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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Gria2tm1Rod
targeted mutation 1, John Roder
MGI:1857436
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Gria2tm1Rod/Gria2tm1Rod B6.129-Gria2tm1Rod/J MGI:4834610
hm2
 		Gria2tm1Rod/Gria2tm1Rod involves: 129S1/Sv * 129X1/SvJ * CD-1 MGI:2176810
ht3
 		Gria2tm1Rod/Gria2+ B6.129-Gria2tm1Rod/J MGI:4834611
cx4
 		Gria2tm1Rod/Gria2tm1Rod
Tg(SOD1*G93A)1Gur/0 		B6.Cg-Gria2tm1Rod Tg(SOD1*G93A)1Gur MGI:4834606
cx5
 		Gria2tm1Rod/Gria2+
Tg(SOD1*G93A)1Gur/0 		B6.Cg-Gria2tm1Rod Tg(SOD1*G93A)1Gur MGI:4834607
cx6
 		Gria2tm1Rod/Gria2tm1Rod
Gria3tm1Zpj/Y 		involves: 129/Sv * C57BL/6 MGI:2674947


Genotype
MGI:4834610
hm1
Allelic
Composition		 Gria2tm1Rod/Gria2tm1Rod
Genetic
Background		 B6.129-Gria2tm1Rod/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gria2tm1Rod mutation (2 available); any Gria2 mutation (79 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
increased susceptibility to neuronal excitotoxicity ( J:104951 )
• increased vulnerability to AMPA receptor-mediated excitotoxicity
• abolished AMPA receptor-mediated cell death in cultured motor neurons by the external polyamine 1-naphtyl acetyl spermine (NAS)
abnormal AMPA-mediated synaptic currents ( J:104951 )
• high degree of inhibition by the external NAS, a strong inward rectification and a high relative Ca2+ permeability
• higher elevations of the intracellular Ca2+ concentration upon AMPA receptor stimulation
• normal number of AMPA receptors in the cell membrane

behavior/neurological
abnormal response to novel object ( J:104951 )
• reduced object exploration
impaired coordination ( J:104951 )
• unable to walk on a rotarod
decreased grip strength ( J:104951 )
• slightly lower grip strength in fore limbs
abnormal limb posture ( J:104951 )
• abnormal posturing of both forelimbs and hind limbs during manipulations
decreased locomotor activity ( J:104951 )
• reduced spontaneous activity during the night

homeostasis/metabolism
increased susceptibility to neuronal excitotoxicity ( J:104951 )
• increased vulnerability to AMPA receptor-mediated excitotoxicity
• abolished AMPA receptor-mediated cell death in cultured motor neurons by the external polyamine 1-naphtyl acetyl spermine (NAS)

cellular
increased susceptibility to neuronal excitotoxicity ( J:104951 )
• increased vulnerability to AMPA receptor-mediated excitotoxicity
• abolished AMPA receptor-mediated cell death in cultured motor neurons by the external polyamine 1-naphtyl acetyl spermine (NAS)




Genotype
MGI:2176810
hm2
Allelic
Composition		 Gria2tm1Rod/Gria2tm1Rod
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gria2tm1Rod mutation (2 available); any Gria2 mutation (79 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
postnatal lethality, incomplete penetrance ( J:64275 )
• by 2 - 3 weeks of age about 20% of homozygotes die

behavior/neurological
abnormal response to novel object ( J:64275 )
• object exploration is reduced
abnormal reflex ( J:64275 )
• impaired eye closure reflex to approaching objects
impaired coordination ( J:64275 )
• impaired coordination on rotorod test; however no signs of seizure activity were detected by observation, EEG, or post-mortem pyramidal cell dropout in the hippocampus

nervous system
enhanced long-term potentiation ( J:64275 )
• LTP in CA1 region of the hippocampus is enhanced 2-fold and non-saturating

growth/size/body
decreased body size ( J:64275 )
• body size is reduced at 2 - 3 weeks of age; however by 6 - 7 weeks, surviving homozygotes were the same size as littermates
decreased body weight ( J:64275 )
• body weight is reduced at 2 - 3 weeks of age; however by 6 - 7 weeks, surviving homozygotes were the same weight as littermates




Genotype
MGI:4834611
ht3
Allelic
Composition		 Gria2tm1Rod/Gria2+
Genetic
Background		 B6.129-Gria2tm1Rod/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gria2tm1Rod mutation (2 available); any Gria2 mutation (79 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
increased susceptibility to neuronal excitotoxicity ( J:104951 )
• increased vulnerability to AMPA receptor-mediated excitotoxicity
abnormal AMPA-mediated synaptic currents ( J:104951 )
• high degree of inhibition by the external polyamine 1-naphtyl acetyl spermine (NAS), a strong inward rectification and a high relative Ca2+ permeability
• higher elevations of the intracellular Ca2+ concentration upon AMPA receptor stimulation
• normal number of AMPA receptors in the cell membrane

homeostasis/metabolism
increased susceptibility to neuronal excitotoxicity ( J:104951 )
• increased vulnerability to AMPA receptor-mediated excitotoxicity

cellular
increased susceptibility to neuronal excitotoxicity ( J:104951 )
• increased vulnerability to AMPA receptor-mediated excitotoxicity




Genotype
MGI:4834606
cx4
Allelic
Composition		 Gria2tm1Rod/Gria2tm1Rod
Tg(SOD1*G93A)1Gur/0
Genetic
Background		 B6.Cg-Gria2tm1Rod Tg(SOD1*G93A)1Gur
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gria2tm1Rod mutation (2 available); any Gria2 mutation (79 available)
Tg(SOD1*G93A)1Gur mutation (4 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:104951 )
• shortened lifespan (116.50.6 vs. 137.12.6 days), compare with Tg(SOD1*G93A)1Gur mice

behavior/neurological
impaired coordination ( J:104951 )
• unable to walk on a rotarod
decreased grip strength ( J:104951 )
• decreased grip strength in forelimbs
hindlimb paralysis ( J:104951 )
• earlier onset (99.32.1 days vs. 117.31.8 days), compare with Tg(SOD1*G93A)1Gur mice

nervous system
decreased motor neuron number ( J:104951 )
• decreased number of the large neurons in the ventral horn of the lumbar spinal cord
abnormal AMPA-mediated synaptic currents ( J:104951 )
• higher elevations of the intracellular Ca2+ concentration upon AMPA receptor stimulation




Genotype
MGI:4834607
cx5
Allelic
Composition		 Gria2tm1Rod/Gria2+
Tg(SOD1*G93A)1Gur/0
Genetic
Background		 B6.Cg-Gria2tm1Rod Tg(SOD1*G93A)1Gur
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gria2tm1Rod mutation (2 available); any Gria2 mutation (79 available)
Tg(SOD1*G93A)1Gur mutation (4 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
decreased motor neuron number ( J:104951 )
• decreased number of the large neurons in the ventral horn of the lumbar spinal cord




Genotype
MGI:2674947
cx6
Allelic
Composition		 Gria2tm1Rod/Gria2tm1Rod
Gria3tm1Zpj/Y
Genetic
Background		 involves: 129/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gria2tm1Rod mutation (2 available); any Gria2 mutation (79 available)
Gria3tm1Zpj mutation (0 available); any Gria3 mutation (12 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
postnatal lethality, incomplete penetrance ( J:84336 )
• normal at birth
• increased mortality at 2 to 4 weeks of age
• to about 20-30%
• gradual increase of global abnormalities

behavior/neurological
tremors ( J:84336 )
• severe tremors associated with movements

nervous system
nervous system phenotype ( J:84336 )
N
• no gross anatomic abnormalities seen in the CNS
• synaptic structures in the CA1 region appear normal
abnormal synaptic plasticity ( J:84336 )
• synaptic plasticity was greater
abnormal excitatory postsynaptic potential ( J:84336 )
• field excitatory postsynaptic potential was reduced to 10-20% of wild-type and was significantly lower than in Gria2 mutant homozygotes
enhanced long-term potentiation ( J:84336 )
• in the CA1 region
abnormal long-term depression ( J:84336 )
• in the CA1 region LTD is enhanced
• limited effect of D15 peptide in blocking LTD

growth/size/body
decreased body size ( J:84336 )




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory