Phenotypes associated with this allele

• Allele Symbol: Jak3^tm1Ljb
• Allele Name: targeted mutation 1, Leslie J Berg
• Allele ID: MGI:1857276
• Summary: 9 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Jak3^tm1Ljb/Jak3^tm1Ljb	B6;129S4-Jak3^tm1Ljb/J	MGI:3769351
hm2	Jak3^tm1Ljb/Jak3^tm1Ljb	involves: 129S4/SvJae	MGI:3769345
hm3	Jak3^tm1Ljb/Jak3^tm1Ljb	involves: 129S4/SvJae * C57BL/6	MGI:2181271
ht4	Jak3^tm1Ljb/Jak3^+	involves: 129S4/SvJae	MGI:3769346
ht5	Jak3^M1Pgrs/Jak3^tm1Ljb	involves: 129S4/SvJae * C57BL/6J * C57BL/10J	MGI:5468017
cx6	Jak3^tm1Ljb/Jak3^tm1Ljb Rag1^tm1Mom/Rag1^tm1Mom	involves: 129S4/SvJae * 129S7/SvEvBrd	MGI:3769348
cx7	Jak3^tm1Ljb/Jak3^tm1Ljb Tg(Lck-Jak3)2Ljb/0	involves: 129S4/SvJae * C3H * C57BL/6	MGI:3769347
cx8	Jak3^tm1Ljb/Jak3^tm1Ljb Tg(Lck-Jak3)1Ljb/0	involves: 129S4/SvJae * C3H * C57BL/6 * C57BL/10	MGI:3769355
cx9	Jak3^tm1Ljb/Jak3^tm1Ljb Tg(Lck-Jak3)2Ljb/0	involves: 129S4/SvJae * C3H * C57BL/6 * C57BL/10	MGI:3769356

Genotype
MGI:3769351

hm1

• Allelic Composition: Jak3^tm1Ljb/Jak3^tm1Ljb
• Genetic Background: B6;129S4-Jak3^tm1Ljb/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased CD4-positive, alpha-beta T cell number ( J:123902 )

• decrease in the proportion and absolute number of CD4+ cells in the spleen

decreased CD8-positive, alpha-beta T cell number ( J:123902 )

• decrease in the proportion and absolute number of CD8+ cells in the spleen

abnormal dendritic cell morphology ( J:123902 )

abnormal dendritic cell differentiation ( J:123902 )

• splenic dendritic cells express higher levels of activation markers compared to cells from wild-type controls

• however, bone marrow precursors in vitro show a similar capacity to generate immature dendritic cells compared to wild-type controls

decreased myeloid dendritic cell number ( J:123902 )

• the proportion and absolute number of CD11c+ dendritic cells is decreased in the spleen

• however, the frequency plasmacytoid dendritic cells in the spleen is not significantly different from wild-type controls

abnormal splenic cell ratio ( J:123902 )

• reduced numbers of splenocytes

• decrease in the proportion and absolute number of CD11c+ dendritic, CD4+, and CD8+ cells in the spleen

• however, the frequency plasmacytoid dendritic cells is not significantly different from wild-type controls

abnormal dendritic cell physiology ( J:123902 )

• in culture, dendritic cells are resistant to cytokine withdrawal-induced apoptosis

• bone marrow-derived dendritic cells overproduce IL12 and IL10

• when bone marrow-derived dendritic cells are used in an adoptive transfer model the proportion of IFNG-producing cells is increased compared to when wild-type cells are used

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:123902 )

• decrease in the proportion and absolute number of CD4+ cells in the spleen

decreased CD8-positive, alpha-beta T cell number ( J:123902 )

• decrease in the proportion and absolute number of CD8+ cells in the spleen

abnormal dendritic cell morphology ( J:123902 )

abnormal dendritic cell differentiation ( J:123902 )

• splenic dendritic cells express higher levels of activation markers compared to cells from wild-type controls

• however, bone marrow precursors in vitro show a similar capacity to generate immature dendritic cells compared to wild-type controls

decreased myeloid dendritic cell number ( J:123902 )

• the proportion and absolute number of CD11c+ dendritic cells is decreased in the spleen

• however, the frequency plasmacytoid dendritic cells in the spleen is not significantly different from wild-type controls

abnormal splenic cell ratio ( J:123902 )

• reduced numbers of splenocytes

• decrease in the proportion and absolute number of CD11c+ dendritic, CD4+, and CD8+ cells in the spleen

• however, the frequency plasmacytoid dendritic cells is not significantly different from wild-type controls

cellular

abnormal dendritic cell differentiation ( J:123902 )

• splenic dendritic cells express higher levels of activation markers compared to cells from wild-type controls

• however, bone marrow precursors in vitro show a similar capacity to generate immature dendritic cells compared to wild-type controls

Genotype
MGI:3769345

hm2

• Allelic Composition: Jak3^tm1Ljb/Jak3^tm1Ljb
• Genetic Background: involves: 129S4/SvJae

immune system

abnormal thymus cell ratio ( J:128694 )

• increase in the ratio of CD4+ to CD8+ cells

thymus hypoplasia ( J:128694 )

abnormal myelopoiesis ( J:56629 )

• increase in the number of myeloid progenitor cells in the peripheral blood and bone marrow

abnormal leukocyte morphology ( J:56629 )

• large increase in the number of cells classified as myeloid or premonocytic

abnormal T cell differentiation ( J:64861 )

• the proportion of pro-T cells is increased about 3-fold and fewer cells in transition between pre-T and late pre-T cell stages are found

• both pro-T and pre-T cells have increased CD25 expression

• about a 2-fold increase in apoptotic thymocytes is seen from E14 - E17

• intrathymically injected bone marrow cells show extremely limited ability to reconstitute T cell development especially when in competition with heterozygous or wild-type cells

• most intrathymically injected cells arrest at the CD44+ CD25- double negativeprogenitor cell stage

• from E15 - E17, T cell maturation appears to be delayed by about 1 day compared to age-matched heterozygous littermates

• however, by E18 T cell maturation in homozygous mice has caught up to that of their heterozygous littermates

decreased lymphocyte cell number ( J:56629 )

• lymphopenia is seen in peripheral and bone marrow smears

• at 6-12 months of age, lymphocyte numbers in whole blood suspensions are decreased

decreased thymocyte number ( J:64861 )

• in adult mice, there is about a 20-fold reduction in thymocyte numbers

• at E14 - E15, thymocytes are virtually undetectable

• at E14 only about 200 Thy+ cells are present in the thymus compared to around 20,000 in age-matched heterozygous littermates

• despite the deficit in progenitors, the rate of thymocyte expansion between E14 and E18 and differentiation of thymic progenitors into pro-T cells are similar to that of heterozygous littermates

absent B cells ( J:128694 )

increased leukocyte cell number ( J:56629 )

• at 6-12 months of age, a profound increase in large granular cells is seen in the peripheral blood and spleen

• these large granular cells consist of 2 populations; cells of the neutrophilic lineage and cells of the monocytic lineage

increased neutrophil cell number ( J:56629 )

• significantly higher numbers of both segmented and band neutrophils

increased monocyte cell number ( J:56629 )

• at 6 - 12 months of age in the bone marrow and spleen

abnormal spleen morphology ( J:56629 )

• the general architecture of the spleen is severely disrupted

enlarged spleen ( J:56629 )

• may be seen in some mice by as early as 4 months of age

• seen in all mice over 5 months of age

abnormal spleen white pulp morphology ( J:56629 )

• the white pulp is replaced with a collection of large cells with euchromatic nuclei that are CD3+

• numerous megakaryoctes are present

abnormal T cell physiology ( J:128694 )

• thymocytes produce less IL12 and IL13 in response to anti-CD3 plus anti-CD28-stimulation compared to wild-type controls

• splenic T cells secrete less IL12 in response to T cell receptor plus CD28 stimulation

abnormal T cell activation ( J:56629 , J:128694 )

• CD4+ cells that are present show an activated phenotype (J:56629)

• CD4+ T cells resemble activated or memory T cells (J:128694)

decreased T cell proliferation ( J:128694 )

• decrease in thymocyte proliferation in response to CD3 plus CD28 stimulation compared to wild-type

• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is virtually absent

absent lymph nodes ( J:128694 )

chronic inflammation ( J:56629 )

• widespread infiltration of the lungs, kidneys, and liver with mononuclear cells

hematopoietic system

abnormal thymus cell ratio ( J:128694 )

• increase in the ratio of CD4+ to CD8+ cells

thymus hypoplasia ( J:128694 )

abnormal erythropoiesis ( J:56629 )

• slight decrease in the number of erythroid progenitors in the bone marrow

abnormal myelopoiesis ( J:56629 )

• increase in the number of myeloid progenitor cells in the peripheral blood and bone marrow

abnormal leukocyte morphology ( J:56629 )

• large increase in the number of cells classified as myeloid or premonocytic

abnormal T cell differentiation ( J:64861 )

• the proportion of pro-T cells is increased about 3-fold and fewer cells in transition between pre-T and late pre-T cell stages are found

• both pro-T and pre-T cells have increased CD25 expression

• about a 2-fold increase in apoptotic thymocytes is seen from E14 - E17

• intrathymically injected bone marrow cells show extremely limited ability to reconstitute T cell development especially when in competition with heterozygous or wild-type cells

• most intrathymically injected cells arrest at the CD44+ CD25- double negativeprogenitor cell stage

• from E15 - E17, T cell maturation appears to be delayed by about 1 day compared to age-matched heterozygous littermates

• however, by E18 T cell maturation in homozygous mice has caught up to that of their heterozygous littermates

decreased lymphocyte cell number ( J:56629 )

• lymphopenia is seen in peripheral and bone marrow smears

• at 6-12 months of age, lymphocyte numbers in whole blood suspensions are decreased

decreased thymocyte number ( J:64861 )

• in adult mice, there is about a 20-fold reduction in thymocyte numbers

• at E14 - E15, thymocytes are virtually undetectable

• at E14 only about 200 Thy+ cells are present in the thymus compared to around 20,000 in age-matched heterozygous littermates

• despite the deficit in progenitors, the rate of thymocyte expansion between E14 and E18 and differentiation of thymic progenitors into pro-T cells are similar to that of heterozygous littermates

absent B cells ( J:128694 )

increased leukocyte cell number ( J:56629 )

• at 6-12 months of age, a profound increase in large granular cells is seen in the peripheral blood and spleen

• these large granular cells consist of 2 populations; cells of the neutrophilic lineage and cells of the monocytic lineage

increased neutrophil cell number ( J:56629 )

• significantly higher numbers of both segmented and band neutrophils

increased monocyte cell number ( J:56629 )

• at 6 - 12 months of age in the bone marrow and spleen

abnormal spleen morphology ( J:56629 )

• the general architecture of the spleen is severely disrupted

enlarged spleen ( J:56629 )

• may be seen in some mice by as early as 4 months of age

• seen in all mice over 5 months of age

abnormal spleen white pulp morphology ( J:56629 )

• the white pulp is replaced with a collection of large cells with euchromatic nuclei that are CD3+

• numerous megakaryoctes are present

abnormal T cell physiology ( J:128694 )

• thymocytes produce less IL12 and IL13 in response to anti-CD3 plus anti-CD28-stimulation compared to wild-type controls

• splenic T cells secrete less IL12 in response to T cell receptor plus CD28 stimulation

abnormal T cell activation ( J:56629 , J:128694 )

• CD4+ cells that are present show an activated phenotype (J:56629)

• CD4+ T cells resemble activated or memory T cells (J:128694)

decreased T cell proliferation ( J:128694 )

• decrease in thymocyte proliferation in response to CD3 plus CD28 stimulation compared to wild-type

• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is virtually absent

endocrine/exocrine glands

abnormal thymus cell ratio ( J:128694 )

• increase in the ratio of CD4+ to CD8+ cells

thymus hypoplasia ( J:128694 )

decreased thymocyte number ( J:64861 )

• in adult mice, there is about a 20-fold reduction in thymocyte numbers

• at E14 - E15, thymocytes are virtually undetectable

• at E14 only about 200 Thy+ cells are present in the thymus compared to around 20,000 in age-matched heterozygous littermates

• despite the deficit in progenitors, the rate of thymocyte expansion between E14 and E18 and differentiation of thymic progenitors into pro-T cells are similar to that of heterozygous littermates

cellular

decreased T cell proliferation ( J:128694 )

• decrease in thymocyte proliferation in response to CD3 plus CD28 stimulation compared to wild-type

• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is virtually absent

growth/size/body

enlarged spleen ( J:56629 )

• may be seen in some mice by as early as 4 months of age

• seen in all mice over 5 months of age

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-positive, Nk cell-positive		DOID:0090014	OMIM:608971	J:64861

Genotype
MGI:2181271

hm3

• Allelic Composition: Jak3^tm1Ljb/Jak3^tm1Ljb
• Genetic Background: involves: 129S4/SvJae * C57BL/6

immune system

small thymus ( J:29722 )

thymus hypoplasia ( J:29722 )

• total cell numbers range from 0.5 to 10% of wild-type controls

• however, the proportion of different cell types is similar to controls

abnormal B cell morphology ( J:29722 )

decreased immature B cell number ( J:29722 )

• decreased numbers of CD43- CD45R+ cells are detected in the bone marrow or spleen

• however, no significant decrease in the number of CD43+ CD45R+ cells is detected

arrested B cell differentiation ( J:29722 )

• profound block in B cell development at the pre-B cell stage

• however, total bone marrow cell numbers are similar to wild-type controls

decreased mature B cell number ( J:29722 )

• virtually no CD45R+ IgM+ cells are detected in the bone marrow or spleen

• peritoneal lavage cells contain significantly fewer CD45R+ IgM+ cells

spleen hypoplasia ( J:29722 )

• total spleen cell numbers range from 10 to 500% of wild-type controls

abnormal splenic cell ratio ( J:29722 )

• spleens contain an increased percentage of Thy1- CD45R- Mac1- cells

small Peyer's patches ( J:29722 )

• Peyer's patches are almost undetectable

small lymph nodes ( J:29722 )

• peripheral lymph nodes are nearly undetectable

lymph node hypoplasia ( J:29722 )

• peripheral lymph nodes contain only about 2% the normal number of cells

• the ratio of CD4+ to CD8+ cells is increase with more than 60% of the cells being CD4+ and less than 5% being CD8+

abnormal immune system physiology ( J:29722 )

abnormal leukocyte physiology ( J:90510 )

• decrease in the chemotactic response of thymocytes towards CCL25 and CXCL12

• however, chemotactic responses towards CCL5, CCL11 and CCL14 are not significantly different from heterozygous controls

abnormal lymphocyte physiology ( J:90510 )

• decrease in the chemotactic response of bone marrow cells towards CCL25 and CXCL12

• cells that do migrate towards CCL25 and CXCL12 are enriched for hematopoietic progenitors

decreased IgG level ( J:29722 )

• serum IgG levels appear to be lower in mice at 3 months of age

• however, in younger mice serum Ig levels are similar to wild-type

abnormal T cell activation ( J:29722 )

• splenic T cells stimulated with anti-CD3 plus anti-CD28 secrete significantly less IL-2

• most of the splenic T cells express markers of activation and are larger than wild-type splenic T cells

decreased T cell proliferation ( J:29722 )

• mitogen-induced thymocyte and splenic T cell proliferation responses are significantly reduced compared to wild-type cells

abnormal response to infection ( J:29722 )

• the LPS-induced proliferation response of spleen cells is significantly reduced

hematopoietic system

small thymus ( J:29722 )

thymus hypoplasia ( J:29722 )

• total cell numbers range from 0.5 to 10% of wild-type controls

• however, the proportion of different cell types is similar to controls

abnormal B cell morphology ( J:29722 )

decreased immature B cell number ( J:29722 )

• decreased numbers of CD43- CD45R+ cells are detected in the bone marrow or spleen

• however, no significant decrease in the number of CD43+ CD45R+ cells is detected

arrested B cell differentiation ( J:29722 )

• profound block in B cell development at the pre-B cell stage

• however, total bone marrow cell numbers are similar to wild-type controls

decreased mature B cell number ( J:29722 )

• virtually no CD45R+ IgM+ cells are detected in the bone marrow or spleen

• peritoneal lavage cells contain significantly fewer CD45R+ IgM+ cells

spleen hypoplasia ( J:29722 )

• total spleen cell numbers range from 10 to 500% of wild-type controls

abnormal splenic cell ratio ( J:29722 )

• spleens contain an increased percentage of Thy1- CD45R- Mac1- cells

abnormal leukocyte physiology ( J:90510 )

• decrease in the chemotactic response of thymocytes towards CCL25 and CXCL12

• however, chemotactic responses towards CCL5, CCL11 and CCL14 are not significantly different from heterozygous controls

abnormal lymphocyte physiology ( J:90510 )

• decrease in the chemotactic response of bone marrow cells towards CCL25 and CXCL12

• cells that do migrate towards CCL25 and CXCL12 are enriched for hematopoietic progenitors

decreased IgG level ( J:29722 )

• serum IgG levels appear to be lower in mice at 3 months of age

• however, in younger mice serum Ig levels are similar to wild-type

abnormal T cell activation ( J:29722 )

• splenic T cells stimulated with anti-CD3 plus anti-CD28 secrete significantly less IL-2

• most of the splenic T cells express markers of activation and are larger than wild-type splenic T cells

decreased T cell proliferation ( J:29722 )

• mitogen-induced thymocyte and splenic T cell proliferation responses are significantly reduced compared to wild-type cells

endocrine/exocrine glands

small thymus ( J:29722 )

thymus hypoplasia ( J:29722 )

• total cell numbers range from 0.5 to 10% of wild-type controls

• however, the proportion of different cell types is similar to controls

cellular

decreased T cell proliferation ( J:29722 )

• mitogen-induced thymocyte and splenic T cell proliferation responses are significantly reduced compared to wild-type cells

Genotype
MGI:3769346

ht4

• Allelic Composition: Jak3^tm1Ljb/Jak3⁺
• Genetic Background: involves: 129S4/SvJae

immune system

abnormal leukocyte morphology ( J:56629 )

• slight increase in the number of cells classified as myeloid or premonocytic

decreased lymphocyte cell number ( J:56629 )

• lymphopenia is seen in peripheral and bone marrow smears

• at 6-12 months of age, lymphocyte numbers in whole blood suspensions are decreased

increased leukocyte cell number ( J:56629 )

• at 6-12 months of age, a profound increase in large granular cells is seen in the peripheral blood and spleen

• these large granular cells consist of 2 populations; cells of the neutrophilic lineage and cells of the monocytic lineage

increased neutrophil cell number ( J:56629 )

• significantly higher numbers of both segmented and band neutrophils

increased monocyte cell number ( J:56629 )

• at 6 - 12 months of age in the bone marrow and spleen

hematopoietic system

abnormal leukocyte morphology ( J:56629 )

• slight increase in the number of cells classified as myeloid or premonocytic

decreased lymphocyte cell number ( J:56629 )

• lymphopenia is seen in peripheral and bone marrow smears

• at 6-12 months of age, lymphocyte numbers in whole blood suspensions are decreased

increased leukocyte cell number ( J:56629 )

• at 6-12 months of age, a profound increase in large granular cells is seen in the peripheral blood and spleen

• these large granular cells consist of 2 populations; cells of the neutrophilic lineage and cells of the monocytic lineage

increased neutrophil cell number ( J:56629 )

• significantly higher numbers of both segmented and band neutrophils

increased monocyte cell number ( J:56629 )

• at 6 - 12 months of age in the bone marrow and spleen

Genotype
MGI:5468017

ht5

• Allelic Composition: Jak3^M1Pgrs/Jak3^tm1Ljb
• Genetic Background: involves: 129S4/SvJae * C57BL/6J * C57BL/10J

mortality/aging

decreased susceptibility to parasitic infection induced morbidity/mortality ( J:185217 )

• mice are resistant lethality associated with cerebral malaria induced by infection with Plasmodium berghei ANKA-parasitized red blood cells compared with C57BL/10J mice

immune system

decreased susceptibility to parasitic infection induced morbidity/mortality ( J:185217 )

• mice are resistant lethality associated with cerebral malaria induced by infection with Plasmodium berghei ANKA-parasitized red blood cells compared with C57BL/10J mice

Genotype
MGI:3769348

cx6

• Allelic Composition: Jak3^tm1Ljb/Jak3^tm1Ljb; Rag1^tm1Mom/Rag1^tm1Mom
• Genetic Background: involves: 129S4/SvJae * 129S7/SvEvBrd

immune system

immune system phenotype ( J:56629 )

N • unlike Jak3 null mice that are wild-type for Rag1, no splenomegaly is seen

abnormal splenic cell ratio ( J:56629 )

• FACs profile of spleens resemble those of mice homozygous null for Rag1 alone

hematopoietic system

abnormal splenic cell ratio ( J:56629 )

• FACs profile of spleens resemble those of mice homozygous null for Rag1 alone

Genotype
MGI:3769347

cx7

• Allelic Composition: Jak3^tm1Ljb/Jak3^tm1Ljb; Tg(Lck-Jak3)2Ljb/0
• Genetic Background: involves: 129S4/SvJae * C3H * C57BL/6

immune system

immune system phenotype ( J:56629 )

N • unlike in homozygous null mice that do not express the transgene, the numbers of CD4 and CD8 cells in the spleen and thymus are normal

enlarged spleen ( J:56629 )

• splenomegaly apparent after 12 weeks of age

• spleen size is 2 to 3 times that of age-matched homozygous null mice that do not express the transgene

increased leukocyte cell number ( J:56629 )

• an expansion of large granular cells is seen, similar to homozygous null mice that do not express the transgene

abnormal T cell activation ( J:56629 )

• CD4+ cells show an activated phenotype

hematopoietic system

enlarged spleen ( J:56629 )

• splenomegaly apparent after 12 weeks of age

• spleen size is 2 to 3 times that of age-matched homozygous null mice that do not express the transgene

increased leukocyte cell number ( J:56629 )

• an expansion of large granular cells is seen, similar to homozygous null mice that do not express the transgene

abnormal T cell activation ( J:56629 )

• CD4+ cells show an activated phenotype

growth/size/body

enlarged spleen ( J:56629 )

• splenomegaly apparent after 12 weeks of age

• spleen size is 2 to 3 times that of age-matched homozygous null mice that do not express the transgene

Genotype
MGI:3769355

cx8

• Allelic Composition: Jak3^tm1Ljb/Jak3^tm1Ljb; Tg(Lck-Jak3)1Ljb/0
• Genetic Background: involves: 129S4/SvJae * C3H * C57BL/6 * C57BL/10

immune system

immune system phenotype ( J:128694 )

N • unlike null mice without the transgene, thymic cellularity, the ratio of CD4+ to CD8+ cells in the thymus, T cell maturation in the spleen, and the numbers of gamma delta T and NK cells are normal

decreased T cell proliferation ( J:128694 )

• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is reduced

absent B cells ( J:128694 )

• absence of CD45R+ IgM+ cells in the bone marrow

absent lymph nodes ( J:128694 )

hematopoietic system

decreased T cell proliferation ( J:128694 )

• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is reduced

absent B cells ( J:128694 )

• absence of CD45R+ IgM+ cells in the bone marrow

cellular

decreased T cell proliferation ( J:128694 )

• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is reduced

Genotype
MGI:3769356

cx9

• Allelic Composition: Jak3^tm1Ljb/Jak3^tm1Ljb; Tg(Lck-Jak3)2Ljb/0
• Genetic Background: involves: 129S4/SvJae * C3H * C57BL/6 * C57BL/10

immune system

immune system phenotype ( J:128694 )

N • unlike null mice without the transgene, thymic cellularity, the ratio of CD4+ to CD8+ cells in the thymus, T cell maturation in the spleen, and the numbers of gamma delta T and NK cells are normal

absent B cells ( J:128694 )

• absence of CD45R+ IgM+ cells in the bone marrow

abnormal T cell physiology ( J:128694 )

• the ability of splenic T cells to secrete IL12 in response to T cell receptor plus CD28 stimulation decreases with age (from 25 to 42 days of age)

abnormal T cell activation ( J:128694 )

• as mice age the proportion of CD4+ T cells that resemble activated or memory T cells increases

• this correlates with the decrease in transgene expression in the periphery as mice age

decreased T cell proliferation ( J:128694 )

• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is reduced

absent lymph nodes ( J:128694 )

hematopoietic system

absent B cells ( J:128694 )

• absence of CD45R+ IgM+ cells in the bone marrow

abnormal T cell physiology ( J:128694 )

• the ability of splenic T cells to secrete IL12 in response to T cell receptor plus CD28 stimulation decreases with age (from 25 to 42 days of age)

abnormal T cell activation ( J:128694 )

• as mice age the proportion of CD4+ T cells that resemble activated or memory T cells increases

• this correlates with the decrease in transgene expression in the periphery as mice age

decreased T cell proliferation ( J:128694 )

• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is reduced

cellular

decreased T cell proliferation ( J:128694 )

• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is reduced