All Phenotypes E2f1<tm1Meg> MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

E2f1^tm1Meg
targeted mutation 1, Michael E Greenburg
MGI:1857424

Summary

20 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	E2f1^tm1Meg/E2f1^tm1Meg	either: (involves: 129/Sv * 129S4/SvJae) or (involves: 129/Sv * 129S4/SvJae * C57BL/6)	MGI:3621771
hm2	E2f1^tm1Meg/E2f1^tm1Meg	involves: 129 * C57BL/6 * FVB/N * NMRI	MGI:3722919
hm3	E2f1^tm1Meg/E2f1^tm1Meg	involves: 129S4/SvJae * C57BL/6	MGI:3722933
hm4	E2f1^tm1Meg/E2f1^tm1Meg	NOD.Cg-E2f1^tm1Meg	MGI:3621814
cn5	E2f1^tm1Meg/E2f1^tm1Meg E2f3^tm1.1Gle/E2f3^tm1.1Gle Rbl1^tm1Htr/Rbl1^tm1Htr Tg(Pax6-cre,GFP)2Pgr/?	involves: 129 * C57BL/6 * FVB/N * NMRI	MGI:3722927
cn6	E2f1^tm1Meg/E2f1^tm1Meg Rbl1^tm1Htr/Rbl1^tm1Htr Tg(Pax6-cre,GFP)2Pgr/?	involves: 129 * C57BL/6 * FVB/N * NMRI	MGI:3722918
cn7	E2f1^tm1Meg/E2f1⁺ Rbl1^tm1Htr/Rbl1^tm1Htr Tg(Pax6-cre,GFP)2Pgr/?	involves: 129 * C57BL/6 * FVB/N * NMRI	MGI:3722917
cx8	E2f1^tm1Meg/E2f1^tm1Meg E2f2^tm1Zubi/E2f2^tm1Zubi E2f3^tm2.1Gle/E2f3^tm2.1Gle	involves: 129 * FVB/N * NIH Black Swiss	MGI:3806839
cx9	E2f1^tm1Meg/E2f1^tm1Meg E2f2^tm1Zubi/E2f2^tm1Zubi E2f3^tm3.1Gle/E2f3^tm3.1Gle	involves: 129 * FVB/N * NIH Black Swiss	MGI:3806840
cx10	E2f1^tm1Meg/E2f1^tm1Meg Rb1^tm1Tyj/Rb1^tm1Tyj Tg(Rb1)1Blg/0	involves: 129S2/SvPas * 129S4/SvJae	MGI:4420469
cx11	E2f1^tm1Meg/E2f1^tm1Meg E2f3^tm2.1Gle/E2f3^tm2.1Gle	involves: 129S4/SvJae * 129S6/SvEvTac * FVB/N * NIH Black Swiss	MGI:3806844
cx12	E2f1^tm1Meg/E2f1^tm1Meg E2f3^tm3.1Gle/E2f3^tm3.1Gle	involves: 129S4/SvJae * 129S6/SvEvTac * FVB/N * NIH Black Swiss	MGI:3806846
cx13	E2f1^tm1Meg/E2f1^tm1Meg E2f3^tm5(E2f1)Gle/E2f3^tm5(E2f1)Gle	involves: 129S4/SvJae * 129S6/SvEvTac * FVB/N * NIH Black Swiss	MGI:3806847
cx14	E2f1^tm1Meg/E2f1^tm1Meg E2f3^tm4Gle/E2f3^tm4Gle	involves: 129S4/SvJae * 129S6/SvEvTac * FVB/N * NIH Black Swiss	MGI:3806848
cx15	E2f1^tm1Meg/E2f1^tm1Meg Rb1^tm2.1Fad/Rb1^tm2.1Fad	involves: 129S4/SvJae * C57BL/6	MGI:5614090
cx16	E2f1^tm1Meg/E2f1^tm1Meg Phactr4^humdy/Phactr4^humdy	involves: 129S4/SvJae * C57BL/6J	MGI:3721227
cx17	E2f1^tm1Meg/E2f1⁺ Phactr4^humdy/Phactr4^humdy	involves: 129S4/SvJae * C57BL/6J	MGI:3721228
cx18	E2f1^tm1Meg/E2f1⁺ Tg(CAG-Tfb1m)AGsha/0	involves: 129S4/SvJae * C57BL/6J * SJL/J	MGI:5488619
cx19	E2f1^tm1Meg/E2f1^tm1Meg H2-Oa/Brd2^{Tg(otx2-lacZ)F5pImat}/H2-Oa/Brd2^{Tg(otx2-lacZ)F5pImat}	involves: 129S4/SvJae * CD-1	MGI:5467720
cx20	H2-Oa/Brd2^{Tg(otx2-lacZ)F5pImat}/H2-Oa/Brd2^{Tg(otx2-lacZ)F5pImat} E2f1^tm1Meg/E2f1^tm1Meg	involves: 129S4/SvJae * CD-1	MGI:5467721

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg
Genetic Background	either: (involves: 129/Sv * 129S4/SvJae) or (involves: 129/Sv * 129S4/SvJae * C57BL/6)

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:33100 )

• as null animals age, they die at a significantly higher rate than wild-type littermates

growth/size/body

decreased body weight ( J:33100 )

• nulls weigh 17% less than wild-type littermates for at least the first 8 weeks of life

cellular

decreased apoptosis ( J:33100 )

• thymocytes from null mice show increased vialbility in culture with 0% viability over 24 hours compared with 40% viability of wild-type thymocytes

immune system

enlarged thymus ( J:33100 )

• in 4 to 6 week old null animals, thymi were noticeably enlarged compared to wild-type

increased thymus weight ( J:33100 )

• the ratio of thymus:body weight is 25% higher in nulls relative to wild-type

increased thymocyte number ( J:33100 )

• there is a 55% increase in number of thymocytes per thymus in null mice 4-6 weeks old

increased T cell derived lymphoma incidence ( J:33100 )

• one 6-12 month old animal exhibited a lymphoblastic lymphoma

abnormal double-positive T cell morphology ( J:33100 )

• double positive cells from null mice display clear enhancement of survival in culture compared to wild-type double-positive cells; in vivo, thymocytes from nulls show significantly less CD3epsilon induced apoptosis compared to wild-type

increased CD4-positive, alpha-beta T cell number ( J:33100 )

• thymuses of 4-6 week old null mice contain more mature CD4+ cells than wild-type

increased CD8-positive, alpha-beta T cell number ( J:33100 )

• thymuses of 4-6 week old null mice contain more mature CD8+ cells than wild-type

reproductive system

testicular atrophy ( J:33100 )

• in 6-12 month old mice, males show moderate testicular atrophy, characterized by a reduction in testicular weight

hematopoietic system

enlarged thymus ( J:33100 )

• in 4 to 6 week old null animals, thymi were noticeably enlarged compared to wild-type

increased thymus weight ( J:33100 )

• the ratio of thymus:body weight is 25% higher in nulls relative to wild-type

increased thymocyte number ( J:33100 )

• there is a 55% increase in number of thymocytes per thymus in null mice 4-6 weeks old

increased T cell derived lymphoma incidence ( J:33100 )

• one 6-12 month old animal exhibited a lymphoblastic lymphoma

abnormal double-positive T cell morphology ( J:33100 )

increased CD4-positive, alpha-beta T cell number ( J:33100 )

• thymuses of 4-6 week old null mice contain more mature CD4+ cells than wild-type

increased CD8-positive, alpha-beta T cell number ( J:33100 )

• thymuses of 4-6 week old null mice contain more mature CD8+ cells than wild-type

endocrine/exocrine glands

decreased salivation ( J:93708 )

• E2f1 knockouts have reduced salivary flow rates than NOD controls or the standard (NOD x B10.D2) F1 mice

enlarged thymus ( J:33100 )

• in 4 to 6 week old null animals, thymi were noticeably enlarged compared to wild-type

increased thymus weight ( J:33100 )

• the ratio of thymus:body weight is 25% higher in nulls relative to wild-type

increased thymocyte number ( J:33100 )

• there is a 55% increase in number of thymocytes per thymus in null mice 4-6 weeks old

increased T cell derived lymphoma incidence ( J:33100 )

• one 6-12 month old animal exhibited a lymphoblastic lymphoma

testicular atrophy ( J:33100 )

• in 6-12 month old mice, males show moderate testicular atrophy, characterized by a reduction in testicular weight

neoplasm

increased T cell derived lymphoma incidence ( J:33100 )

• one 6-12 month old animal exhibited a lymphoblastic lymphoma

digestive/alimentary system

decreased salivation ( J:93708 )

• E2f1 knockouts have reduced salivary flow rates than NOD controls or the standard (NOD x B10.D2) F1 mice

hearing/vestibular/ear

hearing/vestibular/ear phenotype ( J:98518 )

N	• at E18.5, homozygotes display a normal morphology of inner ear sensory epithelia relative to wild-type mice

homeostasis/metabolism

decreased salivation ( J:93708 )

• E2f1 knockouts have reduced salivary flow rates than NOD controls or the standard (NOD x B10.D2) F1 mice

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg
Genetic Background	involves: 129 * C57BL/6 * FVB/N * NMRI

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

vision/eye

decreased retina ganglion cell number ( J:124204 )

• mice have slightly fewer ganglion cells at P0

abnormal retina bipolar cell morphology ( J:124204 )

• mice have slightly fewer bipolar cells at P18 or P30

• however, the proportion of bipolar cells is normal

abnormal retina progenitor cell morphology ( J:124204 )

• retinal progenitor cell proliferation is decreased 2-fold

thin retina outer nuclear layer ( J:124204 )

• the retinal outer nuclear layer is slightly reduced in thickness at P18 or P30

abnormal cone electrophysiology ( J:124204 )

• photopic response is slightly reduced relative to normal

nervous system

decreased retina ganglion cell number ( J:124204 )

• mice have slightly fewer ganglion cells at P0

abnormal retina bipolar cell morphology ( J:124204 )

• mice have slightly fewer bipolar cells at P18 or P30

• however, the proportion of bipolar cells is normal

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg
Genetic Background	involves: 129S4/SvJae * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cellular

abnormal cell death ( J:75726 )

• cell death induced by Myc expression in primary fibroblast cells is severely impaired

• however, fibroblast cells retain their capacity to respond to other apoptotic signals

decreased T cell proliferation ( J:76318 )

• T cell receptor stimulated proliferation is impaired

immune system

decreased T cell proliferation ( J:76318 )

• T cell receptor stimulated proliferation is impaired

hematopoietic system

decreased T cell proliferation ( J:76318 )

• T cell receptor stimulated proliferation is impaired

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg
Genetic Background	NOD.Cg-E2f1^tm1Meg

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cellular

abnormal cell cycle ( J:93708 )

• fractions of cells in S phase from thymus and spleen of 5 week old mutant NOD mice are increased compared with wild-type, fractions of cells in G0-G1 in mutant thymus and spleen are reduced; in the spleen of mutants, number of cells in G2 and M phase are increased relative to wild-type

immune system

salivary gland inflammation ( J:93708 )

• large numbers of mononuclear inflammatory cells have infiltrated the salivary gland of 12-16 week old mutant NOD mice

enlarged thymus ( J:93708 )

• thymuses of 5 week old mutant NOD mice are enlarged with respect to wild-type NOD mice with a 55% increase in the number of thymocytes per thymus

insulitis ( J:93708 )

• large numbers of mononuclear inflammatory cells have infiltrated the salivary gland of 12-16 week old mutant NOD mice

abnormal double-positive T cell morphology ( J:93708 )

• null animals have lower numbers of double-positive Tcells than wild-type NOD animals

decreased double-positive T cell number ( J:93708 )

• null animals have lower numbers of double-positive Tcells than wild-type NOD animals

decreased regulatory T cell number ( J:93708 )

• numbers of CD4+CD25+ immunoregulatory T cells in the spleen of mutant NOD mice are decreased compared to wild-type NOD mice

increased T cell number ( J:93708 )

• mutant NOD mice have a greater absolute number of speen T cells

increased thymocyte number ( J:93708 )

• total numbers of thymocytes per thymus and absolute number of cells in all thymocyte populations are significantly increased in null NOD mice compared to wild-type NOD mice

abnormal CD4-positive, alpha beta T cell morphology ( J:93708 )

• thymuses of 5 week old null NOD mice contain more mature CD4+ cells than wild-type NOD animals

abnormal CD8-positive, alpha beta T cell morphology ( J:93708 )

• thymuses of 5 week old null NOD mice contain more mature CD8+ cells than wild-type NOD animals

increased interferon-gamma secretion ( J:93708 )

• spleen cells from null NOD mice stimulated with anti-CD3 produce increased levels of IFN gamma

increased interleukin-4 secretion ( J:93708 )

• production of IL-4 by spleen cells from null NOD mice is greater than wild-type NOD cells

increased susceptibility to autoimmune diabetes ( J:93708 )

• incidence of diabetes in knockout female mice is 88% and 58% in male mice, compared with 71% of female wild-type and 25% of male wild-type NOD mice by 32 weeks of age; time of onset is earlier and severity of diabetes is greater in knockouts than wild type littermates

• splenocytes from young mutant NOD mice can induce lympocytic infiltration of the salivary glands and pancreas and induce development of diabetes by spleen T cells from mutant NOD mice transferred into NOD.SCID recipients

endocrine/exocrine glands

decreased salivation ( J:93708 )

• knockout mice on the NOD background have reduced salivary flow rates than NOD or E2f1 knockout controls or the standard (NOD x B10.D2) F1 mice

salivary gland inflammation ( J:93708 )

• large numbers of mononuclear inflammatory cells have infiltrated the salivary gland of 12-16 week old mutant NOD mice

abnormal pancreatic islet morphology ( J:93708 )

• mononuclear inflammatory cells have infiltrated the pancreas islets of 12-16 week old prediabetic mutant NOD mice; there is some evidence of acinar cell degeneration and abnormally large nuclei or nuclei doubled in number are bserved

enlarged thymus ( J:93708 )

• thymuses of 5 week old mutant NOD mice are enlarged with respect to wild-type NOD mice with a 55% increase in the number of thymocytes per thymus

increased thymocyte number ( J:93708 )

• total numbers of thymocytes per thymus and absolute number of cells in all thymocyte populations are significantly increased in null NOD mice compared to wild-type NOD mice

insulitis ( J:93708 )

• large numbers of mononuclear inflammatory cells have infiltrated the salivary gland of 12-16 week old mutant NOD mice

hematopoietic system

enlarged thymus ( J:93708 )

• thymuses of 5 week old mutant NOD mice are enlarged with respect to wild-type NOD mice with a 55% increase in the number of thymocytes per thymus

abnormal double-positive T cell morphology ( J:93708 )

• null animals have lower numbers of double-positive Tcells than wild-type NOD animals

decreased double-positive T cell number ( J:93708 )

• null animals have lower numbers of double-positive Tcells than wild-type NOD animals

decreased regulatory T cell number ( J:93708 )

• numbers of CD4+CD25+ immunoregulatory T cells in the spleen of mutant NOD mice are decreased compared to wild-type NOD mice

increased T cell number ( J:93708 )

• mutant NOD mice have a greater absolute number of speen T cells

increased thymocyte number ( J:93708 )

• total numbers of thymocytes per thymus and absolute number of cells in all thymocyte populations are significantly increased in null NOD mice compared to wild-type NOD mice

abnormal CD4-positive, alpha beta T cell morphology ( J:93708 )

• thymuses of 5 week old null NOD mice contain more mature CD4+ cells than wild-type NOD animals

abnormal CD8-positive, alpha beta T cell morphology ( J:93708 )

• thymuses of 5 week old null NOD mice contain more mature CD8+ cells than wild-type NOD animals

digestive/alimentary system

decreased salivation ( J:93708 )

• knockout mice on the NOD background have reduced salivary flow rates than NOD or E2f1 knockout controls or the standard (NOD x B10.D2) F1 mice

salivary gland inflammation ( J:93708 )

• large numbers of mononuclear inflammatory cells have infiltrated the salivary gland of 12-16 week old mutant NOD mice

homeostasis/metabolism

decreased salivation ( J:93708 )

• knockout mice on the NOD background have reduced salivary flow rates than NOD or E2f1 knockout controls or the standard (NOD x B10.D2) F1 mice

Mouse Models of Human Disease	DO ID	OMIM ID(s)	Ref(s)
Sjogren's syndrome	DOID:12894	OMIM:270150	J:93708
type 1 diabetes mellitus	DOID:9744	OMIM:222100	J:93708

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg E2f3^tm1.1Gle/E2f3^tm1.1Gle Rbl1^tm1Htr/Rbl1^tm1Htr Tg(Pax6-cre,GFP)2Pgr/?
Genetic Background	involves: 129 * C57BL/6 * FVB/N * NMRI

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)
E2f3^tm1.1Gle mutation (0 available); any E2f3 mutation (33 available)
Rbl1^tm1Htr mutation (0 available); any Rbl1 mutation (60 available)
Tg(Pax6-cre,GFP)2Pgr mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

vision/eye

vision/eye phenotype ( J:124204 )

N	• bipolar and ganglion cell death, starburst amacrine cell (SAC) differentiation, and SAC track disorder observed in Rbl1 null, Rbl1/E2f3 null or Rbl1/E2f1 null mice are rescued

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg Rbl1^tm1Htr/Rbl1^tm1Htr Tg(Pax6-cre,GFP)2Pgr/?
Genetic Background	involves: 129 * C57BL/6 * FVB/N * NMRI

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)
Rbl1^tm1Htr mutation (0 available); any Rbl1 mutation (60 available)
Tg(Pax6-cre,GFP)2Pgr mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

vision/eye

abnormal retina morphology ( J:124204 )

• Slc18a3 staining of mature starburst amacrine cells (SACs) is absent from the peripheral retina

• however, retinal transition cell division, rod cell numbers, retinal differentiation and rod function are normal

decreased retina ganglion cell number ( J:124204 )

• mice have slightly fewer ganglion cells at P0

abnormal amacrine cell morphology ( J:124204 )

• starburst amacrine cells (SACs) have defects in differentiation not associated with cell cycle or apoptosis

• only 1 Calb2+ SAC track is detectable instead of 3 normally detected in the inner plexiform layer

• Slc18a3 staining of mature SACs is absent from the peripheral retina

• 3.7% of Camk2a+ SAC express Chat and Slc18a3 compared to 60% in wild-type mice, 5.6% in Rbl1 null mice and 91% in Rbl1 and E2f3 null mice

abnormal retina bipolar cell morphology ( J:124204 )

• mice have slightly fewer bipolar cells at P18 or P30

• however, the proportion of bipolar cells is normal

abnormal retina inner plexiform layer morphology ( J:124204 )

• only 1 Calb2+ starburst amacrine cell track is detectable instead of 3 normally detected in the inner plexiform layer

thin retina outer nuclear layer ( J:124204 )

• the retinal outer nuclear layer is slightly reduced in thickness at P18 or P30

abnormal cone electrophysiology ( J:124204 )

• photopic response is very slightly reduced

nervous system

decreased retina ganglion cell number ( J:124204 )

• mice have slightly fewer ganglion cells at P0

abnormal amacrine cell morphology ( J:124204 )

• starburst amacrine cells (SACs) have defects in differentiation not associated with cell cycle or apoptosis

• only 1 Calb2+ SAC track is detectable instead of 3 normally detected in the inner plexiform layer

• Slc18a3 staining of mature SACs is absent from the peripheral retina

• 3.7% of Camk2a+ SAC express Chat and Slc18a3 compared to 60% in wild-type mice, 5.6% in Rbl1 null mice and 91% in Rbl1 and E2f3 null mice

abnormal retina bipolar cell morphology ( J:124204 )

• mice have slightly fewer bipolar cells at P18 or P30

• however, the proportion of bipolar cells is normal

Allelic Composition	E2f1^tm1Meg/E2f1⁺ Rbl1^tm1Htr/Rbl1^tm1Htr Tg(Pax6-cre,GFP)2Pgr/?
Genetic Background	involves: 129 * C57BL/6 * FVB/N * NMRI

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

vision/eye

abnormal eye development ( J:124204 )

• ectopic retinal transition cell division observed in Rbl1 null mice is partially suppressed

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg E2f2^tm1Zubi/E2f2^tm1Zubi E2f3^tm2.1Gle/E2f3^tm2.1Gle
Genetic Background	involves: 129 * FVB/N * NIH Black Swiss

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)
E2f2^tm1Zubi mutation (1 available); any E2f2 mutation (29 available)
E2f3^tm2.1Gle mutation (0 available); any E2f3 mutation (33 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

perinatal lethality ( J:138289 )

cellular

decreased cell proliferation ( J:138289 )

• in MEFs after 5 and 6 days in culture compared to littermate-derived controls

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg E2f2^tm1Zubi/E2f2^tm1Zubi E2f3^tm3.1Gle/E2f3^tm3.1Gle
Genetic Background	involves: 129 * FVB/N * NIH Black Swiss

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)
E2f2^tm1Zubi mutation (1 available); any E2f2 mutation (29 available)
E2f3^tm3.1Gle mutation (0 available); any E2f3 mutation (33 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:138289 )

N	• unlike triple mutant mice lacking expression of the E2f3a isoform, triple mutant mice lacking the E2f3b isoform survive to adulthood

cellular

decreased cell proliferation ( J:138289 )

• in MEFs after 5 and 6 days in culture compared to littermate-derived controls

growth/size/body

decreased body weight ( J:138289 )

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg Rb1^tm1Tyj/Rb1^tm1Tyj Tg(Rb1)1Blg/0
Genetic Background	involves: 129S2/SvPas * 129S4/SvJae

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)
Rb1^tm1Tyj mutation (5 available); any Rb1 mutation (111 available)
Tg(Rb1)1Blg mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

muscle

abnormal skeletal muscle fiber morphology ( J:65679 )

• skeletal muscle fibers are no different than in Rb1^tm1Tyj/Rb1^tm1Tyj Tg(Rb1)1Blg mice

abnormal muscle physiology ( J:65679 )

• at E18.5, skeletal muscle exhibit an increase in apoptosis compared with Rb1^tm1Tyj/Rb1⁺ Tg(Rb1)1Blg mice

behavior/neurological

hyporesponsive to tactile stimuli ( J:65679 )

hunched posture ( J:65679 )

cellular

polyploidy ( J:65679 )

• as in Rb1^tm1Tyj/Rb1^tm1Tyj Tg(Rb1)1Blg mice

abnormal cell cycle ( J:65679 )

• as in Rb1^tm1Tyj/Rb1^tm1Tyj Tg(Rb1)1Blg mice, myotubes are unable to exhibit the cell cycle and accumulate enlarged nuclei unlike wild-type myotubes

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg E2f3^tm2.1Gle/E2f3^tm2.1Gle
Genetic Background	involves: 129S4/SvJae * 129S6/SvEvTac * FVB/N * NIH Black Swiss

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)
E2f3^tm2.1Gle mutation (0 available); any E2f3 mutation (33 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

decreased survivor rate ( J:138289 )

• only a few mice survive beyond 1 month of age

premature death ( J:138289 )

• most die within the first month of life

adipose tissue

adipose tissue phenotype ( J:138289 )

N	• despite loss of white fat, brown fat is relatively unaffected

decreased white adipose tissue amount ( J:138289 )

• by 3 weeks of age all white adipose deposits are absent

behavior/neurological

behavior/neurological phenotype ( J:138289 )

N	• no defect in eating behavior is detected, despite the absence of white fat

digestive/alimentary system

digestive/alimentary phenotype ( J:138289 )

N	• no defect in fat absorption is detected, despite the absence of white fat

growth/size/body

decreased body size ( J:138289 )

• severely runted by 3 weeks of age

decreased body weight ( J:138289 )

homeostasis/metabolism

decreased circulating insulin-like growth factor I level ( J:138289 )

• by 21 days of age

decreased circulating leptin level ( J:138289 )

• by 21 days of age

decreased circulating growth hormone level ( J:138289 )

• by 21 days of age

decreased circulating triglyceride level ( J:138289 )

cellular

decreased cell proliferation ( J:138289 )

• by 3 weeks of age the proliferative index in most tissues is reduced

reproductive system

abnormal corpus luteum morphology ( J:138289 )

• lack a well-developed corpus luteum

ovary hypoplasia ( J:138289 )

testis hypoplasia ( J:138289 )

• severe

female infertility ( J:138289 )

• the few females that reach reproductive age are infertile

male infertility ( J:138289 )

• the few males that reach reproductive age are infertile

skeleton

abnormal epiphyseal plate morphology ( J:138289 )

• at P21, long bone growth plates are severely dysplastic with many cells having mega-nuclei

endocrine/exocrine glands

abnormal adrenal cortex morphology ( J:138289 )

• the outer cortex lacks a functional zona fasciculata

abnormal corpus luteum morphology ( J:138289 )

• lack a well-developed corpus luteum

ovary hypoplasia ( J:138289 )

testis hypoplasia ( J:138289 )

• severe

nervous system

abnormal brain size ( J:138289 )

• by 21 days of age there is a relative increase in the brain weight to body weight ratio

respiratory system

abnormal lung epithelium morphology ( J:138289 )

• decrease in the branching of the epithelium

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg E2f3^tm3.1Gle/E2f3^tm3.1Gle
Genetic Background	involves: 129S4/SvJae * 129S6/SvEvTac * FVB/N * NIH Black Swiss

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)
E2f3^tm3.1Gle mutation (0 available); any E2f3 mutation (33 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

decreased body weight ( J:138289 )

• young mice are slightly but significantly smaller

• but by 90 days of age no significant difference in weight is detected

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg E2f3^tm5(E2f1)Gle/E2f3^tm5(E2f1)Gle
Genetic Background	involves: 129S4/SvJae * 129S6/SvEvTac * FVB/N * NIH Black Swiss

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)
E2f3^tm5(E2f1)Gle mutation (0 available); any E2f3 mutation (33 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

normal phenotype

no abnormal phenotype detected ( J:138289 )

• body weight, white adipose tissue deposition, bone morphology and growth, adrenal gland morphology and function, lung morphology, and gonad morphology are all normal

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg E2f3^tm4Gle/E2f3^tm4Gle
Genetic Background	involves: 129S4/SvJae * 129S6/SvEvTac * FVB/N * NIH Black Swiss

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)
E2f3^tm4Gle mutation (0 available); any E2f3 mutation (33 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

normal phenotype

no abnormal phenotype detected ( J:138289 )

• body weight, white adipose tissue deposition, bone morphology and growth, adrenal gland morphology and function, lung morphology, and gonad morphology are all normal

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg Rb1^tm2.1Fad/Rb1^tm2.1Fad
Genetic Background	involves: 129S4/SvJae * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)
Rb1^tm2.1Fad mutation (1 available); any Rb1 mutation (111 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality, incomplete penetrance ( J:215358 )

• fewer than expected mice are present at P14

neoplasm

neoplasm ( J:215358 )

N	• mice are tumor free beyond 1.5 years of life

cellular

cellular phenotype ( J:215358 )

N	• serum-starved fibroblasts exhibit normal cell cycle

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg Phactr4^humdy/Phactr4^humdy
Genetic Background	involves: 129S4/SvJae * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)
Phactr4^humdy mutation (1 available); any Phactr4 mutation (61 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

exencephaly ( J:123924 )

• only 2 of 5 mice display partial exencephaly with recovery of normal proliferation and differentiation in the neural tube indicating a rescue of the Phactr4^humdy phenotype

vision/eye

vision/eye phenotype ( J:123924 )

N	• mice do not display coloboma

Allelic Composition	E2f1^tm1Meg/E2f1⁺ Phactr4^humdy/Phactr4^humdy
Genetic Background	involves: 129S4/SvJae * C57BL/6J

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

exencephaly ( J:123924 )

• only 6 of 13 mice display partial exencephaly indicating a rescue of the Phactr4^humdy phenotype

vision/eye

vision/eye phenotype ( J:123924 )

N	• mice do not display coloboma

Allelic Composition	E2f1^tm1Meg/E2f1⁺ Tg(CAG-Tfb1m)AGsha/0
Genetic Background	involves: 129S4/SvJae * C57BL/6J * SJL/J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)
Tg(CAG-Tfb1m)AGsha mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Allelic Composition	E2f1^tm1Meg/E2f1^tm1Meg H2-Oa/Brd2^{Tg(otx2-lacZ)F5pImat}/H2-Oa/Brd2^{Tg(otx2-lacZ)F5pImat}
Genetic Background	involves: 129S4/SvJae * CD-1

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f1^tm1Meg mutation (2 available); any E2f1 mutation (28 available)
H2-Oa/Brd2^{Tg(otx2-lacZ)F5pImat} mutation (0 available); any H2-Oa mutation (11 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

embryo phenotype ( J:188139 )

N	• mice exhibit normal neurulation and neuroepithelial development with rescue of cell death

nervous system

nervous system phenotype ( J:188139 )

N	• mice exhibit normal neurulation and neuroepithelial development with rescue of cell death

Allelic Composition	H2-Oa/Brd2^{Tg(otx2-lacZ)F5pImat}/H2-Oa/Brd2^{Tg(otx2-lacZ)F5pImat} E2f1^tm1Meg/E2f1^tm1Meg
Genetic Background	involves: 129S4/SvJae * CD-1

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

embryo phenotype ( J:188139 )

N	• mice exhibit normal neurulation and neuroepithelial development with rescue of cell death

nervous system

nervous system phenotype ( J:188139 )

N	• mice exhibit normal neurulation and neuroepithelial development with rescue of cell death

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