Phenotypes associated with this allele

• Allele Symbol: Alox15^tm1Fun
• Allele Name: targeted mutation 1, Colin D Funk
• Allele ID: MGI:1857428
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Alox15^tm1Fun/Alox15^tm1Fun	B6.129S2-Alox15^tm1Fun	MGI:5517581
hm2	Alox15^tm1Fun/Alox15^tm1Fun	B6.129S2-Alox15^tm1Fun/J	MGI:3703438
hm3	Alox15^tm1Fun/Alox15^tm1Fun	involves: 129/Sv * C57BL/6	MGI:2429456
cx4	Alox15^tm1Fun/Alox15^tm1Fun Ldlr^tm1Her/Ldlr^tm1Her	involves: 129S2/SvPas * 129S7/SvEvBrd * C57BL/6	MGI:4367211

Genotype
MGI:5517581

hm1

• Allelic Composition: Alox15^tm1Fun/Alox15^tm1Fun
• Genetic Background: B6.129S2-Alox15^tm1Fun

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:197334 )

N • in a model of IgE antigen-dependent passive cutaneous anaphylaxis, mice exhibit normal dye extravasation (a measure of edema)

Genotype
MGI:3703438

hm2

• Allelic Composition: Alox15^tm1Fun/Alox15^tm1Fun
• Genetic Background: B6.129S2-Alox15^tm1Fun/J

growth/size/body

increased spleen weight ( J:119405 )

• progressive splenomegaly is observed after 8 weeks of age

mortality/aging

premature death ( J:119405 )

• increase in death rate of homozygous mice as compared to controls

• 80% survival at 12 months of age

• severe anemia is most likely cause of death

hematopoietic system

increased spleen weight ( J:119405 )

• progressive splenomegaly is observed after 8 weeks of age

abnormal myelopoiesis ( J:119405 )

• increase in numbers of myeloid cells in bone marrow, spleen, blood and lymph nodes as compared to control

• cell cycle analysis indicates an increase in proliferative capacity of splenic myeloid cells and a decrease in the percentage of apoptotic cells

anemia ( J:119405 )

• presumed cause of premature death

abnormal bone marrow cell number ( J:119405 )

• increase in number of myeloid cells and progenitors, as well as megkaryocytes

abnormal myeloblast morphology/development ( J:119405 )

• at death, cells in bone marrow of homozygous mice consist of greater than 25% myeloblasts

increased leukocyte cell number ( J:119405 )

• blood leukocystosis observed in asymptomatic and moribund mice

increased basophil cell number ( J:119405 )

• basophilia observed in asymptomatic and moribund mice

abnormal spleen red pulp morphology ( J:119405 )

• disrupted compartmentalization of red pulp

abnormal spleen white pulp morphology ( J:119405 )

• disrupted compartmentalization of white pulp

intermingled spleen red and white pulp ( J:119405 )

• moribund mice exhibit complete loss of splenic compartmentalization

decreased spleen B cell follicle number ( J:119405 )

• decrease in number of follicles

immune system

increased spleen weight ( J:119405 )

• progressive splenomegaly is observed after 8 weeks of age

abnormal myelopoiesis ( J:119405 )

• increase in numbers of myeloid cells in bone marrow, spleen, blood and lymph nodes as compared to control

• cell cycle analysis indicates an increase in proliferative capacity of splenic myeloid cells and a decrease in the percentage of apoptotic cells

increased leukocyte cell number ( J:119405 )

• blood leukocystosis observed in asymptomatic and moribund mice

increased basophil cell number ( J:119405 )

• basophilia observed in asymptomatic and moribund mice

abnormal spleen red pulp morphology ( J:119405 )

• disrupted compartmentalization of red pulp

abnormal spleen white pulp morphology ( J:119405 )

• disrupted compartmentalization of white pulp

intermingled spleen red and white pulp ( J:119405 )

• moribund mice exhibit complete loss of splenic compartmentalization

decreased spleen B cell follicle number ( J:119405 )

• decrease in number of follicles

abnormal lymph node morphology ( J:119405 )

• although not enlarged, lymph nodes exhibit progressive hypercellularity

• pseudo-Gaucher cells observed in lymph nodes

• increase in GR-1+ myeloid cells as compared to controls

dermatitis ( J:119405 )

• myeloid infiltrates observed in skin of moribund mice

neoplasm

increased chronic myelocytic leukemia incidence ( J:119405 )

• mice older than 10 weeks develop a malignant myeloproliferative disease at 100% penetrance that can progress to transplantable leukemia

integument

dermatitis ( J:119405 )

• myeloid infiltrates observed in skin of moribund mice

Genotype
MGI:2429456

hm3

• Allelic Composition: Alox15^tm1Fun/Alox15^tm1Fun
• Genetic Background: involves: 129/Sv * C57BL/6

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:44778 )

N • no defect detected in pituitary gland, adrenals, and pancreas

hematopoietic system

hematopoietic system phenotype ( J:44778 )

N • mutants had normal total blood cell counts, normal leukocyte differentials, normal reticulocyte numbers, normal hemoglobin values, and normal hematocrit values

immune system

abnormal immune system physiology ( J:44778 )

• altered arachadonic acid metabolism in peritoneal macrophages upon calcium ionophore stimulation: undetectable 12-hydroxyeicosatetraenoic acid (12-HETE), trace amounts of 15-hydroxyeicosatetraenoic acid (15-HETE), increased concentrations of 5-hydroxyeicosatetraenoic acid (5-HETE), however no defect detected in the spleen

reproductive system

reproductive system phenotype ( J:44778 )

N • mutants were fertile

Genotype
MGI:4367211

cx4

• Allelic Composition: Alox15^tm1Fun/Alox15^tm1Fun; Ldlr^tm1Her/Ldlr^tm1Her
• Genetic Background: involves: 129S2/SvPas * 129S7/SvEvBrd * C57BL/6

cardiovascular system

decreased susceptibility to atherosclerosis ( J:86385 )

• reduced atherosclerotic lesions when fed a high fat diet for 3 or 9 weeks