Phenotypes associated with this allele

• Allele Symbol: Rasa1^tm1Paw
• Allele Name: targeted mutation 1, Tony Pawson
• Allele ID: MGI:1857417
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rasa1^tm1Paw/Rasa1^tm1Paw	either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * CD-1)	MGI:3040174
cx2	Rasa1^tm1Paw/Rasa1^tm1Paw Tek^tm1Dmt/Tek^tm1Dmt	either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * CD-1)	MGI:3040175
cx3	Nf1^tm1Tyj/Nf1^tm1Tyj Rasa1^tm1Paw/Rasa1^tm1Paw	either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * CD-1)	MGI:3040176

Genotype
MGI:3040174

hm1

• Allelic Composition: Rasa1^tm1Paw/Rasa1^tm1Paw
• Genetic Background: either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * CD-1)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:29825 )

• at E8.5 when embryo turning occurs, embryos were normal

• embryos were visibly abnormal by E9.25 and died by E10.5

cellular

abnormal cell death ( J:29825 )

• localized regions of cell death by E9.0 in the first branchial arch, hindbrain, optic stalk and telencephalon

embryo

abnormal developmental patterning ( J:29825 )

abnormal rostral-caudal axis patterning ( J:29825 )

• defect in posterior elongation by E9.25

embryonic growth arrest ( J:29825 )

• development is arrested by E9.5

decreased embryo size ( J:29825 )

• by E9.5, embryos were significantly smaller than normal littermates

abnormal embryonic tissue morphology ( J:29825 )

abnormal first pharyngeal arch morphology ( J:29825 )

• apoptotic cells in the neural crest of the first branchial arch

incomplete somite formation ( J:29825 )

• fewer than 25 somites at E9.5

excessive folding of visceral yolk sac ( J:29825 )

• yolk sac had an abnormal roughened or wrinkled appearance by E9.5

growth/size/body

decreased embryo size ( J:29825 )

• by E9.5, embryos were significantly smaller than normal littermates

craniofacial

abnormal first pharyngeal arch morphology ( J:29825 )

• apoptotic cells in the neural crest of the first branchial arch

Genotype
MGI:3040175

cx2

• Allelic Composition: Rasa1^tm1Paw/Rasa1^tm1Paw; Tek^tm1Dmt/Tek^tm1Dmt
• Genetic Background: either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * CD-1)

cardiovascular system

abnormal dorsal aorta morphology ( J:29825 )

• at 16 somite stage, thinning of the dorsal aorta and aberrant ventral arteries seen

absent vitelline blood vessels ( J:29825 )

distended pericardium ( J:29825 )

• heart is surrounded by a distended pericardium by E9.5

hemorrhage ( J:29825 )

• by E9.5, local ruptures in the vasculature and leakage of blood into the body cavity occurred

embryo

absent vitelline blood vessels ( J:29825 )

abnormal visceral yolk sac morphology ( J:29825 )

abnormal vitelline vascular remodeling ( J:29825 )

• endothelial cells failed to reorganize into a vascular network

Genotype
MGI:3040176

cx3

• Allelic Composition: Nf1^tm1Tyj/Nf1^tm1Tyj; Rasa1^tm1Paw/Rasa1^tm1Paw
• Genetic Background: either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * CD-1)

cardiovascular system

abnormal heart morphology ( J:29825 )

• the heart and surrounding trunk are abnormal

embryo

increased cranial neural crest cell apoptosis ( J:29825 )

• cell death is widespread in migrating cranial neural crest

abnormal embryo turning ( J:29825 )

• embryo turning usually failed

embryonic growth arrest ( J:29825 )

• arrested development around E8.5

abnormal embryonic tissue morphology ( J:29825 )

abnormal pharyngeal arch morphology ( J:29825 )

• lacking or abnormal

absent limb buds ( J:29825 )

abnormal neural tube morphology ( J:29825 )

• localized regions of cell proliferation and outgrowth in the neural tube

failure of somite differentiation ( J:29825 )

• fewer than 8 disorganized somites

abnormal extraembryonic tissue morphology ( J:29825 )

enlarged allantois ( J:29825 )

excessive folding of visceral yolk sac ( J:29825 )

• ruffled yolk sac

hearing/vestibular/ear

absent otic vesicle ( J:29825 )

• otic placodes develop but do not develop into vesicles

nervous system

increased hindbrain apoptosis ( J:29825 )

• cell death was widespread in the hindbrain

abnormal neural tube morphology ( J:29825 )

• localized regions of cell proliferation and outgrowth in the neural tube

abnormal brain morphology ( J:29825 )

absent midbrain ( J:29825 )

• midbrain fails to develop

absent forebrain ( J:29825 )

• failure of forebrain structures to develop

abnormal hindbrain morphology ( J:29825 )

• dorsal surface of the hindbrain failed to close

craniofacial

abnormal pharyngeal arch morphology ( J:29825 )

• lacking or abnormal

limbs/digits/tail

absent limb buds ( J:29825 )

cellular

increased cranial neural crest cell apoptosis ( J:29825 )

• cell death is widespread in migrating cranial neural crest

increased hindbrain apoptosis ( J:29825 )

• cell death was widespread in the hindbrain