in the interdigital zone compared to in wild-type mice
• retinal cell death is reduced compared to in wild-type mice




Genotype
MGI:5008269
cx13
Allelic
Composition
Tgfb2tm1Doe/Tgfb2+
Tgfb3tm1Doe/Tgfb3+
Genetic
Background
involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfb2tm1Doe mutation (2 available); any Tgfb2 mutation (36 available)
Tgfb3tm1Doe mutation (1 available); any Tgfb3 mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• mice exhibit reduced enterocyte apoptosis in the small intestine compared with wild-type mice
• however, enterocyte apoptosis in the colon is normal
• villus length is increased compared to in wild-type mice

cellular
• mice exhibit reduced enterocyte apoptosis in the small intestine compared with wild-type mice
• however, enterocyte apoptosis in the colon is normal




Genotype
MGI:5008411
cx14
Allelic
Composition
Tgfb1tm1Jmu/Tgfb1tm1Jmu
Tgfb3tm1Doe/Tgfb3tm1Doe
Genetic
Background
involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6J * Swiss Webster
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfb1tm1Jmu mutation (0 available); any Tgfb1 mutation (35 available)
Tgfb3tm1Doe mutation (1 available); any Tgfb3 mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• very few mice are born
• Background Sensitivity: more mice are born than on a background lacking BALB/c, but fewer mice are born than on a background containing ICR

cardiovascular system
• in non-viable mice
• in all mice

embryo
• in non-viable mice

nervous system
• in all mice




Genotype
MGI:5008408
cx15
Allelic
Composition
Tgfb1tm1Jmu/Tgfb1tm1Jmu
Tgfb3tm1Doe/Tgfb3+
Genetic
Background
involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6J * Swiss Webster
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfb1tm1Jmu mutation (0 available); any Tgfb1 mutation (35 available)
Tgfb3tm1Doe mutation (1 available); any Tgfb3 mutation (35 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• most mice only survive 3 weeks before dying of widespread inflammation

cardiovascular system
• in non-viable mice

embryo
• in non-viable mice

nervous system





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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory