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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Ggta1tm1Jbl
targeted mutation 1, John B Lowe
MGI:1857435
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Ggta1tm1Jbl/Ggta1tm1Jbl involves: 129S2/SvPas MGI:3694706
hm2
 		Ggta1tm1Jbl/Ggta1tm1Jbl involves: 129S2/SvPas * C57BL/6J * DBA/2J MGI:3640394
cx3
 		Ggta1tm1Jbl/Ggta1+
Ightm1Jiac/Ightm1Jiac
Igktm1Jiac/Igk+ 		involves: 129S2/SvPas * 129S4/SvJae * C57BL/6 MGI:3779041
cx4
 		Ggta1tm1Jbl/Ggta1+
Ightm1Jiac/Igh+
Igktm1Jiac/Igktm1Jiac 		involves: 129S2/SvPas * 129S4/SvJae * C57BL/6 MGI:3779042
cx5
 		Ggta1tm1Jbl/Ggta1+
Ightm1Jiac/Ightm1Jiac
Igktm1Jiac/Igktm1Jiac 		involves: 129S2/SvPas * 129S4/SvJae * C57BL/6 MGI:3779044
cx6
 		Cr2tm1Crr/Cr2tm1Crr
Ggta1tm1Jbl/Ggta1tm1Jbl 		involves: 129S2/SvPas * 129S7/SvEvBrd MGI:3694708


Genotype
MGI:3694706
hm1
Allelic
Composition		 Ggta1tm1Jbl/Ggta1tm1Jbl
Genetic
Background		 involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ggta1tm1Jbl mutation (2 available); any Ggta1 mutation (81 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal humoral immune response ( J:90851 , J:113488 )
• mutants with pre-existing anti-alpha-Gal antibodies show a median graft survival time of 14 days when ectopically grafted with C57BL/6 hearts; nae mutants with no alpha-Gal antibodies show a graft survival time of >75 days; no rapid graft rejection resembling hyperacute rejection (HAR) seen in humans is observed even with sizeable anti-alpha-Gal IgM antibody titers (J:90851)
• mice can produce antibodies which bind alpha-Gal without need for immunization (J:113488)
• presence of these antibodies increases B and T cell responses to poorly immunogenic antigens expressing alpha-Gal (J:113488)
abnormal immunoglobulin level ( J:113488 )
• significant alpha-BSA-specific IgG antibody levels are observed after immunization of mutants with BSA conjugated to alpha-Gal; antibody production does not require adjuvant
decreased IgG level ( J:90851 )
• anti-alpha-Gal IgG titers are low or not detectable in mutants before or after LLCa challenge; antibodies are found in only 33% of orally-immunized mutants pre-LLCa challenge
increased IgM level ( J:90851 )
• naive mice challenged with Lewis Lung Carcinoma cells (LLCa) show production of LLCa-reactive antibodies
• in orally immunized mutants, post-challenge titers are constant or increased from pre-challenge; higher percentage of immunized mice display higher IgM titers post-challenge than nae mice
abnormal response to transplant ( J:90851 )
• mutants with pre-existing anti-alpha-Gal antibodies show a median graft survival time of 14 days when ectopically grafted with C57BL/6 hearts; naive mutants with no alpha-Gal antibodies show a graft survival time of >75 days; no rapid graft rejection resembling hyperacute rejection (HAR) seen in humans is observed even with sizeable anti-alpha-Gal IgM antibody titers

neoplasm
decreased lung tumor incidence ( J:90851 )
• mutant mice with higher anti-alpha-Gal IgM antibody titers at time of tumor (LLCa) challenge show delayed tumor onset (~20 days) compared to mice with low or no IgM antibodies at challenge (~12 days) suggesting tumor onset and formation times are IgM Ab-concentration dependent

hematopoietic system
abnormal immunoglobulin level ( J:113488 )
• significant alpha-BSA-specific IgG antibody levels are observed after immunization of mutants with BSA conjugated to alpha-Gal; antibody production does not require adjuvant
decreased IgG level ( J:90851 )
• anti-alpha-Gal IgG titers are low or not detectable in mutants before or after LLCa challenge; antibodies are found in only 33% of orally-immunized mutants pre-LLCa challenge
increased IgM level ( J:90851 )
• naive mice challenged with Lewis Lung Carcinoma cells (LLCa) show production of LLCa-reactive antibodies
• in orally immunized mutants, post-challenge titers are constant or increased from pre-challenge; higher percentage of immunized mice display higher IgM titers post-challenge than nae mice

respiratory system
decreased lung tumor incidence ( J:90851 )
• mutant mice with higher anti-alpha-Gal IgM antibody titers at time of tumor (LLCa) challenge show delayed tumor onset (~20 days) compared to mice with low or no IgM antibodies at challenge (~12 days) suggesting tumor onset and formation times are IgM Ab-concentration dependent




Genotype
MGI:3640394
hm2
Allelic
Composition		 Ggta1tm1Jbl/Ggta1tm1Jbl
Genetic
Background		 involves: 129S2/SvPas * C57BL/6J * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ggta1tm1Jbl mutation (2 available); any Ggta1 mutation (81 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
normal phenotype
no abnormal phenotype detected ( J:28755 )
• mice are viable and fertile
• no differences are observed in fertility or litter size are observed compared to wild-type




Genotype
MGI:3779041
cx3
Allelic
Composition		 Ggta1tm1Jbl/Ggta1+
Ightm1Jiac/Ightm1Jiac
Igktm1Jiac/Igk+
Genetic
Background		 involves: 129S2/SvPas * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ggta1tm1Jbl mutation (2 available); any Ggta1 mutation (81 available)
Ightm1Jiac mutation (0 available); any Igh mutation (44 available)
Igktm1Jiac mutation (0 available); any Igk mutation (26 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased mature B cell number ( J:133032 )
• the frequency of B220+ B cells in the periphery is 18.7% compared to 24.7% in mice with just one Igh knock-in allele

hematopoietic system
decreased mature B cell number ( J:133032 )
• the frequency of B220+ B cells in the periphery is 18.7% compared to 24.7% in mice with just one Igh knock-in allele




Genotype
MGI:3779042
cx4
Allelic
Composition		 Ggta1tm1Jbl/Ggta1+
Ightm1Jiac/Igh+
Igktm1Jiac/Igktm1Jiac
Genetic
Background		 involves: 129S2/SvPas * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ggta1tm1Jbl mutation (2 available); any Ggta1 mutation (81 available)
Ightm1Jiac mutation (0 available); any Igh mutation (44 available)
Igktm1Jiac mutation (0 available); any Igk mutation (26 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased mature B cell number ( J:133032 )
• the frequency of B220+ B cells in the periphery is 10.1% compared to 24.7% in mice with just one Igk knock-in allele

hematopoietic system
decreased mature B cell number ( J:133032 )
• the frequency of B220+ B cells in the periphery is 10.1% compared to 24.7% in mice with just one Igk knock-in allele




Genotype
MGI:3779044
cx5
Allelic
Composition		 Ggta1tm1Jbl/Ggta1+
Ightm1Jiac/Ightm1Jiac
Igktm1Jiac/Igktm1Jiac
Genetic
Background		 involves: 129S2/SvPas * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ggta1tm1Jbl mutation (2 available); any Ggta1 mutation (81 available)
Ightm1Jiac mutation (0 available); any Igh mutation (44 available)
Igktm1Jiac mutation (0 available); any Igk mutation (26 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal B cell selection ( J:133032 )
• anti-alphaGal IgM is detectable in the sera of mice
• the frequency of B cells expressing anti-alphaGal IgM in the spleen is higher than controls
decreased mature B cell number ( J:133032 )
• the frequency of B220+ B cells in the periphery is 6.6% compared to 24.7% in mice with just one knock-in allele for Igk and Igh
abnormal B cell physiology ( J:133032 )
• the anti-alphaGal IgM produced by B cells has a lower affinity for antigen than immunoglobulins produced in mice with with no endogenous expression of alphaGal

immune system
abnormal B cell selection ( J:133032 )
• anti-alphaGal IgM is detectable in the sera of mice
• the frequency of B cells expressing anti-alphaGal IgM in the spleen is higher than controls
decreased mature B cell number ( J:133032 )
• the frequency of B220+ B cells in the periphery is 6.6% compared to 24.7% in mice with just one knock-in allele for Igk and Igh
abnormal B cell physiology ( J:133032 )
• the anti-alphaGal IgM produced by B cells has a lower affinity for antigen than immunoglobulins produced in mice with with no endogenous expression of alphaGal




Genotype
MGI:3694708
cx6
Allelic
Composition		 Cr2tm1Crr/Cr2tm1Crr
Ggta1tm1Jbl/Ggta1tm1Jbl
Genetic
Background		 involves: 129S2/SvPas * 129S7/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cr2tm1Crr mutation (0 available); any Cr2 mutation (84 available)
Ggta1tm1Jbl mutation (2 available); any Ggta1 mutation (81 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal humoral immune response ( J:113488 )
• double mutants do not produce alpha-Gal-reactive natural antibodies; Ggta1-null and double mutants given anti alpha-Gal serumproduce similar levels of BSA-specific IgG antibodies after alpha-Gal immunization, indicating that complement receptors are not required to produce increased carrier immunogenicity
• elevated BSA immunogenicity in mutants is directly related to alpha-Gal specific antibodies




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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory